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Signal Transduction Protein Phosphorylation Ser / Thr Kinases Other Kinases

Anti-AMPK alpha 2抗体(ab3760)

  • Datasheet
  • SDS
Reviews (6)Q&A (2)References (30)

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Western blot - Anti-AMPK alpha 2 antibody (ab3760)
  • Immunocytochemistry/ Immunofluorescence - Anti-AMPK alpha 2 antibody (ab3760)

Key features and details

  • Rabbit polyclonal to AMPK alpha 2
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-AMPK alpha 2抗体
    参阅全部 AMPK alpha 2 一抗
  • 描述

    兔多克隆抗体to AMPK alpha 2
  • 宿主

    Rabbit
  • 特异性

    Does not cross-react with AMPK alpha 1.
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Mouse
    Human
    See all applications and species data
  • 免疫原

    Synthetic peptide (Human) - which represented a portion of AMP activated protein kinase, alpha-2 catalytic subunit encoded within exon 7.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液

    pH: 7
    Preservative: 0.1% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Cardiovascular
    • Lipids / Lipoproteins
    • Fatty Acids
    • Metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Fatty acids
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Fatty acid oxidation

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human AMPK alpha 2 protein (ab159189)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab3760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
ICC/IF
Human
WB
Mouse
Human
All applications
Rabbit
Guinea pig
Pig
Chimpanzee
Rhesus monkey
Gorilla
Chinese hamster
Orangutan
Elephant
应用 Ab评论 说明
ICC/IF
Use a concentration of 1 µg/ml.
WB (5)
1/500 - 1/5000. Predicted molecular weight: 63 kDa.
说明
ICC/IF
Use a concentration of 1 µg/ml.
WB
1/500 - 1/5000. Predicted molecular weight: 63 kDa.

靶标

  • 功能

    Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3. Involved in insulin receptor/INSR internalization (PubMed:25687571). AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A. Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm. In response to stress, phosphorylates 'Ser-36' of histone H2B (H2BS36ph), leading to promote transcription. Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2, RPTOR and ATG1/ULK1: in response to nutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2. In response to nutrient limitation, promotes autophagy by phosphorylating and activating ATG1/ULK1. AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1, leading to destabilize it. May regulate the Wnt signaling pathway by phosphorylating CTNNB1, leading to stabilize it. Also phosphorylates CFTR, EEF2K, KLC1, NOS3 and SLC12A1. Plays an important role in the differential regulation of pro-autophagy (composed of PIK3C3, BECN1, PIK3R4 and UVRAG or ATG14) and non-autophagy (composed of PIK3C3, BECN1 and PIK3R4) complexes, in response to glucose starvation. Can inhibit the non-autophagy complex by phosphorylating PIK3C3 and can activate the pro-autophagy complex by phosphorylating BECN1.
  • 序列相似性

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
    Contains 1 protein kinase domain.
  • 结构域

    The AIS (autoinhibitory sequence) region shows some sequence similarity with the ubiquitin-associated domains and represses kinase activity.
  • 翻译后修饰

    Ubiquitinated.
    Phosphorylated at Thr-172 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Also phosphorylated at Thr-172 by CAMKK2; triggered by a rise in intracellular calcium ions, without detectable changes in the AMP/ATP ratio. CAMKK1 can also phosphorylate Thr-172, but at much lower level. Dephosphorylated by protein phosphatase 2A and 2C (PP2A and PP2C). Phosphorylated by ULK1; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK. Dephosphorylated by PPM1A and PPM1B at Thr-172 (mediated by STK11/LKB1).
  • 细胞定位

    Cytoplasm. Nucleus. In response to stress, recruited by p53/TP53 to specific promoters.
  • Target information above from: UniProt accession P54646 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 5563 Human
    • Entrez Gene: 108079 Mouse
    • Entrez Gene: 100172060 Orangutan
    • Entrez Gene: 397504 Pig
    • Omim: 600497 Human
    • SwissProt: P54646 Human
    • SwissProt: Q8BRK8 Mouse
    • SwissProt: Q5RD00 Orangutan
    • SwissProt: Q28948 Pig
    • Unigene: 437039 Human
    • Unigene: 48638 Mouse
    see all
  • 别名

    • 5'-AMP-activated protein kinase catalytic subunit alpha-2 antibody
    • AAPK2_HUMAN antibody
    • ACACA kinase antibody
    • Acetyl CoA carboxylase kinase antibody
    • Acetyl-CoA carboxylase kinase antibody
    • AMPK alpha 2 chain antibody
    • AMPK subunit alpha-2 antibody
    • AMPK2 antibody
    • AMPKa2 antibody
    • AMPKalpha2 antibody
    • HMGCR kinase antibody
    • Hydroxymethylglutaryl CoA reductase kinase antibody
    • Hydroxymethylglutaryl-CoA reductase kinase antibody
    • PRKAA antibody
    • PRKAA2 antibody
    • Protein kinase AMP activated alpha 2 catalytic subunit antibody
    • Protein kinase AMP activated catalytic subunit alpha 2 antibody
    see all

图片

  • Western blot - Anti-AMPK alpha 2 antibody (ab3760)
    Western blot - Anti-AMPK alpha 2 antibody (ab3760)
    All lanes : Anti-AMPK alpha 2 antibody (ab3760) at 0.4 µg/ml

    Lane 1 : HeLa (Human epithelial adenocarcinoma cell line) whole cell lysate
    Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : Renca (Mouse renal adenocarcinoma cell line) whole cell lysate
    Lane 5 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Predicted band size: 63 kDa


    Exposure time: 3 minutes


    Chemiluminescence detection.

  • Immunocytochemistry/ Immunofluorescence - Anti-AMPK alpha 2 antibody (ab3760)
    Immunocytochemistry/ Immunofluorescence - Anti-AMPK alpha 2 antibody (ab3760)

    ICC/IF image of ab3760 stained MCF7 (Human breast adenocarcinoma cell line) cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3760, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

实验方案

  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

    • Datasheet
    • SDS
  • 文献 (30)

    发表研究结果有使用 ab3760?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3760 被引用在 30 文献中.

    • Yang W  et al. Brain-specific suppression of AMPKa2 isoform impairs cognition and hippocampal LTP by PERK-mediated eIF2a phosphorylation. Mol Psychiatry N/A:N/A (2020). PubMed: 32366952
    • Zhang Q  et al. miR-4999-5p Predicts Colorectal Cancer Survival Outcome and Reprograms Glucose Metabolism by Targeting PRKAA2. Onco Targets Ther 13:1199-1210 (2020). PubMed: 32103988
    • Johnson J  et al. Targeting PI3K and AMPKa Signaling Alone or in Combination to Enhance Radiosensitivity of Triple Negative Breast Cancer. Cells 9:N/A (2020). PubMed: 32438621
    • Zhao J  et al. Down-regulation of AMPK signaling pathway rescues hearing loss in TFB1 transgenic mice and delays age-related hearing loss. Aging (Albany NY) 12:5590-5611 (2020). PubMed: 32240104
    • Wang BZ  et al. AMPK Signaling Regulates the Age-Related Decline of Hippocampal Neurogenesis. Aging Dis 10:1058-1074 (2019). PubMed: 31595203
    View all Publications for this product

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    1-5 of 5 Abreviews or Q&A

    Western blot abreview for Anti-AMPK alpha 2 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (squeletal muscle, adipose tissus)
    Gel Running Conditions
    Reduced Denaturing (gradient gel 4-15%)
    Loading amount
    25 µg
    Specification
    squeletal muscle, adipose tissus
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Feb 22 2017

    Western blot abreview for Anti-AMPK alpha 2 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (Schwann cells)
    Loading amount
    20 µg
    Specification
    Schwann cells
    Gel Running Conditions
    Reduced Denaturing (10% SDS gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jun 26 2012

    Western blot abreview for Anti-AMPK alpha 2 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Primary hepatocytes (liver))
    Loading amount
    24 µg
    Specification
    Primary hepatocytes (liver)
    Gel Running Conditions
    Reduced Non-Denaturing (Native) (4-20%)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Apr 02 2012

    Western blot abreview for Anti-AMPK alpha 2 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (Rat heart cell (cardiac myocyte) lysates prepared)
    Loading amount
    20 µg
    Specification
    Rat heart cell (cardiac myocyte) lysates prepared
    Treatment
    I/R plus treatment of urocortin (Ucn1) or I/R plus treatment with Ucn2
    Gel Running Conditions
    Reduced Denaturing (10% SDS PAGE)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Prof. Paul Townsend

    Verified customer

    提交于 Mar 24 2010

    Western blot abreview for Anti-AMPK alpha 2 antibody

    Excellent
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mouse skeletal muscle cell)
    Loading amount
    25 µg
    Specification
    mouse skeletal muscle cell
    Gel Running Conditions
    Reduced Denaturing (gel 10%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    提交于 Nov 24 2009

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