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Cardiovascular Angiogenesis Inhibitors

Anti-Angiostatin抗体(ab2904)

  • Datasheet
Reviews (2)Q&A (3)References (11)

Product price, shipping and contact information

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Immunocytochemistry/ Immunofluorescence - Anti-Angiostatin antibody (ab2904)
  • Immunocytochemistry/ Immunofluorescence - Anti-Angiostatin antibody (ab2904)
  • Western blot - Anti-Angiostatin antibody (ab2904)

Key features and details

  • Rabbit polyclonal to Angiostatin
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-Angiostatin抗体
  • 描述

    兔多克隆抗体to Angiostatin
  • 宿主

    Rabbit
  • 特异性

    Detects recombinant human angiostatin.
  • 经测试应用

    适用于: WB, ICC/IFmore details
  • 种属反应性

    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Mouse Angiostatin aa 98-116.
    Sequence:

    VYLSECKTGIGNGYRGTMS


    (Peptide available as ab4925)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cardiovascular
    • Angiogenesis
    • Inhibitors
    • Cardiovascular
    • Blood
    • Fibrinolysis / Thrombolysis
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • Angiogenic inhibitory factors

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human Angiostatin protein (ab123771)

应用

Our Abpromise guarantee covers the use of ab2904 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2 µg/ml. Detects a band of approximately 38, 50 kDa (predicted molecular weight: 38 , 50 kDa).
ICC/IF Use at an assay dependent concentration.

靶标

  • 相关性

    Angiostatin is an internal proteolytic fragment of plasminogen, containing at least three Kringle domains. The Kringle domains have specific anti-migratory and anti-proliferative activities. Angiostatin, an endogenous angiogenesis inhibitor, inhibits endothelial cell proliferation in vitro and angiogenesis in vivo. It is a potent and specific natural inhibitor of angiogenisis and metastatic tumor growth.
  • 细胞定位

    Secreted
  • 数据库链接

    • Entrez Gene: 5340 Human
    • Entrez Gene: 18815 Mouse
    • Omim: 173350 Human
    • SwissProt: P00747 Human
    • SwissProt: P20918 Mouse

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-Angiostatin antibody (ab2904)
      Immunocytochemistry/ Immunofluorescence - Anti-Angiostatin antibody (ab2904)

      Immunocytochemistry/Immunofluorescence analysis of Angiostatin (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2904 in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

    • Immunocytochemistry/ Immunofluorescence - Anti-Angiostatin antibody (ab2904)
      Immunocytochemistry/ Immunofluorescence - Anti-Angiostatin antibody (ab2904)

      Immunocytochemistry/Immunofluorescence analysis of Angiostatin (green) showing staining in the cytoplasm of K562 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab2904 in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

    • Western blot - Anti-Angiostatin antibody (ab2904)
      Western blot - Anti-Angiostatin antibody (ab2904)
      All lanes : Anti-Angiostatin antibody (ab2904) at 1 µg/ml

      Lane 1 : Human liver tissue lysate - total protein (ab29889)
      Lane 2 : Human Plasma Total Protein Lysate
      Lane 3 : Human testis tissue lysate - total protein (ab30257)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 38 , 50 kDa
      Observed band size: 38 + 50 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 27 kDa, 48 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 12 minutes

    实验方案

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet
  • 文献 (11)

    发表研究结果有使用 ab2904?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab2904 被引用在 11 文献中.

    • Ghatak S  et al. Role of Integrins a1ß1 and a2ß1 in Wound and Tumor Angiogenesis in Mice. Am J Pathol 186:3011-3027 (2016). PubMed: 27639165
    • Kubo S  et al. Combinatorial anti-angiogenic gene therapy in a human malignant mesothelioma model. Oncol Rep 34:633-8 (2015). PubMed: 26082103
    • Montagner A  et al. Src is activated by the nuclear receptor peroxisome proliferator-activated receptor ß/d in ultraviolet radiation-induced skin cancer. EMBO Mol Med 6:80-98 (2014). IF ; Mouse . PubMed: 24203162
    • Wu T  et al. Urinary angiostatin--a novel putative marker of renal pathology chronicity in lupus nephritis. Mol Cell Proteomics 12:1170-9 (2013). Human . PubMed: 23345539
    • Pushpakumar SB  et al. Folic acid mitigates angiotensin-II-induced blood pressure and renal remodeling. PLoS One 8:e83813 (2013). WB ; Mouse . PubMed: 24386282
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-5 of 5 Abreviews or Q&A

    Western blot abreview for Anti-Angiostatin antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (heart)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    10 µg
    Specification
    heart
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    提交于 Sep 26 2017

    Western blot abreview for Anti-Angiostatin antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (lung)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native)
    Loading amount
    30 µg
    Specification
    lung
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Oct 05 2015

    Question

    We have ordered this Angiostatin Ab (Ab2904), our question is, does this Ab recognize plasminogen?

    Read More

    Abcam community

    Verified customer

    Asked on Sep 18 2012

    Answer

    The antibody recognizes angiostatin: plasminogen (as well as PLC and Microplasmin) are synonyms for angiostatin.

    I hope this answers your question. Please feel free to contact us with any additional questions.

    Read More

    Abcam Scientific Support

    回复于 Sep 18 2012

    Question

    I have a customer who is interested in AB2904 Angiostatin antibody and wants to know if the samples have to be in reducing form or non-reducing? Also what are your recommendations for blocking? (Application is WB)' I am looking forward to your reply.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 02 2009

    Answer

    From the reading of references which have used this antibody I would expect this antibody to have been used in a reduced form as the materials and methods for the following reference state they performed a standard WB (Balaggan KS et al. EIAV vector-mediated delivery of endostatin or angiostatin inhibits angiogenesis and vascular hyperpermeability in experimental CNV. Gene Ther 13:1153-65 (2006). PubMed: 16572190). We also recommend to use 5% Non-fat dry milk to block the membrane and to incubate the primary antibody in TBST with milk as well. Please do not hesitate to contact us if your customer has further questions.

    Read More

    Abcam Scientific Support

    回复于 Feb 02 2009

    Question

    The product's name is Plasminogen antibody but on the specificity it states that it detects recombinant human angistatin - I was wondering if you've done WB and detected these two molecules with this antibody.

    Read More

    Abcam community

    Verified customer

    Asked on Sep 28 2006

    Answer

    Thank you for your enquiry. Regarding the polyclonal antibody to Angiostatin (ab2904), this has not been tested for cross-reactivity with plasminogen at this time. I would assume if the antibody detects angiostatin, it should also bind to plasminogen since angiostatin is a cleavage product of plasminogen. At this time, however, the antibody has only been tested on recombinant samples. I am looking into having this datasheet changed to reflect that the antibody was targeted specifically to angiostatin and not plasminogen. I hope this information helps. Please do not hesitate to contact us again if you need anything further.

    Read More

    Abcam Scientific Support

    回复于 Sep 29 2006

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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