Key features and details
- Goat polyclonal to AP-2 complex subunit alpha-1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
产品名称Anti-AP-2 complex subunit alpha-1抗体
参阅全部 AP-2 complex subunit alpha-1 一抗
描述山羊多克隆抗体to AP-2 complex subunit alpha-1
特异性The immunogen sequence is found in both AP2A2 and AP2A1. This antibody recognises a band of just under 100kD in multiple human cell lines (see picture), the band can be blocked with the immunising peptide.
经测试应用适用于: WBmore details
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab3707 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Detects a band of approximately 100 kDa (predicted molecular weight: 104 , 108 kDa).|
功能Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif.
组织特异性Isoform A expressed in forebrain, skeletal muscle, spinal cord, cerebellum, salivary gland, heart and colon. Isoform B is widely expressed in tissues and also in breast cancer and in prostate carcinoma cells.
序列相似性Belongs to the adaptor complexes large subunit family.
细胞定位Cell membrane. Membrane > coated pit. AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV.
- Information by UniProt
- 100 kDa coated vesicle protein A antibody
- Adapter-related protein complex 2 alpha-1 subunit antibody
- adapter-related protein complex 2 subunit alpha-1 antibody
Western blot using ab3707 at 1/500.
Lane 1: HeLa Nuclear Extract
Lane 2: HeLa Whole Cell Lysate
Lane 3: 293 Whole Cell Lysate
Lane 4: A431 Whole Cell Lysate
Lane 5: Jurkat Whole Cell Lysate
Lane 6: HeLa Nuclear Extract + blocking/immunising peptide
Lane 7: HeLa Whole Cell Lysate + blocking/immunising peptide
Lane 8: 293 Whole Cell Lysate + blocking/immunising peptide
Lane 9: A431 Whole Cell Lysate + blocking/immunising peptide
Lane 10: Jurkat Whole Cell Lysate + blocking/immunising peptide
AP2 alpha proteins (AP2A2 and AP2A1) have predicted molecular weights of 104 and 107kD. The band at just below 97kD (that is blocked by the immunising peptide) represents AP2 alpha, the lower bands are not blocked completely by the immunising peptide and are believed to be non-specific.
Secondary ab: Rabbit polyclonal to Goat IgG HRP
Exposure time: 1 minute.<