Application
Western blot
Sample
Mouse Tissue lysate - other (white fat tissue)
Gel Running Conditions
Non-reduced Denaturing (12.5%)
Loading amount
30 µg
Specification
white fat tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Other product details
Incubation time
1 hour(s) and 0 minute(s)
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: promega anti-Rabbit Ig HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 50 kDa
Positive control
mice white fat tissue
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jan 22 2014