Anti-Angiopoietin-like 4/ANGPTL4抗体(ab196746)
Key features and details
- Rabbit polyclonal to Angiopoietin-like 4/ANGPTL4
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Angiopoietin-like 4/ANGPTL4抗体
参阅全部 Angiopoietin-like 4/ANGPTL4 一抗 -
描述
兔多克隆抗体to Angiopoietin-like 4/ANGPTL4 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant full length protein corresponding to Human Angiopoietin-like 4/ANGPTL4 (internal sequence).
Database link: Q9BY76 -
阳性对照
- Skeletal Muscle (Mouse) Whole Cell Lysate (ab3869) can be used as a positive control in WB. Mouse skeletal muscle extract; Rat lung and Human esophageal cancer tissues; A549 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS, 0.87% Sodium chloride
PBS without Mg2+ and Ca2+. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab196746于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
1/50 - 1/200.
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IHC-P |
1/50 - 1/200.
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WB |
1/500 - 1/2000. Predicted molecular weight: 45 kDa.
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说明 |
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ICC/IF
1/50 - 1/200. |
IHC-P
1/50 - 1/200. |
WB
1/500 - 1/2000. Predicted molecular weight: 45 kDa. |
靶标
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功能
Protein with hypoxia-induced expression in endothelial cells. May act as a regulator of angiogenesis and modulate tumorgenesis. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. May exert a protective function on endothelial cells through an endocrine action. It is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity. In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM). The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation. -
组织特异性
Expressed at high levels in the placenta, heart, liver, muscle, pancreas and lung but expressed poorly in the brain and kidney. -
序列相似性
Contains 1 fibrinogen C-terminal domain. -
翻译后修饰
N-glycosylated. -
细胞定位
Secreted. Secreted > extracellular space > extracellular matrix. The unprocessed form interacts with the extracellular matrix. This may constitute a dynamic reservoir, a regulatory mechanism of the bioavailability of ANGPTL4. - Information by UniProt
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数据库链接
- Entrez Gene: 51129 Human
- Entrez Gene: 57875 Mouse
- Entrez Gene: 362850 Rat
- Omim: 605910 Human
- SwissProt: Q9BY76 Human
- SwissProt: Q9Z1P8 Mouse
- SwissProt: Q6TMA8 Rat
- Unigene: 9613 Human
see all -
别名
- Angiopoietin like 4 antibody
- Angiopoietin related protein 4 antibody
- Angiopoietin-like protein 4 antibody
see all
图片
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Anti-Angiopoietin-like 4/ANGPTL4 antibody (ab196746) at 1/500 dilution + Mouse skeletal muscle extract
Predicted band size: 45 kDa -
Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling Angiopoietin-like 4/ANGPTL4 with ab196746 at 1/100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human esophageal cancer tissue labeling Angiopoietin-like 4/ANGPTL4 with ab196746 at 1/100 dilution.
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Immunofluorescent analysis of A549 cells labeling Angiopoietin-like 4/ANGPTL4 with ab196746 at 1/50 dilution. Blue: DAPI for nuclear staining.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (17)
ab196746 被引用在 17 文献中.
- Xiang X et al. Oleic and palmitic acids induce hepatic angiopoietin-like 4 expression predominantly via PPAR-γ in Larimichthys crocea. Br J Nutr 129:1657-1666 (2023). PubMed: 34556193
- Kamaludin Z et al. Angiopoietin-Like Protein 4 and Insulin-Like Growth Factor-1 Expression in Invasive Breast Carcinoma in Young Women. Pathophysiology 29:9-23 (2022). PubMed: 35366286
- Zhang K et al. DNA methylation mediated down-regulation of ANGPTL4 promotes colorectal cancer metastasis by activating the ERK pathway. J Cancer 12:5473-5485 (2021). PubMed: 34405010
- Zhu X et al. ANGPTL4 Attenuates Ang II-Induced Atrial Fibrillation and Fibrosis in Mice via PPAR Pathway. Cardiol Res Pract 2021:9935310 (2021). PubMed: 34422410
- Martinho JP et al. Impairment of the angiogenic process may contribute to lower success rate of root canal treatments in diabetes mellitus. Int Endod J N/A:N/A (2021). PubMed: 33999433