Anti-ARF1抗体(ab58578)
Key features and details
- Goat polyclonal to ARF1
- Suitable for: ELISA, Flow Cyt, WB
- Knockout validated
- Reacts with: Mouse, Rat, Cow, Dog, Human
- Isotype: IgG
概述
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产品名称
Anti-ARF1抗体
参阅全部 ARF1 一抗 -
描述
山羊多克隆抗体to ARF1 -
宿主
Goat -
经测试应用
适用于: ELISA, Flow Cyt, WBmore details -
种属反应性
与反应: Mouse, Rat, Cow, Dog, Human
预测可用于: Xenopus laevis, Cynomolgus monkey -
免疫原
Synthetic peptide:
EGLDWLSNQLRNQK
by a Cysteine residue linker, corresponding to C terminal amino acids 168-181 of ARF1 -
阳性对照
- WB: A431, HeLa, HepG2, MDCK, NIH3T3 KNRK cell lysate. Flow Cyt: A431 cells
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常规说明
This antibody is expected to recognize all four reported isoforms.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Primary antibody说明
This antibody is expected to recognize all four reported isoforms. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Assay kits
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab58578于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ELISA |
Use at an assay dependent concentration.
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Flow Cyt |
Use a concentration of 10 µg/ml.
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WB | (4) |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 21 kDa).
1 hour primary incubation is recommended for this product. |
说明 |
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ELISA
Use at an assay dependent concentration. |
Flow Cyt
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 21 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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功能
GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. -
序列相似性
Belongs to the small GTPase superfamily. Arf family. -
细胞定位
Golgi apparatus. Cytoplasm > perinuclear region. - Information by UniProt
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数据库链接
- Entrez Gene: 338058 Cow
- Entrez Gene: 100856517 Dog
- Entrez Gene: 375 Human
- Entrez Gene: 11840 Mouse
- Entrez Gene: 64310 Rat
- Entrez Gene: 380166 Xenopus laevis
- Omim: 103180 Human
- SwissProt: P84080 Cow
see all -
别名
- ADP Ribosylation Factor 1 antibody
- ADP-ribosylation factor 1 antibody
- ARF 1 antibody
see all
图片
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All lanes : Anti-ARF1 antibody (ab58578) at 1 µg/ml
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : ARF1 knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?Lanes 1 - 2: Merged signal (red and green). Green - ab58578 observed at 18 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.
ab58578 was shown to react with ARF1 in wild-type HeLa cells in Western blot with loss of signal observed in ARF1 knockout cell line ab264939 (ARF1 knockout cell lysate ab257353). Wild-type HeLa and ARF1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab58578 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at 1 µg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Donkey anti-Goat IgG H&L (IRDye® 800CW) preabsorbed (ab216775) and Donkey anti-Mouse 680RD secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Lane 1 : Anti-ARF1 antibody (ab58578) at 1 µg/ml
Lanes 2-3 : Anti-ARF1 antibody (ab58578) at 0.03 µg/ml
Lane 1 : A431 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : MCF7 cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 21 kDaLysates in RIPA buffer. Detected by chemiluminescence.
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All lanes : Anti-ARF1 antibody (ab58578) at 1 µg/ml
Lane 1 : NIH3T3 cell lysate
Lane 2 : KNRK cell lysate
Lane 3 : MDCK cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 21 kDaLysates in RIPA buffer. Detected by chemiluminescence.
数据表及文件
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Datasheet download
文献 (11)
ab58578 被引用在 11 文献中.
- Chui AJ et al. Activation of the CARD8 Inflammasome Requires a Disordered Region. Cell Rep 33:108264 (2020). PubMed: 33053349
- Mangleburg CG et al. Integrated analysis of the aging brain transcriptome and proteome in tauopathy. Mol Neurodegener 15:56 (2020). PubMed: 32993812
- Navare AT et al. Viral protein engagement of GBF1 induces host cell vulnerability through synthetic lethality. bioRxiv N/A:N/A (2020). PubMed: 33173868
- Steiner B et al. ER remodeling by the large GTPase atlastin promotes vacuolar growth of Legionella pneumophila. EMBO Rep 18:1817-1836 (2017). PubMed: 28835546
- Escoll P et al. Legionella pneumophila Modulates Mitochondrial Dynamics to Trigger Metabolic Repurposing of Infected Macrophages. Cell Host Microbe 22:302-316.e7 (2017). PubMed: 28867389