Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Monkey Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization
No
Specification
Liver
Blocking step
Milk + BSA + FBS as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 27°C
Fixative
Formaldehyde
Other product details
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/100
Secondary antibody
Dilution
1/750
Name
Non-Abcam antibody was used: Thermo Life
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor 488
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor 488
Additional data
Additional Notes
Specimen: Monkey liver
Fixative: Formalin in Millonig buffer (48 hours)
Antigen retrieval in Pascal(R) chamber
Counterstaining: DAPI (Blue)
Image acquisition: Zeiss LSM 710 confocal microscope
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Marcelo Pelajo Machado
Verified customer
提交于 May 25 2017