Anti-Amphiregulin抗体(ab180722)
Key features and details
- Rabbit polyclonal to Amphiregulin
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
-
产品名称
Anti-Amphiregulin抗体
参阅全部 Amphiregulin 一抗 -
描述
兔多克隆抗体to Amphiregulin -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant full length protein corresponding to Human Amphiregulin aa 20-252. Mature Protein without signal peptide.
Database link: P15514 -
阳性对照
- WB: MCF7, A549, HT29 and Mouse brain lysates.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab180722于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (2) |
1/500 - 1/2000. Predicted molecular weight: 28 kDa.
|
说明 |
---|
WB
1/500 - 1/2000. Predicted molecular weight: 28 kDa. |
靶标
-
功能
Bifunctional growth-modulating glycoprotein. Inhibits growth of several human carcinoma cells in culture and stimulates proliferation of human fibroblasts and certain other tumor cells. -
序列相似性
Belongs to the amphiregulin family.
Contains 1 EGF-like domain. -
细胞定位
Membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 374 Human
- Entrez Gene: 727738 Human
- Entrez Gene: 11839 Mouse
- Omim: 104640 Human
- SwissProt: P15514 Human
- SwissProt: P31955 Mouse
- Unigene: 270833 Human
- Unigene: 645475 Human
see all -
别名
- A REG antibody
- Amphiregulin antibody
- Amphiregulin B antibody
see all
图片
-
Anti-Amphiregulin antibody (ab180722) at 1/1000 dilution + A549 (Human lung carcinoma cell line) whole cell lysate at 25 µg
Secondary
HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 28 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking buffer: 3% nonfat dry milk in TBST.
PMID: 9642297.
-
Anti-Amphiregulin antibody (ab180722) at 1/1000 dilution + Mouse brain lysate at 25 µg
Secondary
HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 28 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
Exposure time: 300 secondsBlocking buffer: 3% nonfat dry milk in TBST.
PMID: 9642297.
-
All lanes : Anti-Amphiregulin antibody (ab180722) at 1/500 dilution
Lane 1 : MCF7 cell extracts
Lane 2 : A549 cell extracts
Lane 3 : HT29 cell extracts
Predicted band size: 28 kDa
数据表及文件
-
SDS download
-
Datasheet download
文献 (3)
ab180722 被引用在 3 文献中.
- Geng H et al. HOXB13 suppresses proliferation, migration and invasion, and promotes apoptosis of gastric cancer cells through transcriptional activation of VGLL4 to inhibit the involvement of TEAD4 in the Hippo signaling pathway. Mol Med Rep 24:N/A (2021). PubMed: 34396425
- Chen S et al. Renal subcapsular delivery of PGE2 promotes kidney repair by activating endogenous Sox9+ stem cells. iScience 24:103243 (2021). PubMed: 34746706
- Dong X et al. Long Non-coding RNA MIR4435-2HG Promotes Colorectal Cancer Proliferation and Metastasis Through miR-206/YAP1 Axis. Front Oncol 10:160 (2020). PubMed: 32154166