Anti-APAF1抗体(ab2001)
Key features and details
- Rabbit polyclonal to APAF1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-APAF1抗体
参阅全部 APAF1 一抗 -
描述
兔多克隆抗体to APAF1 -
宿主
Rabbit -
Tested Applications & Species
Application Species IHC-P HumanWB Human -
免疫原
Synthetic peptide corresponding to Human APAF1 aa 12-28 (N terminal).
Sequence:HREALEKDIKTSYIMDHC
(Peptide available asab7873) -
常规说明
Apoptosis Protease Activating Factor.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2001 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
应用 | Species |
---|---|
IHC-P |
Human
|
WB |
Human
|
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
Use a concentration of 1 µg/ml.
|
|
WB | (3) |
1/1000 - 1/2000. Detects a band of approximately 141 kDa.
|
说明 |
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IHC-P
Use a concentration of 1 µg/ml. |
WB
1/1000 - 1/2000. Detects a band of approximately 141 kDa. |
靶标
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功能
Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. -
组织特异性
Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver. -
序列相似性
Contains 1 CARD domain.
Contains 1 NB-ARC domain.
Contains 13 WD repeats. -
结构域
The CARD domain mediates interaction with APIP. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 317 Human
- Omim: 602233 Human
- SwissProt: O14727 Human
- Unigene: 552567 Human
- Unigene: 728891 Human
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别名
- APAF 1 antibody
- Apaf-1 antibody
- APAF_HUMAN antibody
see all
图片
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All lanes : Anti-APAF1 antibody (ab2001) at 1 µg/ml
Lane 1 : K562 cell lysate in absence of blocking peptide
Lane 2 : K562 cell lysate in presence of blocking peptide.
Observed band size: 141 kDa why is the actual band size different from the predicted? -
ab2001 at 1µg/ml staining Apaf1 in human heart tissue by IHC
数据表及文件
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SDS download
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Datasheet download
文献 (20)
ab2001 被引用在 20 文献中.
- C de S L Oliveira AL et al. Effect of Oxaliplatin-Loaded Poly (d,l-Lactide-co-Glycolic Acid) (PLGA) Nanoparticles Combined with Retinoic Acid and Cholesterol on Apoptosis, Drug Resistance, and Metastasis Factors of Colorectal Cancer. Pharmaceutics 12:N/A (2020). PubMed: 32102251
- Guo J et al. MicroRNA-770-5p contributes to podocyte injury via targeting E2F3 in diabetic nephropathy. Braz J Med Biol Res 53:e9360 (2020). PubMed: 32696822
- Mei LH et al. Hyperbaric Oxygen Combined with 5-Aminolevulinic Acid Photodynamic Therapy Inhibited Human Squamous Cell Proliferation. Biol Pharm Bull 42:394-400 (2019). PubMed: 30587670
- Miao J et al. Hydroxytyrosol protects against myocardial ischemia reperfusion injury by inhibiting mitochondrial permeability transition pore opening. Exp Ther Med 17:671-678 (2019). PubMed: 30651849
- Fang H et al. miR-937 regulates the proliferation and apoptosis via targeting APAF1 in breast cancer. Onco Targets Ther 12:5687-5699 (2019). PubMed: 31410016