概述

  • 产品名称

    Antigen Retrieval缓冲液(100X EDTA缓冲液,pH 8.0)
  • 经测试应用

    适用于: IHC-Pmore details
  • 常规说明

    Antigen Retrieval Buffer (100X EDTA Buffer, pH 8.0) is a special solution that enables rehydrate and target retrieval in formalin-fixed, paraffin-embedded tissue sections in one step. It is optimal for use with primary antibodies that require EDTA buffer (pH=8.0) pretreatment.

    This product contains detergent for emulsification of the paraffin.

    1X Dilution: The 100X stock solution should be diluted 100-fold with distilled water before use.

    PROTOCOL:

    1. Place paraffin-embedded slides in 1x Antigen Retrieval Buffer; cover with a vented plastic wrap, place in microwave and set high power to boil and then set low power to keep it boiling for 10 min. Let the sections cool in the microwave for at least 20min.
    2. Wash sections with hot tap water for 1 minute.
    3. Wash sections in buffer for 2x3 minutes.
    4. Continue with IHC protocol as normal.

    Other kits and reagents for IHC

    Other antigen retrieval buffers include:

    Citrate buffer pH 6.0 ab93678, Tris-EDTA buffer pH 9.0 ab93684Tris buffer pH 10.0 ab93682, or see the full list of antigen retrieval buffers and enzymes.

    Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

性能

应用

Our Abpromise guarantee covers the use of ab93680 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent dilution.

图片

  • ab93680 Antigen Retrieval Buffer 100X EDTA Buffer, pH 8

文献

This product has been referenced in:

  • Huang CW  et al. Ophiocordyceps formosana improves hyperglycemia and depression-like behavior in an STZ-induced diabetic mouse model. BMC Complement Altern Med 16:310 (2016). IHC-P . Read more (PubMed: 27553852) »
See 1 Publication for this product

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