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Cardiovascular Lipids / Lipoproteins Lipid Metabolism Cholesterol Metabolism

Anti-Apolipoprotein B抗体(ab7616)

  • Datasheet
  • SDS
Reviews (2)Q&A (10)References (13)

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Western blot - Anti-Apolipoprotein B antibody (ab7616)

    Key features and details

    • Goat polyclonal to Apolipoprotein B
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    选择批间可重复性更高的重组抗体

    Product image
    Anti-Apolipoprotein B antibody [EPR2914] (ab139401)
    • 研究可靠 —— 各批次间结果一致且可重复
    • 长期批量供应 —— 采用重组技术,可实现快速生产
    • 首次实验即可成功 —— 经过大量验证确认了特异性
    • 符合伦理标准 —— 产品不含动物成分

    概述

    • 产品名称

      Anti-Apolipoprotein B抗体
      参阅全部 Apolipoprotein B 一抗
    • 描述

      山羊多克隆抗体to Apolipoprotein B
    • 宿主

      Goat
    • 特异性

      Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA. This antibody reacts with human apoLipoprotein B and has negligible cross-reactivity with Type A-I, A-II, C-I, C-II, C-III, E and J apoLipoproteins.
    • 经测试应用

      适用于: WBmore details
    • 种属反应性

      与反应: Human
    • 免疫原

      Full length native apoLipoprotein Type B (purified).

    • 常规说明

      This antibody has been used to determine that atherosclerotic lesions in the human aorta contain considerable amounts of lipoproteins. These lipoproteins were observed to be complexed with components of the extracellular matrix (especially LDL and proteoglycans). The role of these matrix-lipoprotein complexes is not entirely clear, however, animal models of atherosclerosis have shown that increased cellular proliferation and increased production of extracellular matrix components occur following injury to the intimal layer of the aorta.

      At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). Abcam's apo-lipoproteins are derived from human plasma by density gradient ultracentrifugation and HPLC.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • 存储溶液

      Preservative: 0.01% Sodium azide
      Constituents: 4.7625% Sodium borate, 0.146% EDTA, 0.435% Sodium chloride
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 纯化说明

      This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities.
    • Primary antibody说明

      At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). Abcam's apo-lipoproteins are derived from human plasma by density gradient ultracentrifugation and HPLC.
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Cardiovascular
      • Lipids / Lipoproteins
      • Lipid Metabolism
      • Cholesterol Metabolism
      • Cardiovascular
      • Lipids / Lipoproteins
      • Lipoproteins/Apolipoproteins
      • Signal Transduction
      • Metabolism
      • Lipid metabolism
      • Cardiovascular
      • Atherosclerosis
      • Thrombosis
      • Fibrinolysis
      • Cardiovascular
      • Lipids / Lipoproteins
      • Lipoproteins/Apolipoproteins
      • Apolipoproteins
      • Cancer
      • Cancer Metabolism
      • Metabolic signaling pathway
      • Metabolism of lipids and lipoproteins
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Lipid metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Cholesterol Metabolism
      • Metabolism
      • Types of disease
      • Cancer
      • Metabolism
      • Types of disease
      • Heart disease

    相关产品

    • Compatible Secondaries

      • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
      • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
    • Isotype control

      • Goat IgG, polyclonal - Isotype Control (ab37373)
    • Recombinant Protein

      • Recombinant Human Apolipoprotein B (ab80037)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab7616于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    1/200 - 1/1000.
    说明
    WB
    1/200 - 1/1000.

    靶标

    • 功能

      Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
    • 疾病相关

      Hypobetalipoproteinemia, familial, 1
      Familial ligand-defective apolipoprotein B-100
      Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.
    • 序列相似性

      Contains 1 vitellogenin domain.
    • 翻译后修饰

      Palmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle.
    • 细胞定位

      Cytoplasm. Secreted.
    • Target information above from: UniProt accession P04114 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 338 Human
      • Omim: 107730 Human
      • SwissProt: P04114 Human
      • Unigene: 120759 Human
      • 别名

        • Apo B 100 antibody
        • Apo B antibody
        • Apo B-100 antibody
        • Apo B-48 antibody
        • Apo B100 antibody
        • Apo B48 antibody
        • ApoB 100 antibody
        • ApoB 48 antibody
        • APOB antibody
        • APOB_HUMAN antibody
        • Apolipoprotein B (including Ag(x) antigen) antibody
        • Apolipoprotein B 100 antibody
        • Apolipoprotein B 48 antibody
        • Apolipoprotein B antibody
        • Apolipoprotein B-48 antibody
        • Apolipoprotein B100 antibody
        • Apolipoprotein B48 antibody
        • FLDB antibody
        • LDLCQ4 antibody
        see all

      图片

      • Western blot - Anti-Apolipoprotein B antibody (ab7616)
        Western blot - Anti-Apolipoprotein B antibody (ab7616)

      实验方案

      • Western blot protocols

      Click here to view the general protocols

      数据表及文件

      • SDS download

      • Datasheet download

        Download

      文献 (13)

      发表研究结果有使用 ab7616?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab7616 被引用在 13 文献中.

      • Tougan T  et al. The malaria parasite Plasmodium falciparum in red blood cells selectively takes up serum proteins that affect host pathogenicity. Malar J 19:155 (2020). PubMed: 32295584
      • Jia H  et al. Perilipin 5 promotes hepatic steatosis in dairy cows through increasing lipid synthesis and decreasing very low density lipoprotein assembly. J Dairy Sci 102:833-845 (2019). PubMed: 30415861
      • Notarangelo M  et al. Ultrasensitive detection of cancer biomarkers by nickel-based isolation of polydisperse extracellular vesicles from blood. EBioMedicine 43:114-126 (2019). PubMed: 31047861
      • Callea F  et al. Fibrinogen Gamma Chain Mutations Provoke Fibrinogen and Apolipoprotein B Plasma Deficiency and Liver Storage. Int J Mol Sci 18:N/A (2017). PubMed: 29244742
      • Shimizu M  et al. Quercetin represses apolipoprotein B expression by inhibiting the transcriptional activity of C/EBPß. PLoS One 10:e0121784 (2015). WB ; Human . PubMed: 25875015
      View all Publications for this product

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      1-10 of 12 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Apolipoprotein B antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (Liver)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate
      Permeabilization
      No
      Specification
      Liver
      Blocking step
      Casein as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: RT°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      提交于 Nov 12 2015

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Apolipoprotein B antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Liver)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate
      Permeabilization
      No
      Specification
      Liver
      Blocking step
      Casein as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: RT°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      提交于 Nov 10 2015

      Question

      Kindly provide additional information about the conditions of WB that is shown in the image in the datasheet including the gel used.

      Read More

      Abcam community

      Verified customer

      Asked on Nov 08 2012

      Answer

      Thank you for contacting us.

      The gel that was used was Tris-Acetate. We use Invitrogen's NuPAGE 3-8% TA gels: http://products.invitrogen.com/ivgn/product/EA03755BOX
      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Abcam Scientific Support

      回复于 Nov 08 2012

      Question

      Kindly provide additional information about the conditions of WB that is shown in the image in the datasheet.

      Read More

      Abcam community

      Verified customer

      Asked on Nov 06 2012

      Answer

      Thank you for contacting us.
      I have contacted the lab asking for the protocol used to produce the WB shown on the datasheet. Here is the answer I got:
      Ab77859 was used at 0.01 ug as shown in the WB. When we used the protein at higher concentration the lane was overloaded.
      The membrane was blocked blocked for 1 hour at room temperature with 5% BSA in 0.1% TBST, with gentle rocking. Primary antibody was then diluted (1 µg/ml) with 5% BSA in 0.1% TBST and incubated overnight at 4°C with gentle rocking. The membrane was washed and incubated with Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 diluted with 5% BSA in 0.1% TBST with gentle rocking for 1 hour.
      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Abcam Scientific Support

      回复于 Nov 06 2012

      Question

      Do we have antiboduies suitable for immunoturbidimetry?
      ELISA or IP abs should work.

      Read More

      Abcam community

      Verified customer

      Asked on Aug 28 2012

      Answer

      Vielen Dank für Ihren Anruf.
      Tatsächlich hat eine meiner Kolleginnen mit der Turbidimetrie gearbeitet, und nach einiger Diskussion sind wir der Meinung, dass für Ihren Kunden Antikörper, die in ELISA und/oder IP funktionieren, geeignet sein sollte.
      Ich habe Ihnen daher folgende Produkte heraus gesucht:
      ab10241 Anti-Human Serum Albumin antibody [15C7]
      ab104453 Anti-Lipoprotein a antibody
      ab7616 Anti-Apolipoprotein B antibody
      Allerdings habe ich nichts für das CRP gefunden.
      Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.
      Benutzen Sie unsere Produkte? Schicken Sie uns einen Abreview. Verdienen Sie sich eine Belohnung!
      https://www.abcam.com/abreviews

      Read More

      Abcam Scientific Support

      回复于 Aug 28 2012

      Question

      " rel="stylesheet" />
      Dear tech,

      A customer would like to askquestion about the specificity ofab7616. Whether ab7616 could react with Apo B48 and B100? Would you please help to answer? Thank you for your help!

      Best regards,
      Zhang Yingdan

      2012-05-11





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      Read More

      Abcam community

      Verified customer

      Asked on May 14 2012

      Answer

      Thank you for contacting us.


      We have not mapped the epitope for ab7616 and have not tested the antibody against Apo B48 and B100. We have no information about the cross reactivity to Apo B48 and B100.

      I am sorry for not being able to provide you useful information this time. Please do not hesitate to contact us if you need any more advice or information in other aspects.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Abcam Scientific Support

      回复于 May 14 2012

      Question

      Answer for your questions: In immunodiffusion test we use 0.8%agar on GelBond film.Ag and Ab are applied to holes punched in agar gels.Plasma samples from mice 5ul/hole and Ab(7616)dil.1/2(5ul).After inc(ON)the precipitin bands are visualized by staining with Comassie BrilliantBlue. Question 2:no signal Question 5 : no I don´t have the batch number of the Ab which were" successful"

      Read More

      Abcam community

      Verified customer

      Asked on Apr 18 2006

      Answer

      Thank you for getting back to us with further details. I am handling Charlottes enquiries in her absence. Once again I am sorry to hear that you have been having difficulties with this antibody. The problems that you have been having with this antibody very much sound like a batch problem. I would be very grateful if you could provide me with some further details. If you could obtain some ordering information I would be able to trace the batches (good and bar) that you have used and I would certainly be prepared to provide you with a replacement vial; provided that the antibody was purchased within the past 90 days. I look forward to hearing from you.

      Read More

      Abcam Scientific Support

      回复于 Apr 19 2006

      Question

      I am interested in using one of your antibodies (ab7616) to bind LDL in media and prevent it from binding to the LDLR. Can you please tell me where the ab7616 binds to the apob100 lipoprotein and if it could be used in such a way?

      Read More

      Abcam community

      Verified customer

      Asked on Mar 22 2006

      Answer

      Thank you for your enquiry. This is a polyclonal antibody and will therefore consist of a mixed population of IgGs each of which will recognise a different epitope. I am not sure that the antibody can be used to prevent the Apo B100 antibody from binding to the LDL receptor, since the epitopes recognized by the antibody may not be on a portion of the protein involved in receptor binding. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      回复于 Mar 22 2006

      Question

      When this antibody was tested for application in Western blotting, what type of lysate was used for characterization?

      Read More

      Abcam community

      Verified customer

      Asked on Apr 11 2005

      Answer

      Thank you for your enquiry. When ab7616 was tested for application in Western blotting, it was tested against purified Apo B protein, which would be recommended as a positive control. If you have any additional questions, please contact us again.

      Read More

      Abcam Scientific Support

      回复于 Apr 12 2005

      Question

      I was wondering if the antibody to apolipoprotein B-100 that you sell is equal in fuction to the antibody to apolipoprotein B-100 produced by Polymedco (JI-H). Their antibody binds to the 51st residue of the protein. Do you have something like this?

      Read More

      Abcam community

      Verified customer

      Asked on May 06 2004

      Answer

      Thank you for your email. Ab7616 was made against the whole apo B and should be able to recognize all the residues. I'm not familiar with the antibody from Polymedco, so unfortunately can't state how this antibody works in comparison. If you have any more questions, please contact us again.

      Read More

      Abcam Scientific Support

      回复于 May 06 2004

      1-10 of 12 Abreviews or Q&A

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