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Tags & Cell Markers Subcellular Markers Cytoskeleton Microtubules

Anti-alpha Tubulin抗体[TU-01] (ab7750)

  • Datasheet
  • SDS
Reviews (8) Submit a question References (25)

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Immunocytochemistry - Anti-alpha Tubulin antibody [TU-01] (ab7750)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [TU-01] (ab7750)
  • Western blot - Anti-alpha Tubulin antibody [TU-01] (ab7750)
  • Flow Cytometry (Intracellular) - Anti-alpha Tubulin antibody [TU-01] (ab7750)
  • Immunocytochemistry - Anti-alpha Tubulin antibody [TU-01] (ab7750)
  • Immunocytochemistry - Anti-alpha Tubulin antibody [TU-01] (ab7750)
  • Immunocytochemistry - Anti-alpha Tubulin antibody [TU-01] (ab7750)

Key features and details

  • Mouse monoclonal [TU-01] to alpha Tubulin
  • Suitable for: Flow Cyt (Intra), ICC, IHC-P, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

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概述

  • 产品名称

    Anti-alpha Tubulin抗体[TU-01]
    参阅全部 alpha Tubulin 一抗
  • 描述

    小鼠单克隆抗体[TU-01] to alpha Tubulin
  • 宿主

    Mouse
  • 经测试应用

    适用于: Flow Cyt (Intra), ICC, IHC-P, WBmore details
  • 种属反应性

    与反应: Mouse, Human
    预测可用于: Turkey, Pig, Saccharomyces cerevisiae, Paramecium tetraurelia, Nicotiana benthamiana
  • 免疫原

    Full length native protein (purified) corresponding to Pig alpha Tubulin aa 37-154.

  • 表位

    aa 65-97 on N-terminal structural domain
  • 阳性对照

    • ICC: HeLa and NIH/3T3 cells. IHC-P: Human skin tissue. Flow Cyt (Intra): HEK293 cells. WB: Jurkat, HeLa, HEK293T, U87-MG all under reducing conditions.
  • 常规说明

    This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • 存储溶液

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度

    Proprietary Purification
  • 纯化说明

    Purified from TCS. Purified by precipitation and chromatography. Purity >95% by SDS-PAGE.
  • 克隆

    单克隆
  • 克隆编号

    TU-01
  • 同种型

    IgG1
  • 研究领域

    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Microtubules
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • Tubulin
    • Isotype/Loading Controls
    • Loading Controls
    • Alpha Tubulin
    • Neuroscience
    • Processes

相关产品

  • Alternative Versions

    • Biotin Anti-alpha Tubulin antibody [TU-01] (ab74696)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Related Products

    • alpha Tubulin peptide (ab195584)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7750于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
Use a concentration of 1 - 4 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC
Use at an assay dependent concentration.
IHC-P
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB (5)
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 50 kDa.

reducing conditions.

说明
Flow Cyt (Intra)
Use a concentration of 1 - 4 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC
Use at an assay dependent concentration.
IHC-P
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 50 kDa.

reducing conditions.

靶标

  • 功能

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • 序列相似性

    Belongs to the tubulin family.
  • 翻译后修饰

    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • 细胞定位

    Cytoplasm > cytoskeleton.
  • Target information above from: UniProt accession P68366 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 7277 Human
    • Entrez Gene: 22145 Mouse
    • Omim: 191110 Human
    • SwissProt: P68366 Human
    • SwissProt: P68368 Mouse
    • Unigene: 75318 Human
    • Unigene: 1155 Mouse
    • 别名

      • Alpha-tubulin 1 antibody
      • ALS22 antibody
      • B ALPHA 1 antibody
      • bA408E5.3 antibody
      • H2 ALPHA antibody
      • Hum a tub1 antibody
      • Hum a tub2 antibody
      • LIS3 antibody
      • MGC171407 antibody
      • MGC55332 antibody
      • TBA4A_HUMAN antibody
      • Testis-specific alpha-tubulin antibody
      • TUBA1 antibody
      • TUBA1A antibody
      • tuba1l antibody
      • Tuba4a antibody
      • Tubulin alpha 1 chain antibody
      • Tubulin alpha antibody
      • Tubulin alpha-1 chain antibody
      • tubulin alpha-1B chain antibody
      • Tubulin alpha-4A chain antibody
      • Tubulin H2-alpha antibody
      • Tubulin, alpha 1 (testis specific) antibody
      • tubulin, alpha 1, like antibody
      • Tubulin, alpha 4a antibody
      • Tubulin, alpha, testis-specific antibody
      • Tubulin, alpha-1 antibody
      see all

    图片

    • Immunocytochemistry - Anti-alpha Tubulin antibody [TU-01] (ab7750)
      Immunocytochemistry - Anti-alpha Tubulin antibody [TU-01] (ab7750)

      ICC/IF image of ab7750 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7750, 5µg/ml) overnight at +4°C. The secondary antibody (green) was anti-mouse DyLight® 488 (ab96879) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [TU-01] (ab7750)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [TU-01] (ab7750)

      Ab7750 staining human normal skin. Staining is localised to the cytoplasm.
      Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
      Sections were stained using an automated system at room temperature. Sections were rehydrated and antigen retrieved with a retrieval buffer EDTA pH 9.0 . Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with an amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

    • Western blot - Anti-alpha Tubulin antibody [TU-01] (ab7750)
      Western blot - Anti-alpha Tubulin antibody [TU-01] (ab7750)

      Western blotting analysis labeling alpha-tubulin using ab7750 on lysates of various cell lines under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of ab74696 followed by IRDye800-conjugated streptavidin. A specific band was detected for alpha-tubulin at approximately 54 kDa.

    • Flow Cytometry (Intracellular) - Anti-alpha Tubulin antibody [TU-01] (ab7750)
      Flow Cytometry (Intracellular) - Anti-alpha Tubulin antibody [TU-01] (ab7750)

      Overlay histogram showing HEK293 cells stained with ab7750 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7750, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was anti-mouse DyLight® 488 (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Triton used under the same conditions. 

       

       

    • Immunocytochemistry - Anti-alpha Tubulin antibody [TU-01] (ab7750)
      Immunocytochemistry - Anti-alpha Tubulin antibody [TU-01] (ab7750)
      Immunocytochemistry/ Immunofluorescence analysis of NIH 3T3 (mouse embryonal fibroblast) cells labelling alpha tubulin (green) with ab7750. Vimentin was stained red as counterstain. DAPI was used to stain the nuclei blue. 
    • Immunocytochemistry - Anti-alpha Tubulin antibody [TU-01] (ab7750)
      Immunocytochemistry - Anti-alpha Tubulin antibody [TU-01] (ab7750)

      Immunocytochemistry analysis of HeLa (human cervix carcinoma) cells labelling alpha tubulin (red) with ab7750. DAPI was used to stain the nuclei blue. 

    • Immunocytochemistry - Anti-alpha Tubulin antibody [TU-01] (ab7750)
      Immunocytochemistry - Anti-alpha Tubulin antibody [TU-01] (ab7750)

      Immunocytochemistry analysis of NIH 3T3 (mouse embryonal fibroblast) cells labelling alpha tubulin (green) with ab7750. DAPI was used to stain the nuclei blue. 

    实验方案

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (25)

    发表研究结果有使用 ab7750?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab7750 被引用在 25 文献中.

    • Katsumata Y  et al. Alzheimer Disease Pathology-Associated Polymorphism in a Complex Variable Number of Tandem Repeat Region Within the MUC6 Gene, Near the AP2A2 Gene. J Neuropathol Exp Neurol 79:3-21 (2020). PubMed: 31748784
    • Heras-Garvin A  et al. Signs of Chronic Hypoxia Suggest a Novel Pathophysiological Event in a-Synucleinopathies. Mov Disord 35:2333-2338 (2020). PubMed: 32881058
    • Sudevan S  et al. Mitochondrial dysfunction causes Ca2+ overload and ECM degradation-mediated muscle damage in C. elegans. FASEB J 33:9540-9550 (2019). PubMed: 31162948
    • Wei J  et al. Anti-proliferative effect of isorhamnetin on HeLa cells through inducing G2/M cell cycle arrest. Exp Ther Med 15:3917-3923 (2018). PubMed: 29563987
    • Refolo V  et al. Progressive striatonigral degeneration in a transgenic mouse model of multiple system atrophy: translational implications for interventional therapies. Acta Neuropathol Commun 6:2 (2018). PubMed: 29298733
    View all Publications for this product

    客户评价及客户问答

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    提交评价 提交问题

    1-8 of 8 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-alpha Tubulin antibody [TU-01]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (oral epithelial)
    Specification
    oral epithelial
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. Tina Yuan

    Verified customer

    提交于 Sep 26 2018

    Western blot abreview for Anti-alpha Tubulin antibody [TU-01]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Plants Tissue lysate - whole (Nicotiana benthamiana)
    Gel Running Conditions
    Non-reduced Denaturing (4-20% precut gels)
    Loading amount
    10 µg
    Treatment
    lysis buffer 12h
    Specification
    Nicotiana benthamiana
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7.5% · Temperature: 20°C
    Read More

    Huan Zhang

    Verified customer

    提交于 Nov 13 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha Tubulin [TU-01] antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
    Sample
    Plants Cell (meiocyte)
    Specification
    meiocyte
    Permeabilization
    Yes - Triton X-100
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Jun 23 2014

    Western blot abreview for Anti-alpha Tubulin [TU-01] antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - other (U2OS)
    Specification
    U2OS
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    提交于 Mar 17 2014

    Immunoprecipitation abreview for Anti-alpha Tubulin antibody [TU-01]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (HEK 293T)
    Total protein in input
    350 µg
    Specification
    HEK 293T
    Immuno-precipitation step
    Protein A/G
    Read More

    Abcam user community

    Verified customer

    提交于 Jan 26 2012

    Western blot abreview for Anti-alpha Tubulin antibody [TU-01]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Hek 293)
    Loading amount
    10 µg
    Specification
    Hek 293
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 22°C
    Read More

    Dr. Chris Tracy

    Verified customer

    提交于 Jan 12 2012

    Western blot abreview for Anti-alpha Tubulin antibody [TU-01]

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (forebrain)
    Loading amount
    10 µg
    Specification
    forebrain
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jun 19 2007

    Western blot abreview for Anti-alpha Tubulin antibody [TU-01]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Dog Tissue lysate - whole (Heart)
    Loading amount
    80 µg
    Specification
    Heart
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More

    Dr. sudhish mishra

    Verified customer

    提交于 Apr 27 2007

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