AMPK alpha 1 overexpression 293T裂解物(whole cell) (ab94311)
概述
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产品名称
AMPK alpha 1 overexpression 293T裂解物(whole cell)
参阅全部 AMPK alpha 1 细胞裂解液 -
常规说明
ab94311 is a 293T cell transfected lysate in which Human AMPK alpha 1 has been transiently over-expressed using a pCMV-AMPK alpha 1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
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经测试应用
适用于: WBmore details
性能
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Mycoplasma free
Yes -
形式
Liquid -
存放说明
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
存储溶液
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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研究领域
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背景
Function: Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit. Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. Contains 1 protein kinase domain.
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab94311于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent dilution.
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说明 |
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WB
Use at an assay dependent dilution. |
图片
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ab94311 at 15μg/lane on an SDS-PAGE gel.
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All lanes : Anti-AMPK alpha 1 antibody (ab89214) at 1/500 dilution
Lane 1 : AMPK alpha 1 293T Transfected Lysate - Positive Control (ab94311)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugate at 1/2500 dilution
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab94311 尚未被引用在任何文献中。