产品名称Antigen Retrieval缓冲液(100X EDTA缓冲液，pH 8.0)
经测试应用适用于: IHC-Pmore details
Antigen Retrieval Buffer (100X EDTA Buffer, pH 8.0) is a special solution that enables rehydrate and target retrieval in formalin-fixed, paraffin-embedded tissue sections in one step. It is optimal for use with primary antibodies that require EDTA buffer (pH=8.0) pretreatment.
This product contains detergent for emulsification of the paraffin.
1X Dilution: The 100X stock solution should be diluted 100-fold with distilled water before use.
- Place paraffin-embedded slides in 1x Antigen Retrieval Buffer; cover with a vented plastic wrap, place in microwave and set high power to boil and then set low power to keep it boiling for 10 min. Let the sections cool in the microwave for at least 20min.
- Wash sections with hot tap water for 1 minute.
- Wash sections in buffer for 2x3 minutes.
- Continue with IHC protocol as normal.
Other kits and reagents for IHC
Other antigen retrieval buffers include:
Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
存放说明Store at room temperature.
Constituents: 1.5% 2-butoxyethanol, 5% Sodium EDTA
Note: Buffer 100x Concentrated with detergent.
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Our Abpromise guarantee covers the use of ab93680 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent dilution.|