Anti-AMBRA1抗体(ab223351)
Key features and details
- Rabbit polyclonal to AMBRA1
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-AMBRA1抗体
参阅全部 AMBRA1 一抗 -
描述
兔多克隆抗体to AMBRA1 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Rat, Human -
免疫原
Synthetic peptide within Human AMBRA1 aa 200-300 (internal sequence). The exact sequence is proprietary.
Database link: Q9C0C7 -
阳性对照
- WB: Rat brain lysate. ICC/IF: SK-N-BE cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab223351于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Predicted molecular weight: 143 kDa.
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ICC/IF |
1/100.
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说明 |
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WB
1/1000. Predicted molecular weight: 143 kDa. |
ICC/IF
1/100. |
靶标
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功能
Regulates autophagy and development of the nervous system. Involved in autophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation. -
序列相似性
Contains 3 WD repeats. -
细胞定位
Cytoplasmic vesicle > autophagosome. - Information by UniProt
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数据库链接
- Entrez Gene: 55626 Human
- Omim: 611359 Human
- SwissProt: Q9C0C7 Human
- Unigene: 654644 Human
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别名
- Activating molecule in beclin 1 regulated autophagy antibody
- Activating molecule in BECN1 regulated autophagy protein 1 antibody
- Activating molecule in BECN1-regulated autophagy protein 1 antibody
see all
图片
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4% formaldehyde fixed SK-N-BE cells stained for AMBRA1 (green) using ab223351 at 1/100 dilution in ICC/IF. Secondary: Goat Anti-Rabbit ATTO 488 at 1/200 for 60 minutes at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 minutes RT, 5 minutes RT.
(A) DAPI nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223351 (D) Merge.
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Anti-AMBRA1 antibody (ab223351) at 1/1000 dilution + Rat brain lysate at 15 µg
Secondary
Goat Anti-Rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 143 kDa
Exposure time: 6 minutesBlocking: 5% skimmed milk in 1X TBST.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab223351 尚未被引用在任何文献中。