Anti-Apolipoprotein CIII抗体(ab7619)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein CIII antibody (ab7619)

    Key features and details

    • Goat polyclonal to Apolipoprotein CIII
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

    选择批间可重复性更高的重组抗体

    Product image
    Anti-Apolipoprotein CIII antibody [EPR1371(2)] (ab108205)
    • 研究可靠 —— 各批次间结果一致且可重复
    • 长期批量供应 —— 采用重组技术，可实现快速生产
    • 首次实验即可成功 —— 经过大量验证确认了特异性
    • 符合伦理标准 —— 产品不含动物成分

    概述

    • 产品名称

      Anti-Apolipoprotein CIII抗体
      参阅全部 Apolipoprotein CIII 一抗

    • 描述

      山羊多克隆抗体to Apolipoprotein CIII

    • 宿主

      Goat

    • 特异性

      Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA. This antibody reacts with human apoLipoprotein C-III and has negligible cross-reactivity with Type A-I, A-II, B, C-I, C-II, E and J apoLipoproteins.

    • 经测试应用

      适用于: IHC-Pmore details

    • 种属反应性

      与反应: Human

    • 免疫原

      Full length native apoLipoprotein Type C-III (purified).

    • 常规说明

      This antibody has been used to determine that atherosclerotic lesions in the human aorta contain considerable amounts of lipoproteins. These lipoproteins were observed to be complexed with components of the extracellular matrix (especially LDL and proteoglycans). The role of these matrix-lipoprotein complexes is not entirely clear, however, animal models of atherosclerosis have shown that increased cellular proliferation and increased production of extracellular matrix components occur following injury to the intimal layer of the aorta.

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    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab7619于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P
    1/50 - 1/200.
    说明
    IHC-P
    1/50 - 1/200.

    靶标

    • 功能

      Inhibits lipoprotein lipase and hepatic lipase and decreases the uptake of lymph chylomicrons by hepatic cells. This suggests that it delays the catabolism of triglyceride-rich particles.

    • 组织特异性

      Constitutes 50% of the protein fraction of VLDL and 2% of that of HDL. Synthesized predominantly in liver and to a lesser degree in intestine.

    • 疾病相关

      Defects in APOC3 may be a cause of hyperalphalipoproteinemia (HYPALIP) [MIM:143470]. Affected individuals show high levels of alpha-lipoprotein (high density lipoprotein/HDL).

    • 序列相似性

      Belongs to the apolipoprotein C3 family.

    • 翻译后修饰

      O-linked glycan consists of Gal-GalNAc disaccharide, further modified with up to 3 sialic acid residues. O-glycosylated on Thr-94 with a core 1 or possibly core 8 glycan.

    • 细胞定位

      Secreted.
    • Information by UniProt

    • 数据库链接

      • 别名

        • APOC3 antibody
        • APO C3 antibody
        • Apo CIII antibody
        • Apo-CIII antibody
        • APOC 3 antibody
        • ApoC III antibody
        • ApoC-III antibody
        • APOC3 antibody
        • APOC3_HUMAN antibody
        • ApoCIII antibody
        • Apolipoprotein C III antibody
        • Apolipoprotein C-III antibody
        • Apolipoprotein C3 antibody
        • ApolipoproteinCIII antibody
        • MGC150353 antibody
        see all

      图片

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein CIII antibody (ab7619)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein CIII antibody (ab7619)
        IHC image of ab7619 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab7619, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

        For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      文献 (4)

      发表研究结果有使用 ab7619？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab7619 被引用在 4 文献中.

      • Hesse D  et al. Hepatic trans-Golgi action coordinated by the GTPase ARFRP1 is crucial for lipoprotein lipidation and assembly. J Lipid Res 55:41-52 (2014). IHC-P ; Mouse . PubMed: 24186947
      • Qu S  et al. PPAR{alpha} mediates the hypolipidemic action of fibrates by antagonizing FoxO1. Am J Physiol Endocrinol Metab 292:E421-34 (2007). WB ; Syrian hamster . PubMed: 16985262
      • Qu S  et al. Effects of apoA-V on HDL and VLDL metabolism in APOC3 transgenic mice. J Lipid Res 48:1476-87 (2007). WB ; Human . PubMed: 17438339
      • Altomonte J  et al. Foxo1 mediates insulin action on apoC-III and triglyceride metabolism. J Clin Invest 114:1493-503 (2004). WB ; Mouse . PubMed: 15546000

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      1-2 of 2 Abreviews or Q&A

      Question

      Tried on human cells and gets a single band (no background or other bands) at 29-37kD. Expected (from SwissProt - although this is not on the datasheet) is 10kD.

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      Answer

      Thank you again for your enquiry and I apologize for the delay in responding to you. There is no Western blot image available from us for this antibody. This antibody has been used and the Western images published in the following reference, however, Altomonte J et al. Foxo1 mediates insulin action on apoC-III and triglyceride metabolism. J Clin Invest 114:1493-503 (2004). PubMed: 15546000. In this reference the antibody is used on 20 ug of plasma proteins at 1/2000 and the secondary is used at 1/1000. I noticed that you have tried the secondary at 1/40,000. I am wondering if you could try the primary at 1/1000 without milk and the secondary at 1/1000 without milk. Perhaps by concentrating the secondary and removing the milk you will see the correct band. You may also benefit from contacting the corresponding author of the paper Hengjian Henry Dong, Email: dongh@pitt.edu. Please let me know if this helps and do not hesitate to contact us for further advice,

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      Question

      Hi - I hope you can help with these questions: 1. On westerns, do you see a single band or multiple bands? 2. Do you have a picture of a western? 3. Suggested western working dilutions? 4. Suggested western sample? Do you use the cytosolic fraction or some other fraction?

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      Answer

      We apply purified apolipoprotein C-III to the gel and get only a single band at 8.8 kDa. A picture is not available. tThe suggested dilution is 1/5000 to 1/10,000. Human serum would be a good western sample. I hope this information helps you. If you have any further questions please do not hesitate in contacting me.

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