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Cell Biology Cell Cycle Cell Division Spindle

Anti-APC抗体[ALi 12-28] (ab58)

  • Datasheet
  • SDS
Reviews (2)Q&A (10)References (19)

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Flow Cytometry - Anti-APC antibody [ALi 12-28] (ab58)

    Key features and details

    • Mouse monoclonal [ALi 12-28] to APC
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Isotype: IgG1

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    敲除
    Human APC knockout K-562 cell line (ab277914)

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    概述

    • 产品名称

      Anti-APC抗体[ALi 12-28]
      参阅全部 APC 一抗
    • 描述

      小鼠单克隆抗体[ALi 12-28] to APC
    • 宿主

      Mouse
    • Tested Applications & Species

      Application Species
      Flow Cyt
      Human
      See all applications and species data
    • 免疫原

      Amino acids 1-433 N-terminal fragment of human APC (Adenomutons Polyposis Coli gene Chr 5q) fused to maltose binding protein.

    • 表位

      ALI-12-28 was epitope mapped by differential in vitro expression of the N-terminal region of the APC gene by using the protein truncation test and was found to bind to APC in the region between nucleotides 135 and 422 (exons 2-3) (Efstathiou et al.).

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • 存储溶液

      Constituent: 100% PBS
    • Concentration information loading...
    • 纯度

      Protein A purified
    • 克隆

      单克隆
    • 克隆编号

      ALi 12-28
    • 骨髓瘤

      unknown
    • 同种型

      IgG1
    • 轻链类型

      unknown
    • 研究领域

      • Cell Biology
      • Cell Cycle
      • Cell Division
      • Spindle
      • Neuroscience
      • Cell Type Marker
      • Glia marker
      • Oligodendrocyte marker
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Cancer susceptibility
      • Tumor Suppressors
      • Stem Cells
      • Signaling Pathways
      • Wnt
      • Cytoplasmic
      • Cancer
      • Cell cycle
      • Cell division
      • Cancer
      • Oncoproteins/suppressors
      • Tumor suppressors
      • PTC & Wnt pathway
      • Cardiovascular
      • Heart
      • Cardiogenesis
      • Transcription factors/regulators

    相关产品

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
      • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
    • Conjugation kits

      • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
      • APC Conjugation Kit - Lightning-Link® (ab201807)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
      • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
    • KO cell lines

      • Human APC knockout K-562 cell line (ab277914)

    应用

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab58 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    应用 Species
    Flow Cyt
    Human
    应用 Ab评论 说明
    Flow Cyt
    Use 1µg for 106 cells.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

    说明
    Flow Cyt
    Use 1µg for 106 cells.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

    靶标

    • 功能

      Tumor suppressor. Promotes rapid degradation of CTNNB1 and participates in Wnt signaling as a negative regulator. APC activity is correlated with its phosphorylation state. Activates the GEF activity of SPATA13 and ARHGEF4. Plays a role in hepatocyte growth factor (HGF)-induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization.
    • 组织特异性

      Expressed in a variety of tissues.
    • 疾病相关

      Defects in APC are a cause of familial adenomatous polyposis (FAP) [MIM:175100]; which includes also Gardner syndrome (GS). FAP and GS contribute to tumor development in patients with uninherited forms of colorectal cancer. FAP is characterized by adenomatous polyps of the colon and rectum, but also of upper gastrointestinal tract (ampullary, duodenal and gastric adenomas). This is a viciously premalignant disease with one or more polyps progressing through dysplasia to malignancy in untreated gene carriers with a median age at diagnosis of 40 years.
      Defects in APC are a cause of hereditary desmoid disease (HDD) [MIM:135290]; also known as familial infiltrative fibromatosis (FIF). HDD is an autosomal dominant trait with 100% penetrance and possible variable expression among affected relatives. HDD patients show multifocal fibromatosis of the paraspinal muscles, breast, occiput, arms, lower ribs, abdominal wall, and mesentery. Desmoid tumors appears also as a complication of familial adenomatous polyposis.
      Defects in APC are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children. Although the majority of medulloblastomas occur sporadically, some manifest within familial cancer syndromes such as Turcot syndrome and basal cell nevus syndrome (Gorlin syndrome).
      Defects in APC are a cause of mismatch repair cancer syndrome (MMRCS) [MIM:276300]; also known as Turcot syndrome or brain tumor-polyposis syndrome 1 (BTPS1). MMRCS is an autosomal dominant disorder characterized by malignant tumors of the brain associated with multiple colorectal adenomas. Skin features include sebaceous cysts, hyperpigmented and cafe au lait spots.
      Defects in APC are a cause of gastric cancer (GASC) [MIM:613659]; also called gastric cancer intestinal or stomach cancer. Gastric cancer is a malignant disease which starts in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. The term gastric cancer or gastric carcinoma refers to adenocarcinoma of the stomach that accounts for most of all gastric malignant tumors. Two main histologic types are recognized, diffuse type and intestinal type carcinomas. Diffuse tumors are poorly differentiated infiltrating lesions, resulting in thickening of the stomach. In contrast, intestinal tumors are usually exophytic, often ulcerating, and associated with intestinal metaplasia of the stomach, most often observed in sporadic disease.
      Defects in APC are a cause of hepatocellular carcinoma (HCC) [MIM:114550]. This defect includes also the disease entity termed hepatoblastoma.
    • 序列相似性

      Belongs to the adenomatous polyposis coli (APC) family.
      Contains 7 ARM repeats.
    • 结构域

      The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends.
    • 翻译后修饰

      Phosphorylated by GSK3B.
      Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is facilitated by Axin. Deubiquitinated by ZRANB1/TRABID.
    • 细胞定位

      Cell junction > adherens junction. Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cell projection > ruffle membrane. Cytoplasm. Cell membrane. Associated with the microtubule network at the growing distal tip of microtubules. Accumulates in the lamellipodium and ruffle membrane in response to hepatocyte growth factor (HGF) treatment. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane.
    • Target information above from: UniProt accession P25054 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 324 Human
      • Omim: 611731 Human
      • SwissProt: P25054 Human
      • Unigene: 158932 Human
      • 别名

        • Adenomatous Polyposis Coli antibody
        • Adenomatous polyposis coli protein antibody
        • Apc antibody
        • APC_HUMAN antibody
        • CC1 antibody
        • Deleted in polyposis 2.5 antibody
        • DP2 antibody
        • DP2.5 antibody
        • DP3 antibody
        • FAP antibody
        • FPC antibody
        • GS antibody
        • Protein APC antibody
        see all

      图片

      • Flow Cytometry - Anti-APC antibody [ALi 12-28] (ab58)
        Flow Cytometry - Anti-APC antibody [ALi 12-28] (ab58)

        Overlay histogram showing HCT116 cells stained with ab58 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

      实验方案

      • Flow cytometry protocols
      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      数据表及文件

      • SDS download

      • Datasheet download

        Download

      文献 (19)

      发表研究结果有使用 ab58?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab58 被引用在 19 文献中.

      • Groß A  et al. Representing dynamic biological networks with multi-scale probabilistic models. Commun Biol 2:21 (2019). PubMed: 30675519
      • Bernkopf DB  et al. An aggregon in conductin/axin2 regulates Wnt/ß-catenin signaling and holds potential for cancer therapy. Nat Commun 10:4251 (2019). PubMed: 31534175
      • Redelman-Sidi G  et al. The Canonical Wnt Pathway Drives Macropinocytosis in Cancer. Cancer Res 78:4658-4670 (2018). PubMed: 29871936
      • Li FF  et al. Different effection of p.1125Val>Ala and rs11954856 in APC on Wnt signaling pathway. Oncotarget 8:70854-70864 (2017). PubMed: 29050326
      • Croy HE  et al. The Poly(ADP-ribose) Polymerase Enzyme Tankyrase Antagonizes Activity of the ß-Catenin Destruction Complex through ADP-ribosylation of Axin and APC2. J Biol Chem 291:12747-60 (2016). PubMed: 27068743
      View all Publications for this product

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      1-10 of 12 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-APC antibody [ALi 12-28]

      Poor
      Abreviews
      Abreviews
      This product is known to not work in this application or species.
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (mammary tumor)
      Specification
      mammary tumor
      Fixative
      Formaldehyde
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Sodium Citrate
      Permeabilization
      No
      Blocking step
      GOAT BLOCK = 10mM Tris pH 7.4, 100mM MgCl2, 0.5% Tween, 1% BSA, 5% normal goat serum (IN WATER) as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 4°C
      Read More

      Abcam user community

      Verified customer

      提交于 Mar 06 2013

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-APC antibody [ALi 12-28]

      Average
      Abreviews
      Abreviews
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Colon Tumor and Ameloblastoma)
      Specification
      Colon Tumor and Ameloblastoma
      Fixative
      10% buffered formalin
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: 10mM Citrate Buffer
      Permeabilization
      No
      Blocking step
      (agent) for 30 minute(s) · Concentration: 10% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      提交于 Dec 03 2010

      Question

      I am doing an immunoprecipitation with anti-APC Ab58 (ALi 12-28), (product ab58), and need to know what class the light chain is for this immunoglobulin--either Kappa I, Kappa II, or Kappa V.

      Read More

      Abcam community

      Verified customer

      Asked on Feb 28 2005

      Answer

      Thank you for your enquiry. I have enquired with the originator of ab58 and unfortunately, we do not have this information. If you have any additional questions, please let us know.

      Read More

      Abcam Scientific Support

      回复于 Mar 10 2005

      Question

      What size band is expected on a Western blot? What dilution should be used?

      Read More

      Abcam community

      Verified customer

      Asked on Jul 20 2004

      Answer

      Thank you for your enquiry. As can be seen in the following publication, this antibody blots wildtype APC at 310kDa. Efstathiou JA et al. 1998. Proc. Natl. Acad. Sci. USA. 95(6):3122-719. I would recommend starting at a dilution of 1:500/1:1000 and optimizing from there. Please also refer to the reviews tab of the online datasheet where customers have reported how the antibody worked for them in Western blotting. If you have any more questions, please contact us again.

      Read More

      Abcam Scientific Support

      回复于 Jul 28 2004

      Question

      For the APC ab 58 , in your protocol you mention 3% BSA/PBS ? Does this mean BSA or PBS or a solution containing both ?

      Read More

      Abcam community

      Verified customer

      Asked on Jan 23 2002

      Answer

      I assume that you are refering to our general FACs protocol where point 2. advises 2. Add 0.1-10 µg/ml of the primary antibody. Dilutions, if necessary, should be made in 3% BSA/PBS Here we mean 3% BSA in 1 X PBS

      Read More

      Abcam Scientific Support

      回复于 Jan 24 2002

      Question

      I have the APC 12-28 0.5ug/ul concentration , and would like to use it for immunoflourescence on fixed slides. What dilution should I use ?

      Read More

      Abcam community

      Verified customer

      Asked on Jan 23 2002

      Answer

      Try this antibody at 1:100 as a starting point and then adjust the dilution according to the results obtained. The actual concentration to use an antibody at is always best assessed by the individual laboratory.

      Read More

      Abcam Scientific Support

      回复于 Jan 24 2002

      Question

      I got a stock of an Ali 12-18 antibody against APC (not 12-28). I couldnt find any information on it on your homepage. On the abcam label on the tube it says that the concentration is 1 ug/ml.is that right??( do you sell this antibody at this concentration)?? I wonder at what concentration I can use this antibody in a western blot analysis. hope you can help with these questions. Thanks a lot

      Read More

      Abcam community

      Verified customer

      Asked on Jun 14 2001

      Answer

      There is a misprint (error) on the tube label - the concentration is actually 1mg/ml. For Western blotting, it is routinely used at 1/1000 dilution although you may have to titrate to optimise for your specific conditions.

      Read More

      Neil Hayward

      Abcam Scientific Support

      回复于 Sep 17 2001

      Question

      Hi. In the datasheet, it is stated that this antibody (anti N-terminal) can be used for histology. I would like to know if this includes use of this antibody in immunohistochemistry using paraffin section. Thank you.

      Read More

      Abcam community

      Verified customer

      Asked on Jun 29 2001

      Answer

      This antibody has not been tested on paraffin sections.

      Read More

      Neil Hayward

      Abcam Scientific Support

      回复于 Jul 03 2001

      Question

      Comments concerning APC-ALi 12-28: 1. enquiry concerning cross reactivity of mAb with non-activated PC: APC means adenomatous polyposis coli not activated protein C in this case 2. experiences with western blot analysis using the mAb on HT29 lysates: unfortunately HT29 does not express a wild type APC protein, both allelic copies harbour truncating mutations

      Read More

      Abcam community

      Verified customer

      Asked on Apr 24 2001

      Answer

      Thank you for your useful comments. We have updated the datasheet to include the full name of the antigen, to avoid confusion with other proteins referred to as APC.

      Read More

      Abcam Scientific Support

      回复于 Apr 24 2001

      Question

      I have read the datasheet and anonymous review of your APC antibody as provided on the web site. Do you have any information as to what dilution the antibody should be used at on Western Blots? Thanks.

      Read More

      Abcam community

      Verified customer

      Asked on Apr 23 2001

      Answer

      Routinely it is used at 1ug per ml although you may have to titrate to find the optimal dilution for your experiment.

      Read More

      Abcam Scientific Support

      回复于 Apr 24 2001

      1-10 of 12 Abreviews or Q&A

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