Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172)抗体(ab194920)
Key features and details
- Rabbit polyclonal to AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172)
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172)抗体 -
描述
兔多克隆抗体to AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse -
免疫原
Synthetic peptide within Human AMPK alpha 1 (phospho T183). The exact sequence is proprietary. This phospho site has 100% homology to AMPK alpha 2 (phospho T172)
Database link: Q13131 -
阳性对照
- WB: C2C12 cells treated by Oligomycin.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab194920于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/2000.
|
说明 |
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WB
1/500 - 1/2000. |
靶标
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细胞定位
AMPK alpha 2: Cytoplasm. Nucleus. In response to stress, recruited by p53/TP53 to specific promoters. -
数据库链接
- Entrez Gene: 105787 Mouse
- Entrez Gene: 108079 Mouse
- SwissProt: Q5EG47 Mouse
- SwissProt: Q8BRK8 Mouse
- Unigene: 207004 Mouse
- Unigene: 48638 Mouse
图片
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All lanes : Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody (ab194920) at 1/2000 dilution
Lane 1 : C2C12 cell lysate
Lane 2 : Treated C2C12 cell lysate, cells were treated by Oligomycin (0.5uM) for 30 minutes.
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L)
Developed using the ECL technique.
Exposure time: 1 secondBlocking buffer: 3% BSA.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab194920 被引用在 6 文献中.
- Huang CC et al. Metformin: a novel promising option for fertility preservation during cyclophosphamide-based chemotherapy. Mol Hum Reprod 27:N/A (2021). PubMed: 33543290
- Fan H et al. Combined intake of blueberry juice and probiotics ameliorate mitochondrial dysfunction by activating SIRT1 in alcoholic fatty liver disease. Nutr Metab (Lond) 18:50 (2021). PubMed: 33971886
- An Y et al. SIRT1 inhibits chemoresistance and cancer stemness of gastric cancer by initiating an AMPK/FOXO3 positive feedback loop. Cell Death Dis 11:115 (2020). PubMed: 32051395
- Li X et al. Puerarin Attenuates Diabetic Nephropathy by Promoting Autophagy in Podocytes. Front Physiol 11:73 (2020). PubMed: 32116781
- Kan J et al. S100A16, a novel lipogenesis promoting factor in livers of mice and hepatocytes in vitro. J Cell Physiol 234:21395-21406 (2019). PubMed: 31069793
- Li Y et al. AMP-activated protein kinase stimulates osteoblast differentiation and mineralization through autophagy induction. Int J Mol Med 41:2535-2544 (2018). PubMed: 29484369