重组Anti-Androgen Receptor (AR-V7 specific)抗体[EPR15656] - Low endotoxin,Azide free (ab209491)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15656] to Androgen Receptor (AR-V7 specific) - Low endotoxin, Azide free
- Suitable for: IHC-P, WB
- Reacts with: Human
概述
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产品名称
Anti-Androgen Receptor (AR-V7 specific)抗体[EPR15656] - Low endotoxin,Azide free
参阅全部 Androgen Receptor (AR-V7 specific) 一抗 -
描述
兔单克隆抗体[EPR15656] to Androgen Receptor (AR-V7 specific) - Low endotoxin,Azide free -
宿主
Rabbit -
特异性
This target is a splice variant of AR lacking the C-terminal androgen binding site.
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经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: 22Rv1 whole cell lysate. IHC-P: Human prostate cancer tissue.
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常规说明
ab209491 is the carrier-free version of ab198394.
For Androgen receptor variant 5,6,7es (ARv567es) specific RabMAb product - see ab200827.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR15656 -
同种型
IgG
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab209491于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 80 kDa (predicted molecular weight: 68 kDa).
Please check the parent abID, ab198394, for more information on dilutions. |
说明 |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Detects a band of approximately 80 kDa (predicted molecular weight: 68 kDa). Please check the parent abID, ab198394, for more information on dilutions. |
靶标
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相关性
Belongs to the nuclear hormone receptor family. Contains nuclear receptor DNA-binding domain. Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. This target is a splice variant of Androgen receptor (UniProt P10275) lacking the C-terminal androgen binding site. -
细胞定位
Nuclear -
数据库链接
- SwissProt: C0JKD3 Human
- SwissProt: E7EVX6 Human
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别名
- Androgen receptor splice variant 3 antibody
- AR-V7 antibody
- AR-V7(AR3) antibody
see all
图片
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Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue labeling Androgen Receptor(AR-V7) with ab198394 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Counterstained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Nucleus staining on cancer cells of Human prostate cancer (case#:F123132) is observed.
Cancer Res. 2014 Apr 15;74(8):2270-82. PMID:24556717
Cancer Res. 2009 Jan 1;69(1):16-22. PMID:19117982
PLoS One. 2011;6(11):e27970. PMID:22114732
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198394).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue labeling Androgen Receptor(AR-V7) with ab198394 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Counterstained with Hematoxylin.
Negative on cancer cells of Human prostate cancer (case#:F11194)
Cancer Res. 2014 Apr 15;74(8):2270-82. PMID:24556717
Cancer Res. 2009 Jan 1;69(1):16-22. PMID:19117982
PLoS One. 2011;6(11):e27970. PMID:22114732
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198394).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue labeling Androgen Receptor(AR-V7) with ab198394 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Counterstained with Hematoxylin.
Negative on cancer cells of Human prostate cancer (case#:F121524)
Cancer Res. 2014 Apr 15;74(8):2270-82. PMID:24556717
Cancer Res. 2009 Jan 1;69(1):16-22. PMID:19117982
PLoS One. 2011;6(11):e27970. PMID:22114732
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198394).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Androgen Receptor(AR-V7) with ab198394 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Counterstained with Hematoxylin.
Negative staining on normal Human liver tissue.
Cancer Res. 2014 Apr 15;74(8):2270-82. PMID:24556717
Cancer Res. 2009 Jan 1;69(1):16-22. PMID:19117982
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198394).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling AR-V7 with ab198394 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Counterstained with Hematoxylin.
Negative staining on Human hepatocellular carcinoma.
Cancer Res. 2014 Apr 15;74(8):2270-82. PMID:24556717
Cancer Res. 2009 Jan 1;69(1):16-22. PMID:19117982
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198394).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] - Low endotoxin, Azide free (ab209491) + 22RV1 (Human prostate carcinoma cell line) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Predicted band size: 68 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM/TBST
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (1)
ab209491 被引用在 1 文献中.
- Hille C et al. Detection of Androgen Receptor Variant 7 (ARV7) mRNA Levels in EpCAM-Enriched CTC Fractions for Monitoring Response to Androgen Targeting Therapies in Prostate Cancer. Cells 8:N/A (2019). PubMed: 31514447