Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Gel Running Conditions
Reduced Denaturing (4-20% SDS-PAGE)
Loading amount
50 µg
Specification
HEK293
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C
Dilution
1/4000
Secondary antibody
Name
Non-Abcam antibody was used: goat-anti mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL prime
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 50 kDa
Positive control
HEK293
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提交于 Jun 24 2020