Abcam APC Conjugation Kit (ab201807) uses a simple and quick process to conjugate an antibody to APC. It can also be used to conjugate other proteins or peptides.
To conjugate an antibody to APC using this kit:
- add modifier to antibody and incubate for 3 hours
- add quencher and incubate for 30 mins
The conjugated antibody can be used immediately in WB, ELISA, IHC etc. No further purification is required and 100% of the antibody is recovered for use.
Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our rapid antibody purification and concentration kits. Use the FAQ to learn more about the technology, or about conjugating other proteins and peptides to APC.
Amount and volume of antibody for conjugation to APC
Kit size Recommended
maximum amount of antibody
30 µg 3 x 10 µg 3 x 10 µL 100 µg 1x 100 µg 1 x 100 µL 300 µg 3 x 100 µg 3 x 100 µL 1 mg 1 mg 1 mL
Buffer Requirements for Conjugation
Buffer should be pH 6.5-8.5.
Compatible buffer constituents
If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.
50mM / 0.6% Tris1 0.1% BSA2 50% glycerol 0.1% sodium azide PBS Potassium phosphate Sodium chloride HEPES Sucrose Sodium citrate EDTA Trehalose
1 Tris buffered saline is almost always ≤ 50 mM / 0.6%
2 BSA can also interfere with the use of the conjugated antibody in tissue staining.
Incompatible buffer constituents
Thiomerosal Proclin Glycine Arginine Glutathione DTT
Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.
经测试应用适用于: Conjugationmore details
存放说明Store at -20°C. Please refer to protocols.
组件 100 µg 300 µg 30 µg 1 mg APC Conjugation Mix 1 vial 3 vials 3 vials 1 vial APC Modifier Reagent 1 vial 1 vial 1 vial 1 vial APC Quencher Reagent 1 vial 1 vial 1 vial 1 vial
Our Abpromise guarantee covers the use of ab201807 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Conjugation||Use at an assay dependent concentration.|
This product has been referenced in:
- Tan HX et al. Inducible Bronchus-Associated Lymphoid Tissues (iBALT) Serve as Sites of B Cell Selection and Maturation Following Influenza Infection in Mice. Front Immunol 10:611 (2019). Read more (PubMed: 30984186) »
- Haque NS et al. CC chemokine CCL1 receptor CCR8 mediates conversion of mesenchymal stem cells to embryoid bodies expressing FOXP3+CCR8+ regulatory T cells. PLoS One 14:e0218944 (2019). Read more (PubMed: 31314754) »