Annexin V-CF Blue 7-AAD Apoptosis Staining / Detection试剂盒(ab214663)
Key features and details
- Assay type: Cell-based (quantitative)
- Detection method: Fluorescent
- Platform: Flow cytometer
- Sample type: Adherent cells, Suspension cells
概述
-
产品名称
Annexin V-CF Blue 7-AAD Apoptosis Staining / Detection试剂盒
参阅全部 Annexin V/ANXA5 试剂盒 -
检测方法
Fluorescent -
样品类型
Adherent cells, Suspension cells -
检测类型
Cell-based (quantitative) -
种属反应性
与反应: Mammals, Other species -
产品概述
Annexin V-CF Blue 7-AAD Apoptosis Staining / Detection Kit (ab214663) contains Annexin V labeled with CF Blue, which allows the identification and quantitation of apoptotic cells on a single-cell basis by flow cytometry. Simultaneous staining of cells with Annexin V–CF Blue (blue fluorescence) and the non-vital dye 7-aminoactinomycin D (7-AAD) (red fluorescence) allows the discrimination of intact cells (Annexin V-CF Blue negative, 7-AAD Staining Solution negative), early apoptotic (Annexin V-CF Blue positive, 7-AAD Staining Solution negative) and late apoptotic or necrotic cells (Annexin V-CF Blue positive, 7-AAD Staining Solution positive).
CF-Blue can be used as direct replacement for Pacific Blue™ or similar blue dyes.
For more apoptosis assays, review the full set of Annexin V assays, or the apoptosis assay and apoptosis marker guide.
-
说明
Exposure of phosphatidylserine (PS) on the external surface of the cell membrane has been reported to occur in the early phases of apoptotic cell death, during which the cell membrane remains intact. In leukocyte apoptosis, PS on the outer surface of the cell marks the cell for recognition and phagocytosis by macrophages. The Annexin V protein has a high affinity for PS, and shows minimal binding to phosphatidylcholine and sphingomyelin. Changes in PS asymmetry, which can be analyzed by measuring annexin V binding to the cell membrane, are generally observed before morphological changes associated with apoptosis occurred and before membrane integrity is lost.
-
平台
Flow cytometer
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
组件 100 tests 10X Binding Buffer Concentrate 1 x 50ml 7-AAD Staining Solution in PBS (pH 7.4) 1 x 500µl Annexin V-CF Blue Conjugate ( PBS, pH 7.2) 1 x 500µl -
研究领域
-
功能
This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. -
疾病相关
Pregnancy loss, recurrent, 3 -
序列相似性
Belongs to the annexin family.
Contains 4 annexin repeats. -
结构域
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
翻译后修饰
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. - Information by UniProt
-
别名
- Anchorin CII
- Annexin 5
- Annexin A5
see all
相关产品
-
Related Products
- FCCP, mitochondrial oxidative phosphorylation uncoupler (ab120081)
- Z-VAD(OH)-FMK, Irreversible general caspase inhibitor (ab120382)
- Doxorubicin hydrochloride, Topoisomerase II inhibitor (ab120629)
- Dorsomorphin (Compound C), AMP-kinase inhibitor (ab120843)
- Annexin V/ANXA5-FITC Apoptosis Detection Reagent (500X) (ab14082)
- Propidium Iodide (ab14083)
- 10X Annexin V/ANXA5 Binding Buffer (ab14084)
- Calcein AM, fluorescent dye for cell viability (ab141420)
- Annexin V/ANXA5-Cy3 Apoptosis Staining / Detection Reagent (ab14143)
- Annexin V-Cy3 Apoptosis Staining / Detection Kit with SYTOX (ab14144)
- Annexin V/ANXA5-Cy5 Apoptosis Staining / Detection Reagent (ab14147)
- Annexin V-Cy5 Apoptosis Staining / Detection Kit (ab14150)
- Annexin V-EGFP Apoptosis Staining / Detection Kit (ab14153)
- Annexin V/ANXA5-PE Apoptosis Staining / Detection Reagent (ab14154)
- Annexin V- DY-634 PI Apoptosis Staining / Detection Kit (ab214484)
- Annexin V-CF Blue PI Apoptosis Staining / Detection Kit (ab214485)
图片
数据表及文件
-
SDS download
-
Datasheet download
文献 (11)
ab214663 被引用在 11 文献中.
- Kim D et al. Autologous treatment for ALS with implication for broad neuroprotection. Transl Neurodegener 11:16 (2022). PubMed: 35272709
- Pei J et al. Progesterone Attenuates SIRT1-Deficiency-Mediated Pre-Eclampsia. Biomolecules 12:N/A (2022). PubMed: 35327614
- Ruiz-Rodado V et al. Cysteine is a limiting factor for glioma proliferation and survival. Mol Oncol 16:1777-1794 (2022). PubMed: 34856072
- Alsherbiny MA et al. Metabolomic Identification of Anticancer Metabolites of Australian Propolis and Proteomic Elucidation of Its Synergistic Mechanisms with Doxorubicin in the MCF7 Cells. Int J Mol Sci 22:N/A (2021). PubMed: 34360606
- Alsherbiny MA et al. Synergistic Interactions of Cannabidiol with Chemotherapeutic Drugs in MCF7 Cells: Mode of Interaction and Proteomics Analysis of Mechanisms. Int J Mol Sci 22:N/A (2021). PubMed: 34576262