参阅全部 Aromatase 一抗
特异性ab18995 recognises aromatase.
经测试应用适用于: ICC/IF, IHC-Fr, IHC-P, WBmore details
种属反应性与反应: Mouse, Rat, Human
预测可用于: Chicken, Cow, Pig
Synthetic peptide surrounding amino acid 385 of human Aromatase (Peptide available as ab51924.)
- ICC/IF: HepG2 cells. IHC-P: Human normal placenta.
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
存储溶液Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 30% Glycerol, 0.5% BSA
Concentration information loading...
纯度Immunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab18995 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 19620711|
|IHC-P||Use a concentration of 10 - 20 µg/ml.|
|WB||Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 58 kDa). An additional 35 kDa band can also be detected in Jurkat cells (we are unsure of the identity).|
功能Catalyzes the formation of aromatic C18 estrogens from C19 androgens.
组织特异性Brain, placenta and gonads.
疾病相关Defects in CYP19A1 are a cause of aromatase excess syndrome (AEXS) [MIM:139300]; also known as familial gynecomastia. AEXS is characterized by an estrogen excess due to an increased aromatase activity.
Defects in CYP19A1 are the cause of aromatase deficiency (AROD) [MIM:107910]. AROD is a rare disease in which fetal androgens are not converted into estrogens due to placental aromatase deficiency. Thus, pregnant women exhibit a hirsutism, which spontaneously resolves after post-partum. At birth, female babies present with pseudohermaphroditism due to virilization of extern genital organs. In adult females, manifestations include delay of puberty, breast hypoplasia and primary amenorrhoea with multicystic ovaries.
序列相似性Belongs to the cytochrome P450 family.
- Information by UniProt
- ARO antibody
- ARO1 antibody
- Aromatase antibody
Ab18995 staining Human normal placenta. Staining is localised to cell membrane.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
ICC/IF image of ab18995 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18995, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-Aromatase antibody (ab18995) at 1 µg/ml + Human brain normal tissue lysate - membrane extract (ab29456) at 10 µg
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 58 kDa
Observed band size: 58 kDa
Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
This product has been referenced in:
- Bogus K et al. Chronic Antipsychotic Treatment Modulates Aromatase (CYP19A1) Expression in the Male Rat Brain. J Mol Neurosci 68:311-317 (2019). Read more (PubMed: 30968339) »
- Berthon A et al. High expression of adrenal P450 aromatase (CYP19A1) in association with ARMC5-primary bilateral macronodular adrenocortical hyperplasia. J Steroid Biochem Mol Biol 191:105316 (2019). Read more (PubMed: 31014964) »