Anti-APAF1抗体(ab2000)
Key features and details
- Rabbit polyclonal to APAF1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-APAF1抗体
参阅全部 APAF1 一抗 -
描述
兔多克隆抗体to APAF1 -
宿主
Rabbit -
Tested Applications & Species
Application Species IHC-P HumanWB Human -
免疫原
Synthetic peptide corresponding to Human APAF1 aa 1158-1177 (C terminal).
Sequence:TFYTNGTNLKKIHVSPDFKT
(Peptide available asab7872) -
常规说明
Apoptosis Protease Activating FactorThe Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2000 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
应用 | Species |
---|---|
IHC-P |
Human
|
WB |
Human
|
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | (1) |
Use a concentration of 20 µg/ml.
|
WB | (1) |
1/500 - 1/2000. Predicted molecular weight: 130 kDa.
|
说明 |
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IHC-P
Use a concentration of 20 µg/ml. |
WB
1/500 - 1/2000. Predicted molecular weight: 130 kDa. |
靶标
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功能
Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. -
组织特异性
Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver. -
序列相似性
Contains 1 CARD domain.
Contains 1 NB-ARC domain.
Contains 13 WD repeats. -
结构域
The CARD domain mediates interaction with APIP. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 317 Human
- Omim: 602233 Human
- SwissProt: O14727 Human
- Unigene: 552567 Human
- Unigene: 728891 Human
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别名
- APAF 1 antibody
- Apaf-1 antibody
- APAF_HUMAN antibody
see all
图片
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All lanes : Anti-APAF1 antibody (ab2000) at 1 µg/ml
Lane 1 : Human heart tissue lysate with absence of blocking peptide
Lane 2 : Human heart tissue lysate with presence of blocking peptide
Predicted band size: 130 kDa
Observed band size: 130 kDa -
Immunohistochemistry of Apaf1 in human skin tissue with Apaf1 antibody at 20 µg/ml.
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab2000 被引用在 7 文献中.
- Zhang X et al. The effects of umbilical cord-derived macrophage exosomes loaded with cisplatin on the growth and drug resistance of ovarian cancer cells. Drug Dev Ind Pharm N/A:1-13 (2020). PubMed: 32482115
- Pan Z et al. Cinobufagin Induces Cell Cycle Arrest at the G2/M Phase and Promotes Apoptosis in Malignant Melanoma Cells. Front Oncol 9:853 (2019). PubMed: 31552178
- Wang SQ et al. Knockdown of IGF-1R Triggers Viral RNA Sensor MDA5- and RIG-I-Mediated Mitochondrial Apoptosis in Colonic Cancer Cells. Mol Ther Nucleic Acids 16:105-117 (2019). PubMed: 30861413
- Liu Y et al. Breviscapine ameliorates CCl4-induced liver injury in mice through inhibiting inflammatory apoptotic response and ROS generation. Int J Mol Med 42:755-768 (2018). PubMed: 29717768
- Gulic T et al. Granulysin-mediated apoptosis of trophoblasts in blighted ovum and missed abortion. Am J Reprod Immunol 80:e12978 (2018). PubMed: 29774968
- Hathaway QA et al. Maternal-engineered nanomaterial exposure disrupts progeny cardiac function and bioenergetics. Am J Physiol Heart Circ Physiol 312:H446-H458 (2017). PubMed: 28011589
- Nichols CE et al. Cardiac and mitochondrial dysfunction following acute pulmonary exposure to mountaintop removal mining particulate matter. Am J Physiol Heart Circ Physiol 309:H2017-30 (2015). PubMed: 26497962