Application
Western blot
Sample
Human Tissue lysate - whole (skin tissue)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
30 µg
Treatment
UV B (100mJ/cm2)
Specification
skin tissue
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA in TBS
Dilution
1/1000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: anti-mouse IgG
Host species: Human
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Human
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
UV B treated (100mJ/cm2)
Exposure
3 second(s)
Bands
Specific: 52 kDa Non-specific: 52 kDa
Positive control
UV B non treated
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Apr 05 2019