Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (THP1 monocyte-derived macrophages)
Specification
THP1 monocyte-derived macrophages
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.25% Triton X-100 in PBS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Other product details
Dilution
1/250
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 10% BSA/0.05% Triton/0.3M glycine in PBS
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti-Rabbit AlexaFluor 488
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/300
Additional data
Additional Notes
Paraformaldehyde used: 4%
Blocking buffer: 10% BSA, 0.05% Triton, 0.3M glycine in PBS
Nuclei stained using mounting media containing dapi
Blocking buffer: 10% BSA, 0.05% Triton, 0.3M glycine in PBS
Nuclei stained using mounting media containing dapi
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Elizabeth Kilby
Verified customer
提交于 Nov 02 2012