重组Anti-Arp3抗体[EPR10429] - C-terminal (ab181164)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10429] to Arp3 - C-terminal
- Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
-
产品名称
Anti-Arp3抗体[EPR10429] - C-terminal
参阅全部 Arp3 一抗 -
描述
兔单克隆抗体[EPR10429] to Arp3 - C-terminal -
宿主
Rabbit -
特异性
The rat recommendation is based on the WB results. We do not guarantee IHC-P for rat.
-
经测试应用
适用于: Flow Cyt (Intra), IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- WB: HeLa, Jurkat, A549, Mouse spleen, Rat spleen, and Mouse brain lysates. IHC-P: human kidney, and mouse kidney tissues. ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR10429 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab181164于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/30.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For unpurified please use at 1/40 |
|
IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
The rat recommendation is based on the WB results. We do not guarantee IHC-P for rat. |
|
WB |
1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
|
|
ICC/IF |
1/50 - 1/100.
|
说明 |
---|
Flow Cyt (Intra)
1/30. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For unpurified please use at 1/40 |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. The rat recommendation is based on the WB results. We do not guarantee IHC-P for rat. |
WB
1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). |
ICC/IF
1/50 - 1/100. |
靶标
-
相关性
The Arp2/3 complex involved in Golgi polerization in NIH3T3 cells. In a different model it has been shown that the Arp2/3 complex is necessary for neutrophil chemotaxis and phagocytosis. -
细胞定位
Cytoplasmic -
数据库链接
- Entrez Gene: 10096 Human
- Omim: 604222 Human
- SwissProt: P61158 Human
-
别名
- Actin like protein 3 antibody
- Actin related protein 3 antibody
- ACTR3 antibody
- ARP3 actin related protein 3 homolog (yeast) antibody
图片
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling Arp3 with purified ab181164 at 1:250 dilution (1.01 µg/ml). Heat mediated antigen retrieval was performed using Bond ™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
-
Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Arp3 with purified ab181164 at 1:50 dilution (5.06 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
-
Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Arp3 with purified ab181164 at 1/30 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling Arp3 with purified ab181164 at 1:250 dilution (1.01 µg/ml). Heat mediated antigen retrieval was performed using Bond ™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
-
All lanes : Anti-Arp3 antibody [EPR10429] - C-terminal (ab181164) at 1/1000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Mouse spleen lysates
Lane 3 : Rat spleen lysates
Lane 4 : Mouse brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa -
All lanes : Anti-Arp3 antibody [EPR10429] - C-terminal (ab181164) at 1/10000 dilution (Unpurified)
Lane 1 : HeLa lysate
Lane 2 : Jurkat lysate
Lane 3 : A549 lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 47 kDa -
Immunofluorescence analysis of -20°C Acetone fixed HeLa cells labeling Arp3 with unpurified ab181164 at a 1/100 dilution. Goat anti rabbit IgG (Alexa Fluor® 488) secondary used at a 1/200 diution.
-
Immunohistochemical analysis of paraffin embedded human kidney tissue labeling Arp3 with unpurified ab181164 at a 1/250 dilution. Prediluted HRP Rabbit IgG secondary was used. Counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
Certificate of Compliance
文献 (3)
ab181164 被引用在 3 文献中.
- Li Y et al. Dragon's Blood Regulates Rac1-WAVE2-Arp2/3 Signaling Pathway to Protect Rat Intestinal Epithelial Barrier Dysfunction Induced by Simulated Microgravity. Int J Mol Sci 22:N/A (2021). PubMed: 33800361
- Liu Y et al. JMY expression by Sertoli cells contributes to mediating spermatogenesis in mice. FEBS J 287:5478-5497 (2020). PubMed: 32279424
- Wu D et al. Olaquindox disrupts tight junction integrity and cytoskeleton architecture in mouse Sertoli cells. Oncotarget 8:88630-88644 (2017). WB ; Mouse . PubMed: 29179463