Anti-ACE2抗体(ab15348)
Key features and details
- Rabbit polyclonal to ACE2
- Suitable for: ICC/IF, ELISA, IHC (PFA fixed), IHC-Fr, WB, IHC-P
- Reacts with: Mouse, Rat, Cat, Human, Ferret, Macaque monkey, Bat
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-ACE2抗体
参阅全部 ACE2 一抗 -
描述
兔多克隆抗体to ACE2 -
宿主
Rabbit -
特异性
No cross reactivity to ACE1.
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经测试应用
适用于: ICC/IF, ELISA, IHC (PFA fixed), IHC-Fr, WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Cat, Human, Ferret, Macaque monkey, Bat -
免疫原
Synthetic peptide within Human ACE2 aa 750 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q9BYF1 -
阳性对照
- WB: Human testis, lung, intestine, breast and kidney tissue lysates. Caco2 cell lysate. Rat skin, spleen, heart and brain tissue lysates. Mouse liver, spleen, heart, bladder, pancreas and stomach tissue lysates. IHC-P: Human lung, testis and kidney tissue. Rat lung tissue. Mouse lung tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading...
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纯度
Immunogen affinity purified -
Primary antibody说明
No cross reactivity to ACE1. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab15348于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF | (2) |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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IHC (PFA fixed) |
Use at an assay dependent concentration.
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IHC-Fr | (1) |
Use at an assay dependent concentration.
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WB | (5) |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 97 kDa).
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IHC-P | (5) |
Use a concentration of 2 µg/ml.
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说明 |
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ICC/IF
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
IHC (PFA fixed)
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 97 kDa). |
IHC-P
Use a concentration of 2 µg/ml. |
靶标
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功能
Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses. -
组织特异性
Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system. -
序列相似性
Belongs to the peptidase M2 family. -
翻译后修饰
N-glycosylation on Asn-90 may limit SARS infectivity. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 59272 Human
- Entrez Gene: 70008 Mouse
- Entrez Gene: 302668 Rat
- Omim: 300335 Human
- SwissProt: Q9BYF1 Human
- SwissProt: Q8R0I0 Mouse
- SwissProt: Q5EGZ1 Rat
- Unigene: 178098 Human
see all -
别名
- ACE 2 antibody
- ACE related carboxypeptidase antibody
- ACE-related carboxypeptidase antibody
see all
图片
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All lanes : Anti-ACE2 antibody (ab15348) at 1/1000 dilution
Lane 1 : Wild-type Caco-2 cell lysate
Lane 2 : ACE2 knockout Caco-2 cell lysate
Lane 3 : Calu-3 cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 30 µg per lane.
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 120-135 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-ACE2 antibody staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab15348 was shown to bind specifically to ACE2. A band was observed at 120-135 kDa in wild-type Caco-2 cell lysates with no signal observed at this size in ACE2 knockout cell line ab277338 (knockout cell lysate ab275516). To generate this image, wild-type and ACE2 knockout Caco-2 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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All lanes : Anti-ACE2 antibody (ab15348) at 2 µg/ml
Lane 1 : Human Testis Lysate
Lane 2 : Human Lung lysate
Lane 3 : Human intestine lysate
Lane 4 : Human Breast lysate
Lane 5 : Caco2 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 97 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labeling ACE2 with ab15348 at 20 μg/mL.
Goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labeling ACE2 with ab15348 at 20 μg/mL.
Goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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All lanes : Anti-ACE2 antibody (ab15348) at 2 µg/ml
Lane 1 : Rat skin tissue lysate
Lane 2 : Rat spleen tissue lysate
Lane 3 : Rat heart tissue lysate
Lane 4 : Rat brain tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 97 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?Western blot of ab15348 incubated for 1h at RT in 5% NFDM/TBST.
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All lanes : Anti-ACE2 antibody (ab15348) at 2 µg/ml
Lane 1 : Mouse liver tissue lysate
Lane 2 : Mouse spleen tissue lysate
Lane 3 : Mouse heart tissue lysate
Lane 4 : Mouse bladder tissue lysate
Lane 5 : Mouse pancreas tissue lysate
Lane 6 : Mouse stomach tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 97 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?Western blot of ab15348 incubated for 1h at RT in 5% NFDM/TBST.
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Lane 1 : Anti-ACE2 antibody (ab15348) at 0.5 µg/ml
Lane 2 : Anti-ACE2 antibody (ab15348) at 1 µg/ml
Lane 3 : Anti-ACE2 antibody (ab15348) at 2 µg/ml
All lanes : Human Kidney Lysate
Predicted band size: 97 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?Western blot analysis of ACE2 in human kidney lysate with ab15438 at 0.5 μg/mL (lane A), 1 μg/mL (lane B), and 2 μg/mL (lane C).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lung tissue labeling ACE2 with ab15348 at 20 μg/mL.
Goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat lung tissue labeling ACE2 with ab15348 at 20 μg/mL.
Goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labeling ACE2 with ab15348 at 2 μg/mL.
Tissue was fixed with formaldehyde and blocked with 10% serum for 1 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG HRP at 1/250 was used as secondary. Counter stained with Hematoxylin.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (192)
ab15348 被引用在 192 文献中.
- Silva MG et al. Effect of age on human ACE2 and ACE2-expressing alveolar type II cells levels. Pediatr Res 93:948-952 (2023). PubMed: 35739259
- Wu CT et al. SARS-CoV-2 replication in airway epithelia requires motile cilia and microvillar reprogramming. Cell 186:112-130.e20 (2023). PubMed: 36580912
- Suarez-Meade P et al. SARS-CoV2 entry factors are expressed in primary human glioblastoma and recapitulated in cerebral organoid models. J Neurooncol 161:67-76 (2023). PubMed: 36595192
- Treleaven T et al. Stage-Specific L-Proline Uptake by Amino Acid Transporter Slc6a19/B0AT1 Is Required for Optimal Preimplantation Embryo Development in Mice. Cells 12:N/A (2022). PubMed: 36611813
- Sepe S et al. DNA damage response at telomeres boosts the transcription of SARS-CoV-2 receptor ACE2 during aging. EMBO Rep 23:e53658 (2022). PubMed: 34854526