Annexin V/ANXA5-Cy5 Apoptosis Staining / Detection试剂(ab14147)

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概述

  • 产品名称

    Annexin V/ANXA5-Cy5 Apoptosis Staining / Detection试剂
    参阅全部 Annexin V/ANXA5 试剂

  • 经测试应用

    适用于: FM, Flow Cytmore details

  • 常规说明

    Annexin V/ANXA5-Cy5 Apoptosis Detection Reagent ab14147 can be used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane. Analysis is by flow cytometry or fluorescence microscopy.

    This Annexin V/ANXA5-Cy5 reagent can be used with Annexin V/ANXA5 binding buffer ab14084 (see protocol section for instructions). The Annexin V/ANXA5-Cy5 reagent is also available as part of Annexin V/ANXA5-Cy5 apoptosis kit ab14150.

    For more apoptosis assays, review the full set of Annexin V/ANXA5 assays, or the apoptosis assay and apoptosis marker guide.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab14147于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
FM
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent dilution.
说明
FM
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent dilution.

文献 (2)

发表研究结果有使用 ab14147？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab14147 被引用在 2 文献中.

  • Guo X  et al. Global characterization of T cells in non-small-cell lung cancer by single-cell sequencing. Nat Med 24:978-985 (2018). PubMed: 29942094
  • Wu KW  et al. Neurovascular Interaction Promotes the Morphological and Functional Maturation of Cortical Neurons. Front Cell Neurosci 11:290 (2017). PubMed: 28966577

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1-3 of 3 Abreviews or Q&A

Question

Inquiry: Hello, I amm testing Cy5-Annexin-V reagents. Can you please tell me whether the annexin used in the preparation of this product is a recombinant protein? Some vendors provide material isolated from human placenta and some use E.Coli generated material. Many thanks,

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Answer

Thank you for contacting us.

The source is E.Coli and it is a recombinant Annexin V.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

Hi,   I recently purchased Annexin V-Cy5 ab14147 from you and had a question that wasn’t covered in the pack literature that came with it.   What is the source of Annexin (i.e. Human, recombinant etc.)? Regards,

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Answer

Thank you for contacting us. The source is E.Coli and it is a recombinant Annexin V. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. Use our products? Submit an Abreview. Earn rewards! https://www.abcam.com/abreviews

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Question

Good day     Please provide us with a Certificate of Analysis and a Certificate of Origin for :   AB14147 Lot # GR88608-1

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Answer

Thank you for your enquiry.Some of our antibodies are produced in-house and others are sourced from suppliers or academic collaborators whose products we believe will be of interest to our customers. All these products are guaranteed by our Abpromise to be of high quality. Our Abpromise assures you that if our products do not perform as our datasheets say they should we offer a replacement or refund within six month of purchase. Some of our collaborators are not certified ISO9001 and so we are not able to provide a certificate of analysis for these particular products.As the identity of our collaborators is confidential, we provide a certificate of compliance for all of our products available in our catalogue. This document certifies that our products meet the required specifications for a particular lot/batch of product.

I am happy to confirm that I attached the CoC to this email.

As for the "Certificate of origin", I regret to inform you that we do not provide it, because in addition to our in-house products, there are some products which are supplied to us from outside collaborators, just as in the case of Annexin V-Cy5 Apoptosis Detection Reagentab14147. I can however confirm that we do source is from a collaborating laboratory in the USA.

Please do not hesitate to contact me again if you have further questions.

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