Key features and details
- Rabbit polyclonal to ARAP1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
经测试应用适用于: WB, IPmore details
预测可用于: Rabbit, Cow, Dog, Pig, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant
Synthetic peptide within Human ARAP1 aa 1150 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_056057.1
Database link: Q96P48-1
- HeLa whole cell lysate.
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
纯度Immunogen affinity purified
功能Phosphatidylinositol-3,4,5-trisphosphate-dependent GTPase-activating protein that modulates actin cytoskeleton remodeling by regulating ARF and RHO family members. Is activated by phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) binding. Can be activated by phosphatidylinositol-3,4-bisphosphate (PtdIns(3,4,5)P2) binding, albeit with lower efficiency. Has a preference for ARF1 and ARF5.
组织特异性Detected in heart, skeletal muscle, spleen, kidney, liver, placenta, lung, peripheral blood leukocytes, adrenal gland, bone marrow, brain, lymph node, mammary gland, prostate, spinal cord, stomach, thyroid and trachea.
序列相似性Contains 1 Arf-GAP domain.
Contains 4 PH domains.
Contains 1 Ras-associating domain.
Contains 1 Rho-GAP domain.
Contains 1 SAM (sterile alpha motif) domain.
细胞定位Cytoplasm. Golgi apparatus > Golgi stack membrane. Cell membrane. Associated with Golgi stacks in resting cells. Throughout the cytoplasm and in surface protrusion in cells that are in the process of attaching to a surface and spreading.
- Information by UniProt
- 2410002L19Rik antibody
- ANK repeat and PH domain-containing protein 1 antibody
- ARAP 1 antibody
All lanes : Anti-ARAP1 antibody (ab99382) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 136 kDa
Exposure time: 30 seconds
Detection of ARAP1 by Western Blot of Immunprecipitate.
ab99382 at 1µg/ml staining ARAP1 in HeLa whole cell lysate immunoprecipitated using ab99382 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
ab99382 被引用在 1 文献中.
- Hu M et al. Chromatin 3D interaction analysis of the STARD10 locus unveils FCHSD2 as a regulator of insulin secretion. Cell Rep 34:108703 (2021). PubMed: 33535042