概述

  • 产品名称
    Anti-Angiopoietin 1 + Angiopoietin 2抗体
  • 描述
    兔多克隆抗体to Angiopoietin 1 + Angiopoietin 2
  • 宿主
    Rabbit
  • 经测试应用
    适用于: ICC/IF, IHC-Fr, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human Angiopoietin 1 + Angiopoietin 2 aa 482-496 (C terminal).
    Sequence:

    YSLKATTMMIRPADF


    (Peptide available as ab98299)

  • 阳性对照
    • Human placenta tissue, rat brain lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservatives: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
    Constituents: 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate, 2.5% BSA
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab65835 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
IHC-Fr Use at an assay dependent concentration. PubMed: 20562294
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 57 kDa).
IHC-P Use at an assay dependent concentration. PubMed: 21439064

靶标

图片

  • This image shows human placental tissue stained with ab65835 at 2µg/ml.
  • Anti-Angiopoietin 1 + Angiopoietin 2 antibody (ab65835) at 2 µg/ml + rat brain tissue lysate

    Predicted band size: 57 kDa
    Observed band size: 65 kDa
    why is the actual band size different from the predicted?

  • ICC/IF image of ab65835 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65835, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

文献

This product has been referenced in:
  • Lun W  et al. MiR-218 regulates epithelial-mesenchymal transition and angiogenesis in colorectal cancer via targeting CTGF. Cancer Cell Int 18:83 (2018). Read more (PubMed: 29977158) »
  • Prapansilp P  et al. A clinicopathological correlation of the expression of the angiopoietin-Tie-2 receptor pathway in the brain of adults with Plasmodium falciparum malaria. Malar J 12:50 (2013). IHC-P ; Human . Read more (PubMed: 23383853) »
See all 14 Publications for this product

客户评价及客户问答

1-10 of 14 Abreviews or Q&A

Answer

Thank you for contacting us.

The online datasheet is the best source for information about this antibody, ab65385:

Click here (or use the following: https://www.abcam.com/index.html?datasheet=65385).

There are six references currently listed but I have not checked them to determine if they are using the antibody to identify newly formed blood vessels. If you have specific questions that are not answered by the datasheet, please contact us.

Have you considered other markers of angiogenesis, such as CD105, or VEGFR1? We recommend antibody ab11414 and antibody ab32152, and we have many others:

Click here (or use the following: https://www.abcam.com/index.html?datasheet=11414).

Click here (or use the following: https://www.abcam.com/index.html?datasheet=32152).

Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab56301. The item will be shipped on Monday for Tuesday delivery.
To check the status of the order please contact our Customer Service team and reference this number.
Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
I wish you the best of luck with your research.

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Answer

Thank you for your reply.
I have aligned the human angiopoietin 1 and 2 proteins and the overall sequence homology is only 61%.
I would recommend ab64015, since the immunogen does not align well with Ang1 (63% homology)
https://www.abcam.com/Angiopoietin-2-antibody-ab64015.html
or ab56301 (https://www.abcam.com/Angiopoietin-2-antibody-MM0020-1F29-ab56301.html) as this antibody recognizes Angiopoietin 2 in western blot without cross reactivity with Angiopoietin 1.
Please let me know which of the 2 antibodies you would prefer and I will arrange the shipment for you. The order I have is the correct one. Thank you for the additional order information.
Also, if the shipping address has changed in the meantime (since August), please let me know the new address.
I look forward to hear back from you.

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Answer

Thank you for contacting us.
I am very sorry to hear that you had not been informed about the recall for ab65835. I believe it was sent to the person placing the order with us.
I would be more than happy to send you a replacement antibody for Ang2.
You mentioned that you are using human cells. Could you please also let me know which application you will use the antibody for so that I can select the correct one?
We have currently 5 antibodies: ab8452, ab125692, ab64015, ab56301 and ab10601.


I look forward to hear back from you and resolve this case for you.

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Question

Catalog number(s): 65835

Product(s): Rabbit polyclonal to ANGPT1 + ANGPT2

Survey results
----------------------------------------------------------------------------------
Telephone section
Q. Did you contact Abcam's Scientific Support by telephone in reference to this complaint?
A. Yes
Q. Was it clear from the phone options which number you needed to press to get your issue resolved?
A. I do not remember

Protocol advice section
Q. Did Abcam's Scientific Support give you protocol advice?
A.Yes
Q. How did you feel about being given protocol advice?
A.I was not expecting to be given protocol advice, but was happy to receive this
Q. Did the product work successfully after following the advice?
A.No
Q. Why have you not tried the protocol advice? (Select all that apply.)
A.

Free of charge replacement section
Q. Did Abcam's Scientific Support give you a free of charge replacement?
A. Yes

Q. Did the replacement product work successfully?
A.Yes
Credit note / refund section
Q. Did Abcam's Scientific Support give you a credit note or a refund?
A.No

Q. Would you have preferred a different outcome from receiving a credit note or refund?
A.
Overall satisfaction section
Q. How satisfied are you with how the complaint was handled?
A.Very satisfied
Q. Could we have done anything to better resolve the problem?
A.
Q. Would you mind if our scientific support team contacted you to better understand what happened and reach an agreeable solution?
A.No, I would not mind
Q. Please share with us any final thoughts you may have about Abcam, the complaints process, or our products.
A.

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Answer

Thank you for your participation in Abcam's phone survey.

If there is anything else I can do for you, or if you have questions to any of our products (e.g. primary antibodies, lysates, secondary antibodies, kits, controls, gels, proteins/peptides, slides, biochemicals, conjugation kits), please let me know.

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Question
Answer

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.
To check the status of the order please contact our Customer Service team and reference this number.
Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
I wish you the best of luck with your research.

Read More

Answer

Thank you for contacting Abcam.
For ab15429 we have found that there are problems with this antibody in western blot and that we can no longer guarantee that it will work in this application. However it is still guaranteed to work in IP and IHC-P. If you had originally purchased this antibody to work in WB and you are having problems with it, then I will be happy to replace the antibody.
As for ab65835 we have found that cross reacting with angiopoietin 1 and therefore we would be happy to either replace this antibody with another angiopoietin 1 antibody of your choice, possible ab8451 or ab94684.
I do apologize for any inconvenience these issues may have caused you and I look forward to hearing from you so that I can get this sorted out.
If there is anything I can help with, please let me know.

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Question

Thanks for following up on this issue. I have also performed an additional western blot testing the antibody on mouse tissue, and it shows clearly that the antibody recognizes both Angpt1 and Angp2--as the band intensity is reduced dramatically when tissue from either Angpt1 or Angpt2 knockout animals is used. I've attached this western blot, as well as a graph showing the relative band intensity when normalized to B-actin. The apparent cross-reactivity is unfortunate, as the antibody produces clean blots and seems to have a high specificity to the band of the appropriate size for Angpt2 / Angpt 1. I have mixed feelings about abcam's handling of this issue--When I first called and expressed my concern with the antibody, the rep I spoke with was very apologetic and offered a different Ang2 antibody as a replacement. At the time this seemed like solid customer service and I was fairly satisfied. However, now that I've read the email below it seems that abcam should have been aware that there was an issue with this product and by failure to note it on the product datasheet, has caused me to waste a significant amount of time verifying a known problem with the product. Had I known about the cross-reactivity before placing the order I could have simply ordered the second product (which I am now in the process of testing) and bypassed the issue entirely. That being said, I'm glad that you passed this information along as it gives me more confidence in the results of my testing. I have always had (and continue to hold) a high regard for the quality of abcam products and customer service, and I realize that not every antibody can be perfect but I wish that in this case more care had been taken to obtain the relevant specificity information from the supplier and relay that information to your customers. Postscript: As I was writing the above, we received a box of cookies etc. shipped from abcam. The thought is appreciated, and throughout I've been impressed by how you've stood behind the product (I wouldn't have bothered contacting "other company" about a bad antibody for example) it's just unfortunate that the information wasn't relayed at the outset.

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Answer

Thank you for your reply. I very much appreciate your honest feedback as well as for sharing you findings with the knockout data. I will definitely pass the WB data on to the supplying lab - for their consideration and re-evalution of the antibody. Also, I will make sure that the datasheet is updated accordingly as soon as possible. I completely understand your frustration about the lack of such crossreactivity information on the datasheet and will let the supplying lab know that as well, so that - going forward - more care is being taken in checking crossreactivity beforehand as well as passing such information on to us. Usually crossreactivity is being checked by sequence alignments as it would be too costly to use knockout animals for all the different target proteins. Therefore, the feedback you provided is very valuable for us. Again, please accept my sincere apologies for the problems you encountered and the time, reagents and samples spent. Please let me know if there is anything else I can do for you. Also, please let me know how the replacement antibody is working for you. Furthermore, if you need any additional advice or information, please don't hesitate to contact me.

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Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I will pass them on to the lab for further evaluation. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab8452. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

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Answer

Thank you for contacting us. Your credit note ID is XXXXXXX. I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways: (1) Redeemed against the original invoice if this hasn't already been paid. (2) Held on the account for use against a future order. (3) A full refund can be offered where no other invoices are outstanding. Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge. To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website. The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice  

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1-10 of 14 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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