AM404,Anandamide transport抑制剂(ab120095)
Key features and details
- Anandamide transport inhibitor
- CAS Number: 198022-70-7
- Soluble in DMSO to 50 mM and in ethanol to 50 mM
- Form / State: Solid
- Source: Synthetic
概述
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产品名称
AM404,Anandamide transport抑制剂 -
描述
Anandamide transport抑制剂 -
生物学描述
Competitive and selective anandamide transport inhibitor (IC50 = 1 μM). Active in vivo. Does not activate CB1 receptors or inhibit anandamide hydrolysis but has been shown to activate vanilloid receptors.
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CAS编号
198022-70-7 -
化学结构
性能
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化学名称
(5Z,8Z,11Z,14Z)-N-(4-Hydroxyphenyl)icosa-5,8,11,14-tetraenamide -
分子量
395.58 -
分子式
C26H37NO2 -
PubChem识别号
6604822 -
存放说明
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解度概述
Soluble in DMSO to 50 mM and in ethanol to 50 mM -
处理
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
Oc1ccc(NC(=O)CCC/C=CC/C=CC/C=CC/C=CCCCCC)cc1 -
来源
Synthetic
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研究领域
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipoprotein metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipases
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Fatty acids
图片
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2D chemical structure image of ab120095, AM404, Anandamide transport inhibitor
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SH-SY5Y cells were incubated at 37°C for 30 minutes with vehicle control (0 µM) and varied concentrations of AM404 (ab120095). Increased expression of c-Fos in SH-SY5Y cells correlates with an increase in AM404 concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab7963 at 1 µg/ml and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (1)
ab120095 被引用在 1 文献中.
- Kim J & Alger BE Reduction in endocannabinoid tone is a homeostatic mechanism for specific inhibitory synapses. Nat Neurosci 13:592-600 (2010). PubMed: 20348918