Anti-Apc1 (phospho S377)抗体(ab12206)
Key features and details
- Rabbit polyclonal to Apc1 (phospho S377)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Apc1 (phospho S377)抗体
参阅全部 Apc1 一抗 -
描述
兔多克隆抗体to Apc1 (phospho S377) -
宿主
Rabbit -
特异性
ab12206 is specific for human APC1 phosphorylated at S377. Minimal reactivity is expected with the non-phosphorylated form of the protein. -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow, Dog -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab12206于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/200 - 1/1000. Detects a band of approximately 215 kDa (predicted molecular weight: 215 kDa).
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IHC-P |
Use at an assay dependent concentration.
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说明 |
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WB
1/200 - 1/1000. Detects a band of approximately 215 kDa (predicted molecular weight: 215 kDa). |
IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Belongs to the APC1 family.
Contains 4 PC repeats. -
翻译后修饰
Phosphorylated. Phosphorylation on Ser-355 occurs specifically during mitosis. - Information by UniProt
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数据库链接
- Entrez Gene: 535565 Cow
- Entrez Gene: 475750 Dog
- Entrez Gene: 64682 Human
- Entrez Gene: 17222 Mouse
- Omim: 608473 Human
- SwissProt: E1BAB6 Cow
- SwissProt: E2RLM2 Dog
- SwissProt: Q9H1A4 Human
see all -
别名
- ANAPC 1 antibody
- Anapc1 antibody
- Anaphase promoting complex subunit 1 antibody
see all
图片
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All lanes : Anti-Apc1 (phospho S377) antibody (ab12206) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate from asynchoronous cells
Lane 2 : HeLa whole cell lysate from cells treated with nocodazole
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat-anti-Rabbit IgG [H&L] MX at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 215 kDa
Observed band size: 215 kDaThe blot was probed with ab12206 overnight at 4°C
While some phosphorylated APC1 is present in untreated cells, the amount of phosphorylated protein is increased arrested in mitosis (by treatment with with nocodazole).
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ab12206 at 5.0 µg/ml staining pancreatic cancinoma cells at 60X. This image shows moderate positive cytoplasmic and occasional nuclear staining. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab12206 尚未被引用在任何文献中。