Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Distal colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM Citrate buffer + 2 mM EDTA
Permeabilization
Yes - 0,05% Tween
Specification
Distal colon
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 20°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: Donkey serum 4% in PBS
Dilution
1/500
Secondary antibody
Dilution
1/400
Name
Non-Abcam antibody was used: Alexa Fluor® 488 AffiniPure Donkey Anti-Mouse IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Picture legend : DAPI (Blue), GFAP (Red), a-SMA (Green)
Abcam response
Thank you for participating in our AbTrial program and your valuable feedback. We are pleased that this antibody worked well for you in Mouse, a species that has not been tested before. We welcome any further feedback and AbReviews from other researchers who have used this antibody in Mouse which will help us assess how well the antibody works in this species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jan 25 2022