Key features and details
- Rabbit polyclonal to APAF1
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
参阅全部 APAF1 一抗
经测试应用适用于: IHC-P, ICC/IF, WBmore details
种属反应性与反应: Mouse, Rat, Human
- HeLa whole cell lysate. This antibody gave a positive result in IF/ICC when used in the following formaldehyde fixed cell lines: HeLa.
常规说明Apoptosis Protease Activating Factor.
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
存储溶液Preservative: 0.02% Sodium azide
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab2001 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 µg/ml.|
|ICC/IF||Use a concentration of 10 µg/ml.|
|WB||1/1000 - 1/2000. Detects a band of approximately 141 kDa.|
功能Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.
组织特异性Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.
序列相似性Contains 1 CARD domain.
Contains 1 NB-ARC domain.
Contains 13 WD repeats.
结构域The CARD domain mediates interaction with APIP.
- Information by UniProt
- APAF 1 antibody
- Apaf-1 antibody
- APAF_HUMAN antibody
All lanes : Anti-APAF1 antibody (ab2001) at 1 µg/ml
Lane 1 : K562 cell lysate in absence of blocking peptide
Lane 2 : K562 cell lysate in presence of blocking peptide.
Observed band size: 141 kDa why is the actual band size different from the predicted?
ab2001 at 1µg/ml staining Apaf1 in human heart tissue by IHC
ICC/IF image of ab2001 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab2001 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab2001 被引用在 18 文献中.
- Miao J et al. Hydroxytyrosol protects against myocardial ischemia reperfusion injury by inhibiting mitochondrial permeability transition pore opening. Exp Ther Med 17:671-678 (2019). PubMed: 30651849
- Fang H et al. miR-937 regulates the proliferation and apoptosis via targeting APAF1 in breast cancer. Onco Targets Ther 12:5687-5699 (2019). PubMed: 31410016
- Wu B et al. Combination of Sodium Selenite and Doxorubicin Prodrug Ac-Phe-Lys-PABC-ADM Affects Gastric Cancer Cell Apoptosis in Xenografted Mice. Biomed Res Int 2019:2486783 (2019). PubMed: 31531348
- Zou L et al. Bone marrow mesenchymal stem cell-derived exosomes protect against myocardial infarction by promoting autophagy. Exp Ther Med 18:2574-2582 (2019). PubMed: 31555366
- Wang W et al. miR-222 induces apoptosis in human intervertebral disc nucleus pulposus cells by targeting Bcl-2. Mol Med Rep 20:4875-4882 (2019). PubMed: 31638197