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Kits/ Lysates/ Other Tools and Reagents IHC Tools/ Reagents

Antigen Retrieval缓冲液(100X Tris-EDTA缓冲液,pH 9.0) (ab93684)

  • Datasheet
  • SDS
Reviews (2)Q&A (2)References (5)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

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概述

  • 产品名称

    Antigen Retrieval缓冲液(100X Tris-EDTA缓冲液,pH 9.0)
  • 经测试应用

    适用于: IHC-Pmore details
  • 常规说明

    Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) enables target retrieval in formalin-fixed, paraffin-embedded tissue sections in one step. It is optimal for use with primary antibodies that require Tris-EDTA buffer (pH 9.0) pretreatment.

    This product contains detergent for emulsification of the paraffin.

    1X Dilution: The 100X stock solution should be diluted 100-fold with distilled water before use.

    Protocol:

    1. Place paraffin-embedded slides in 1x Antigen Retrieval Buffer; cover with a vented plastic wrap, place in microwave and set high power to boil and then set low power to keep it boiling for 10 min. Let the sections cool in the microwave for at least 20min.
    2. Wash sections with hot tap water for 1 minute.
    3. Wash sections in buffer for 2x3 minutes.
    4. Continue with IHC protocol 

    Other kits and reagents for IHC

    Other antigen retrieval buffers include: Citrate buffer pH 6.0 ab93678, EDTA buffer pH 8.0 ab93680, Tris buffer pH 10.0 ab93682, or see the full list of antigen retrieval buffers and enzymes.

    Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

性能

  • 形式

    Liquid
  • 存放说明

    Store at room temperature.
  • 存储溶液

    pH: 8.7
    Constituents: 1.5% 2-butoxyethanol, 5% Sodium EDTA, 5% Tris

    Buffer 100x concentrated with detergent.
  • Concentration information loading...
  • 研究领域

    • Kits/ Lysates/ Other
    • Tools and Reagents
    • IHC Tools/ Reagents
    • Kits/ Lysates/ Other
    • Kits
    • IHC tools
    • Antigen retrieval reagent
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相关产品

    应用

    Our Abpromise guarantee covers the use of ab93684 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    IHC-P Use at an assay dependent dilution.

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)
      ab93684 Antigen Retrieval Buffer 100X Tris-EDTA Buffer, pH 9
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

      Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling LINE-1 ORF1p with ab216324 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on spermatogonia of mouse testis (PMID: 24607009) is observed. Counterstained with hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
      Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    数据表及文件

    • Datasheet
    • SDS
  • 文献 (5)

    发表研究结果有使用 ab93684?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab93684 被引用在 5 文献中.

    • Celik B  et al. Tissue HE4 Expression Discriminates the Ovarian Serous Carcinoma but Not the Uterine Serous Carcinoma Patients. A New Adjunct to the Origin of the Tumor Site. Pathol Oncol Res 26:1145-1151 (2020). PubMed: 31165997
    • Xue XY  et al. The full transcription map of mouse papillomavirus type 1 (MmuPV1) in mouse wart tissues. PLoS Pathog 13:e1006715 (2017). PubMed: 29176795
    • Gross C  et al. Osteoclast profile of medication-related osteonecrosis of the jaw secondary to bisphosphonate therapy: a comparison with osteoradionecrosis and osteomyelitis. J Transl Med 15:128 (2017). PubMed: 28587628
    • Celik B & Bulut T Human epididymis protein 4 may not be a reliable screening biomarker for detecting lung carcinoma patients. Biomed Rep 7:297-300 (2017). PubMed: 29085624
    • Li Y  et al. Perinatal nicotine exposure increases vulnerability of hypoxic-ischemic brain injury in neonatal rats: role of angiotensin II receptors. Stroke 43:2483-90 (2012). PubMed: 22738920

    客户评价及客户问答

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    1-4 of 4 Abreviews or Q&A

    antigene retrieval buffer

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    abreview image
    The reagent works well. We use it in concentration 1x in steam pressure pot on paraffin samples.

    Dr. Jeeho Kim

    Verified customer

    提交于 Dec 30 2019

    Tris-EDTA Buffer Antigen Retrieval

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    The reagent works well. We use it in concentration 1x in steam pressure pot on paraffin samples.
    The buffer is necessary for these antibodies that require a pH 9.0 HIER demasking.

    Abcam user community

    Verified customer

    提交于 Sep 02 2019

    Question

     I would like to inform you that I plan to use Peroxide/DAB the substrate and chromogen for horseradish peroxidase as detection system I appreciate you for your kind help                                                       with my best regards                                                    

    Read More

    Abcam community

    Verified customer

    Asked on Oct 11 2012

    Answer

    Thank you for your reply. I recommend the following HRP conjugated secondary antibodies to use with these rabbit Anti-GAD65+GAD67 primary antibodies. I have included one product from a donkey host and another from goat. Both are guaranteed and the choice will be personal preference of host. These products are below. Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) Ab16284: https://www.abcam.com/Rabbit-IgG-secondary-antibody-ab16284.html Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) Ab97051: https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-HL-HRP-ab97051.html Abcam also provides a number of IHC reagents and kits such as DAB substrate kits, donkey or goat sera and antigen retrieval buffers. All of our products are covered by our six month Abpromise guarantee and are fully supported by our scientific support staff. I have provided links to a few of these products below. DAB substrate kit:https://www.abcam.com/DAB-substrate-kit-ab94665.html Donkey serum:https://www.abcam.com/Donkey-Serum-ab7475.html Goat serum:https://www.abcam.com/Goat-Serum-ab7481.html Antigen retrieval buffer:https://www.abcam.com/Antigen-Retrieval-Buffer-100X-Tris-EDTA-Buffer-pH-9-0-ab93684.html I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. Use our products? Submit an Abreview. Earn rewards! https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    回复于 Oct 11 2012

    Question

    What protocol do you use with this product?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 04 2012

    Answer

    Thank you for calling Abcam earlier today.

    Please find the recommended protocol to use with ab93684 attached.

    If there is anything else I can help you with, please let me know.

    Read More

    Abcam Scientific Support

    回复于 Oct 04 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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