Application
Western blot
Sample
Human Cell lysate - whole cell (human primary dermal fibroblasts)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
25 µg
Specification
human primary dermal fibroblasts
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS, 0.05% Tween
Dilution
1/500
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: goat anti mouse HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 42 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
HERR DR. Vladimir Milenkovic
Verified customer
提交于 Nov 29 2018