Application
Western blot
Sample
Caenorhabditis elegans Cell lysate - whole cell (Whole cell lysate rom N2 worms)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Whole cell lysate rom N2 worms
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/5000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: anti-mouse LICOR 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Detection
Detection method
IF
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 50 kDa
Additional data
Additional Notes
Note: protein loaded in lanes 2-10 are whole cell lysates from N2 worms
For the uploaded image
Lane 1 - molecular weight ladder (PageRuler Prestained Protein Ladder)
Lane 2 - 5 ug
Lane 3 - 10 ug
Lane 4 - 12.5 ug
Lane 5 - 15 ug
Lane 6 - 20 ug
Lane 7 - 25 ug
Lane 8 - 30 ug
Lane 9 - 35 ug
Lane 10 - 40 ug
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Hannah Navarrete
Verified customer
提交于 Sep 06 2021