Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human transitional cell carcinoma of the kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH 8
Permeabilization
No
Specification
Human transitional cell carcinoma of the kidney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
10% formalin
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 5% normal goat serum
Dilution
1/150
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: goat anti-rabbit Alexa-568
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Additional data
Additional Notes
1. anti alpha smooth muscle actin (red) showing staining of human cancer stroma (x40). 2. anti alpha smooth muscle actin (red) showing staining of human cancer stroma. Co-stained with E-cadherin (green) and Hoechst 33342 (nuclei, cyan) (x40).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Cameron Johnstone
Verified customer
提交于 Oct 04 2021