Key features and details
- Rabbit polyclonal to ANP32E
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
参阅全部 ANP32E 一抗
特异性This antibody does not cross-react with PHAPI or PHAP12a.
经测试应用适用于: ICC/IF, WB, IHC-Pmore details
种属反应性与反应: Mouse, Rat, Human
Synthetic peptide corresponding to Human ANP32E (C terminal).
(Peptide available as
- A549 or HepG2 whole cell lysate (ab7900).
This product was previously labelled as LANPL
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab5993 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 35 kDa.|
|IHC-P||Use a concentration of 2 µg/ml.|
功能Inhibits activity of protein phosphatase 2A (PP2A). Does not inhibit protein phosphatase 1. May play a role in cerebellar development and synaptogenesis process by modulating PP2A activity.
组织特异性Expressed in peripheral blood leukocytes, colon, small intestine, prostate, thymus, spleen, skeletal muscle, liver and kidney.
序列相似性Belongs to the ANP32 family.
Contains 4 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
翻译后修饰Phosphorylated. The phosphorylation is nuclear localization signal (NLS)-dependent.
- Information by UniProt
- Acidic (leucine rich) nuclear phosphoprotein 32 family member E antibody
- Acidic leucine-rich nuclear phosphoprotein 32 family member E antibody
- AN32E_HUMAN antibody
Western blot analysis of PHAP III expression in human A549 (A) and HepG2 (B) cells, and rat testis (C) with anti-PHAP III at 1 µg/ml. Western blot analysis of ANP32E expression in human A549 (A) and HepG2 (B) cells, and rat testis (C) with ab5993 at 1 µg/ml.
ab5993 at 2µg/ml staining ANP32E in human small intestine tissue by IHC
ICC/IF image of ab5993 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5993, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunofluorescence of PHAP III in Human Small Intestine cells using ab5993 at 20 ug/ml.
ab5993 被引用在 3 文献中.
- Huang J et al. Up-regulated ANP32E promotes the thyroid carcinoma cell proliferation and migration via activating AKT/mTOR/HK2-mediated glycolysis. Gene 750:144681 (2020). PubMed: 32304784
- Shin H et al. Transcriptional regulation mediated by H2A.Z via ANP32e-dependent inhibition of protein phosphatase 2A. Biochim Biophys Acta 1861:481-496 (2018). ChIP-sequencing ; Mouse . PubMed: 29524612
- Mao Z et al. Anp32e, a higher eukaryotic histone chaperone directs preferential recognition for H2A.Z. Cell Res 24:389-99 (2014). Human . PubMed: 24613878