Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Mouse Cell lysate - whole cell (NIH3T3 cells)
Specification
NIH3T3 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: TBST
Dilution
1/1500
Secondary antibody
Name
Non-Abcam antibody was used: Anti-Goat IgG (whole molecule)-Alkaline Phosphatas
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Dilution
1/15000
Detection
Detection method
ECL+
Bands
Specific: 65 kDa Non-specific: 45, 50 kDa
Exposure
15 minute(s) and 0 second(s)
Additional data
Additional Notes
IMAGE LEGEND
lane 1: 30 ug whole NIH3T3 lysate, 12% SDS-PAGE, WB developed with colorimetric substrate
lane 2: 30 ug whole NIH3T3 lysate, 12% SDS-PAGE, WB developed with ECF+
lane 3: 10 ug partially purified NIH3T3 protein mixture, 4-15% SDS-PAGE, WB developed with colorimetric substrate
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. T Lawson
Verified customer
提交于 Sep 16 2013