Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Spinal cord)
Antigen retrieval step
None
Permeabilization
Yes - TritonX 100
Specification
Spinal cord
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS with 0.1% Triton-X 100
Dilution
1/100
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Donkey anti-Rabbit IgG (H+L) Secondary Antibody, A
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Sep 29 2015