Application
Western blot
Sample
Mouse Tissue lysate - whole (Mice Brain Regions)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
40 µg
Specification
Mice Brain Regions
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in tris buffered saline (TBST)
Dilution
1/750
Secondary antibody
Dilution
1/20000
Name
Non-Abcam antibody was used: LCR-926-68073 IRDye 680RD Donkey anti-Rabbit IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 680RD
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 680RD
Detection
Detection method
fluorescence
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 17kDa kDa Non-specific: 30, 75 kDa
Positive control
did not use a positive control, but we know that we can detect a band using antibodies from other companies in these specific brain regions of mice
Additional data
Additional Notes
Abtrial REFERENCE: 210118-000221-B (for ab138501)
The antibody does not appear to work well in mouse brain. We have used RIPA buffer + complete tablet for homogenisation and we used a supported nitrocellulose membrane.
lanes
1,2 olfactory bulb
3,4,5 striatum
6,7,8 substantia nigra
Please note that there is no exposure time for fluorescence signal detection. The imager just scans our blots to generate an image. I have included 5 min exposure to reflect the time of scanning.
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Aug 16 2021