Anti-Argonaute 5抗体(ab4723)
Key features and details
- Rabbit polyclonal to Argonaute 5
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
概述
-
产品名称
Anti-Argonaute 5抗体 -
描述
兔多克隆抗体to Argonaute 5 -
宿主
Rabbit -
特异性
ab4723 detects a band of ~ 75 kDa in P19 embryonic carcinoma lysate. The predicted molecular weight of murine Argonaute 5 is 72 kDa according to Swissprot entry Q8CGT7. (This represents a partial sequence) Other bands of 85, 160 and 250 kDa are also detected which may be non-specific binding or alternatively may correspond to the full length Argonaute 5. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse -
免疫原
Synthetic peptide corresponding to Mouse Argonaute 5 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab96298) -
阳性对照
- This antibody gave a positive signal in F9 whole cell lysate.
-
常规说明
Swiss prot Q8CGT7 - fragmentReproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
Our Abpromise guarantee covers the use of ab4723 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 72 kDa). |
靶标
-
相关性
Argonaute proteins make up a highly conserved family whose members have been implicated in RNAi and related phenomena in several organisms. In addition to roles in RNAi-like mechanisms, Argonaute proteins influence development, and at least a subset are involved in stem cell fate determination. Argonaute proteins are ~100-kD highly basic proteins that contain two common domains, namely PAZ and PIWI domains (Cerutti et al. 2000). The PAZ domain, consisting of 130 amino acids, has been identified in Argonaute proteins and in Dicer (Bernstein et al. 2001a). Although it has no defined function, PAZ is thought to be a protein-protein interaction domain, potentially mediating both homo- and heterodimerization (Cerutti et al. 2000). There are several reports that Argonaute proteins, being highly basic, might bind RNA. Miwi, a murine homolog of Piwi, exists in a complex with its target mRNAs in vivo (Deng and Lin 2002). Furthermore, Drosophila Ago1 has been shown to bind ribohomopolymers in vitro (Kataoka et al. 2001). Of course, the involvement of Argonaute proteins in RNAi and related phenomena presupposes an interaction with RNA, either directly or indirectly. However, detailed studies of Argonaute structure and biochemistry will be required to reveal its precise function in RNA metabolism. -
细胞定位
Nuclear -
数据库链接
- Entrez Gene: 286951 Mouse
- SwissProt: Q8CGT7 Mouse
-
别名
- Ago 5 antibody
- Ago5 antibody
- Argonaute 5 protein antibody
see all
图片
-
Western blot - Anti-Argonaute 5 antibody (ab4723)This image was submitted as part or a review by Aarti Jagannath
30µg of p19 murine embryonic carcinoma cell lysate was run on an SDS-PAGE and incubated for 12 hours with ab4723 (1/1000 dilution). A specific band of ~ 75 kDa is detected.
-
Western blot - Anti-Argonaute 5 antibody (ab4723)Anti-Argonaute 5 antibody (ab4723) at 1 µg/ml + F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 72 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
数据表及文件
文献 (0)
ab4723 尚未被引用在任何文献中。
