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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin
使用敲除细胞株进行验证

Anti-alpha smooth muscle Actin抗体[1A4] (ab7817)

  • Datasheet
  • SDS
Reviews (102)Q&A (15)References (784)

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Immunocytochemistry - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)
  • Western blot - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)
  • Western blot - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)
  • Immunocytochemistry - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)
  • Immunocytochemistry - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)
  • Flow Cytometry - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)
  • Immunocytochemistry - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)
  • Immunocytochemistry - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)
  • Immunocytochemistry - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)
  • Immunocytochemistry - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)

Key features and details

  • Mouse monoclonal [1A4] to alpha smooth muscle Actin
  • Suitable for: ICC, IHC-P, WB, Flow Cyt
  • Knockout validated
  • Reacts with: Rat, Human
  • Isotype: IgG2a

Conjugates logo Related conjugates and formulations

FITC

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概述

  • 产品名称

    Anti-alpha smooth muscle Actin抗体[1A4]
    参阅全部 alpha smooth muscle Actin 一抗
  • 描述

    小鼠单克隆抗体[1A4] to alpha smooth muscle Actin
  • 宿主

    Mouse
  • 经测试应用

    适用于: ICC, IHC-P, WB, Flow Cytmore details
  • 种属反应性

    与反应: Rat, Human
    预测可用于: Mouse, Sheep, Rabbit, Cow, Pig, Mammals, Baboon
  • 免疫原

    Synthetic peptide corresponding to Human alpha smooth muscle Actin (N terminal).
    Database link: P62736

  • 阳性对照

    • WB: Human foreskin fibroblast lysate, human colon tissue lysate; NIH/3T3, SV40LT-SMC whole cell lysates. Flow Cyt: SV40LT-SMC. IHC-P: Human breast ductal carcinoma tissue. ICC: SV40LT-SMC cells.
  • 常规说明

    This antibody clone [1A4] is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • 纯度

    Protein G purified
  • 克隆

    单克隆
  • 克隆编号

    1A4
  • 同种型

    IgG2a
  • 轻链类型

    kappa
  • 研究领域

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin
    • Stem Cells
    • Lineage Markers
    • Mesoderm
    • Stem Cells
    • Mesenchymal Stem Cells
    • Myogenesis
    • Cardiovascular
    • Heart
    • Contractility
    • Contractile Proteins
    • Actins
    • Cardiovascular
    • Vasculature
    • Vasculature Markers
    • Arterial
    • Cardiovascular
    • Vasculature
    • Smooth muscle cell (SMC)
    • Cytoskeleton
    • Developmental Biology
    • Lineage specification
    • Mesoderm
    • Cardiovascular
    • Cardiovascular Markers
    • Cell Markers
    • Smooth Muscle Cells
    • Signal Transduction
    • Antibodies
    • alpha smooth muscle Actin

相关产品

  • Alternative Versions

    • Anti-pan Actin Antibody [4A4] (ab119952)
    • Biotin Anti-alpha smooth muscle Actin antibody [1A4] (ab125057)
    • Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (ab184675)
    • Alexa Fluor® 594 Anti-alpha smooth muscle Actin antibody [1A4] (ab202368)
    • HRP Anti-alpha smooth muscle Actin antibody [1A4] (ab203696)
    • Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free (ab240654)
    • FITC Anti-alpha smooth muscle Actin antibody [1A4] (ab8211)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
  • KO cell lines

    • Human ACTA2 knockout HeLa cell line (ab264014)
  • KO cell lysates

    • Human ACTA2 knockout HeLa cell lysate (ab264499)
  • Positive Controls

    • Jurkat whole cell lysate (ab7899)
    • A-431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human alpha smooth muscle Actin protein (ab134555)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
    • Phalloidin-iFluor 488 Reagent (ab176753)
    • Phalloidin-iFluor 647 Reagent (ab176759)
    • Anti-beta Tubulin antibody - Loading Control (ab6046)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7817于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC (3)
Use a concentration of 1 µg/ml.
IHC-P (39)
Use a concentration of 0.034 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB (15)
Use a concentration of 0.341 µg/ml.
Flow Cyt
Use a concentration of 1.137 µg/ml.
说明
ICC
Use a concentration of 1 µg/ml.
IHC-P
Use a concentration of 0.034 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use a concentration of 0.341 µg/ml.
Flow Cyt
Use a concentration of 1.137 µg/ml.

靶标

  • 功能

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • 疾病相关

    Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
  • 序列相似性

    Belongs to the actin family.
  • 细胞定位

    Cytoplasm > cytoskeleton.
  • Target information above from: UniProt accession P62736 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 101021287 Baboon
    • Entrez Gene: 515610 Cow
    • Entrez Gene: 59 Human
    • Entrez Gene: 11475 Mouse
    • Entrez Gene: 733615 Pig
    • Entrez Gene: 100009271 Rabbit
    • Entrez Gene: 81633 Rat
    • Entrez Gene: 101121051 Sheep
    • Omim: 102620 Human
    • SwissProt: P62739 Cow
    • SwissProt: P62736 Human
    • SwissProt: P62737 Mouse
    • SwissProt: P62740 Rabbit
    • SwissProt: P62738 Rat
    • Unigene: 500483 Human
    • Unigene: 213025 Mouse
    • Unigene: 195319 Rat
    • Unigene: 3114 Rat
    see all
  • 别名

    • a actin antibody
    • AAT6 antibody
    • ACTA_HUMAN antibody
    • ACTA2 antibody
    • Actin alpha 2 smooth muscle aorta antibody
    • Actin aortic smooth muscle antibody
    • Actin, aortic smooth muscle antibody
    • ACTSA antibody
    • ACTVS antibody
    • Alpha 2 actin antibody
    • Alpha actin 2 antibody
    • Alpha cardiac actin antibody
    • alpha sma antibody
    • Alpha-actin-2 antibody
    • Cell growth inhibiting gene 46 protein antibody
    • Cell growth-inhibiting gene 46 protein antibody
    • GIG46 antibody
    • Growth inhibiting gene 46 antibody
    • MYMY5 antibody
    see all

图片

  • Immunocytochemistry - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)
    Immunocytochemistry - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)
    ab7817 staining alpha smooth muscle Actin in SV40LT-SMC cells (positive control, top panel) and A431 cells (negative control, bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab7817 at 1μg/ml concentration and ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
    Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).
  • Western blot - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)
    Western blot - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)
    All lanes : Anti-alpha smooth muscle Actin antibody [1A4] (ab7817) at 1 µg/ml

    Lane 1 : NIH 3T3 whole cell lysate
    Lane 2 : SV40LT-SMC whole cell lysate
    Lane 3 : A431 whole cell lysate
    Lane 4 : A549 whole cell lysate
    Lane 5 : Jurkat whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Mouse IgG H&L (IRDye® 800RD) at 1/10000 dilution

    Observed band size: 42 kDa why is the actual band size different from the predicted?



    Gel type: MOPS

    Blocking buffer: 3% milk

    Loading control: alpha tubulin (ab52866), secondary Goat anti-Rabbit IgG H&L (IRDye® 680CW) preadsorbed (1:10000 dilution)

  • Western blot - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)
    Western blot - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)
    All lanes : Anti-alpha smooth muscle Actin antibody [1A4] (ab7817) at 1 µg/ml

    Lane 1 : Human colon tissue lysate
    Lane 2 : Mouse colon tissue lysate
    Lane 3 : Human Foreskin Fibroblast Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Mouse IgG H&L (IRDye® 800RD) at 1/10000 dilution

    Observed band size: 42 kDa why is the actual band size different from the predicted?



    Gel type: MOPS

    Blocking buffer: 3% milk

    Loading control: alpha tubulin (ab52866), secondary Goat anti-Rabbit IgG H&L (IRDye® 680CW) preadsorbed (1:10000 dilution)

  • Immunocytochemistry - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)
    Immunocytochemistry - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)

    This data was developed using the same antibody clone in a different buffer formulation that is PBS and sodium azide free (ab240654)

    ab240654 staining alpha smooth muscle Actin in SV40LT-SMC cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab240654 at 1µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)

    IHC image of alpha smooth muscle actin staining in a human breast ductal carcinoma formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab7817, 0.034µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Immunocytochemistry - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)
    Immunocytochemistry - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)
    ab7817 staining alpha smooth muscle Actin in wild-type HeLa cells (top panel) and ACTA2 knockout HeLa cells (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab7817 at 5μg/ml concentration and ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
    Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).
  • Flow Cytometry - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)
    Flow Cytometry - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)

    Overlay histogram showing SV40LT-SMC cells stained with ab7817 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab7817, 1.137µg/ml) for 30 min at 22°C. The secondary antibody used was Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113) at 1/2000 dilution for 30 min at 22°C

    Isotype control antibody (black line) was mouse IgG2a [18C8BC7AD10] (ab170191) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

    This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)This image is courtesy of an anonymous Abreview

    Ab7817 staining alpha smooth muscle actin in Mouse intestine tissue by Immunohistochemistry-Immunofluorescence. Tissue was fixed with formaldehyde and blocked with 100% Cas-block for 30 minutes at room temperature; antigen retrieval was performed by heat mediated citrate buffer, pH6. The sample was incubated with primary antibody at 0.034µg/ml for 16 hours at 4°C. An Alexa Fluor® 488 Goat anti-mouse IgG was used as the secondary antibody at 1/400 dilution. Autofluorescence was blocked with 0.1% Sudan Black in 70% ethanol for 10 minutes at room temperature after antigen retrieval, and followed with 3X wash with PBS-T after antigen retrieval. Image was taken with confocal microscope.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)This image was courtesy of an anonymous AbReview

    Formalin-fixed, paraffin-embedded Pig kidney tissue stained for alpha smooth muscle Actin (Green) using ab7817 at 0.034µg/ml followed by a Donkey anti-Mouse IgG (H+L) Alexa Fluor® 647 secondary antibody at 1/300 dilution. 

    Antigen retrieval: Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0

    The alpha smooth muscle Actin antibody signal is pseudo colored Green. The nuclei are Blue using Hoechst stain. The background tissue is stained Red

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)This image is courtesy of an Abreview submitted by Rudolf Jung

    ab7817 staining alpha smooth muscle actin in Human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and permeabilized with wash buffer with tween; antigen retrieval was by heat mediation in Tris-EDTA buffer, pH 9.0. Samples were incubated with primary antibody (0.034µg/ml in blocking buffer) for 30 minutes at 20°C. A HRP-conjugated Goat anti-mouse IgG polyclonal (undiluted) was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)This image is courtesy of an anonymous Abreview

    Immunohistochemical analysis of mouse aorta (A) or skin (B) tissue, staining alpha smooth muscle Actin with ab7817.

    Tissue was fixed with 10% Neutral Buffered Formalin and blocked with 1% serum for 45 minutes 21°C; antigen retrieval was by enzymatic method in 0.0001% Trypsin-CaCl. Samples were incubated with primary antibody (0.034µg/ml in 0.3% Triton X-100 in PBS) for 1 hour at 21&degC. A biotin-conjugated horse anti-mouse polyclonal IgG (1/50) was used as the secondary antibody.

    See Abreview

  • Immunocytochemistry - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)
    Immunocytochemistry - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)This image is courtesy of an Abreview submitted by Charles Pallangyo

    ab7817 staining alpha smooth muscle Actin (green) in Mouse primary colon myofibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with acetone and blocked with 5% BSA for 30 hours at 25°C. Samples were incubated with primary antibody (1/100 in PBS + 5% BSA) for 2 hours at 25°C. Donkey Anti-Mouse IgG H&L (DyLight® 488) (ab96875) (1/1000) was used as the secondary antibody. Costained with ab92547, Rabbit anti-Vimentin (red).

    See Abreview

  • Immunocytochemistry - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)
    Immunocytochemistry - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)Image from Davis FM et al., Nat Commun 25(7). Doi: 10.1038/ncomms13053. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Davis FM et al investigates the differentiation potential of mammary stem cells in adults. Ab7817 staining smooth muscle actin in EYFP+ cells from R26[CA]30EYFP mouse at 0.034µg/ml. 

  • Immunocytochemistry - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)
    Immunocytochemistry - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)This image is courtesy of an anonymous abreview.

    ab7817 staining alpha smooth muscle Actin in human IMR-90 (Human Lung Fibroblast Cell Line) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% TritonX-100 and blocked with 100% Cad-Block for 30 minutes at room temperature. Samples were incubated with primary antibody 3.41µg/ml in antibody diluent buffer for 16 hours at 4°C. An Alexa Fluor® 488-conjugated polyclonal Goat anti-mouse IgG, dilution 1/400, was used as secondary antibody.

    See Abreview

  • Immunocytochemistry - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)
    Immunocytochemistry - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)This image is courtesy of an Abreview submitted by Dr. Ho-Jae Lee

    ab7817 staining alpha smooth muscle Actin in mouse heart cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with TritonX-100 and blocked with 5% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody 6.82µg/ml in blocking buffer for 2 hours. An Alexa Fluor® 488-conjugated Donkey monoclonal to mouse IgG, dilution 1/200, was used as secondary antibody.

    See Abreview

实验方案

  • Mouse on Mouse staining protocol

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (784)

发表研究结果有使用 ab7817?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab7817 被引用在 784 文献中.

  • Ki SY  et al. Relationship between fatty infiltration and gene expression in patients with medium rotator cuff tear. J Shoulder Elbow Surg 30:387-395 (2021). PubMed: 32603896
  • Meng J  et al. Immune response drives outcomes in prostate cancer: implications for immunotherapy. Mol Oncol 15:1358-1375 (2021). PubMed: 33338321
  • Gao R  et al. Macrophage-derived netrin-1 drives adrenergic nerve-associated lung fibrosis. J Clin Invest 131:N/A (2021). PubMed: 33393489
  • Caetano AJ  et al. Defining human mesenchymal and epithelial heterogeneity in response to oral inflammatory disease. Elife 10:N/A (2021). PubMed: 33393902
  • Dong J  et al. Hepatocyte-specific IL11 cis-signaling drives lipotoxicity and underlies the transition from NAFLD to NASH. Nat Commun 12:66 (2021). PubMed: 33397952
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1-10 of 117 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-alpha smooth muscle Actin antibody [1A4]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Liver)
Permeabilization
No
Specification
Liver
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 21°C
Fixative
Acetone
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Abcam user community

Verified customer

提交于 Feb 09 2022

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-alpha smooth muscle Actin antibody [1A4]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Distal colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM Citrate buffer + 2 mM EDTA
Permeabilization
Yes - 0,05% Tween
Specification
Distal colon
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 20°C
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

提交于 Jan 25 2022

Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha smooth muscle Actin antibody [1A4]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Fibroblast)
Permeabilization
No
Specification
Fibroblast
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 21°C
Fixative
Acetone
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Abcam user community

Verified customer

提交于 Jan 17 2022

Immunocytochemistry abreview for Anti-alpha smooth muscle Actin antibody [1A4]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Human Cell (fibrobalst cells)
Specification
fibrobalst cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

提交于 Jan 04 2022

Immunohistochemistry (Frozen sections) abreview for Anti-alpha smooth muscle Actin antibody [1A4]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (rat brain)
Permeabilization
Yes - 0.5% TX100
Specification
rat brain
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Paraformaldehyde
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HERR DR. Vladimir Milenkovic

Verified customer

提交于 Dec 23 2021

Western blot abreview for Anti-alpha smooth muscle Actin antibody [1A4]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse primary astrocytes)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
25 µg
Specification
Mouse primary astrocytes
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
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HERR DR. Vladimir Milenkovic

Verified customer

提交于 Sep 24 2021

Immunohistochemistry (Frozen sections) abreview for Anti-alpha smooth muscle Actin antibody [1A4]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (breast)
Permeabilization
No
Specification
breast
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Read More

Abcam user community

Verified customer

提交于 Sep 20 2021

Immunohistochemistry (Frozen sections) abreview for Anti-alpha smooth muscle Actin antibody [1A4]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (lung)
Permeabilization
No
Specification
lung
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

提交于 Sep 20 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-alpha smooth muscle Actin antibody [1A4]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6
Permeabilization
No
Specification
skin
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

提交于 Jul 19 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-alpha smooth muscle Actin antibody [1A4]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
colon
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C
Fixative
10% NBF
Read More

Abcam user community

Verified customer

提交于 May 21 2021

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