Application
Western blot
Sample
Human Cell lysate - whole cell (SH-SY5Y cells)
Gel Running Conditions
Reduced Denaturing (8-12% gradient gel)
Loading amount
10 µg
Specification
SH-SY5Y cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in 0.2% Tween20 in PBS
Dilution
1/1000
Secondary antibody
Dilution
1/6000
Name
Non-Abcam antibody was used: anti-rabbit IgG(H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 15 kDa Non-specific: 80, 90, 115 kDa
Positive control
SH-SY5Y whole cell lysates
Additional data
Additional Notes
0.45 um PDVF membrane. Membrane fixation in 4% PFA in PBS for 20 min after transfer
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提交于 May 08 2020