Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Embryoid body Germ Layer)
Permeabilization
Yes - 0.05% Triton
Specification
Embryoid body Germ Layer
Blocking step
Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 20% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Dilution
1/100
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Life Tech
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 568
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 568
Additional data
Additional Notes
Embryoid bodies were fixed, cryoprotected 30% sucrose, and embedded in OCT. Samples were sectioned at 20um and stained for SMA 1:100
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Orla Fannon
Verified customer
提交于 Sep 12 2017