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Signal Transduction Protein Phosphorylation Tyrosine Kinases Other

Anti-Alpha B Crystallin (phospho S59)抗体(ab5577)

  • Datasheet
Reviews (3) Submit a question References (9)

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Western blot - Anti-Alpha B Crystallin (phospho S59) antibody (ab5577)

    Key features and details

    • Rabbit polyclonal to Alpha B Crystallin (phospho S59)
    • Suitable for: WB
    • Reacts with: Mouse, Rat, Cow, Human
    • Isotype: IgG

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    概述

    • 产品名称

      Anti-Alpha B Crystallin (phospho S59)抗体
      参阅全部 Alpha B Crystallin 一抗
    • 描述

      兔多克隆抗体to Alpha B Crystallin (phospho S59)
    • 宿主

      Rabbit
    • 经测试应用

      适用于: WBmore details
    • 种属反应性

      与反应: Mouse, Rat, Cow, Human
      预测可用于: Sheep, Rabbit, Hamster, Pig, Cynomolgus monkey
    • 免疫原

      Synthetic peptide corresponding to Human Alpha B Crystallin aa 54-64 (phospho S59).
      Sequence:

      FLRAPSWIDTG

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 常规说明

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • 存储溶液

      Preservative: 0.05% Sodium azide
      Constituents: PBS, 3% BSA
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Signal Transduction
      • Protein Phosphorylation
      • Tyrosine Kinases
      • Other
      • Neuroscience
      • Sensory System
      • Visual system
      • Neuroscience
      • Neurotransmission
      • Receptors / Channels
      • Tyrosine Kinase Receptors
      • Cancer
      • Cancer Metabolism
      • Cellular metabolic process
      • Metabolism
      • Types of disease
      • Cancer

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human Alpha B Crystallin protein (ab48779)

    应用

    Our Abpromise guarantee covers the use of ab5577 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 20 kDa.

    靶标

    • 功能

      May contribute to the transparency and refractive index of the lens.
    • 组织特异性

      Lens as well as other tissues.
    • 疾病相关

      Defects in CRYAB are the cause of myofibrillar alpha-B crystallin-related (MFM-CRYAB) [MIM:608810]. A neuromuscular disorder that results in weakness of the proximal and distal limb muscles, weakness of the neck, velopharynx and trunk muscles, hypetrophic cardiomyopathy, and cataract in a subset of patients.
    • 序列相似性

      Belongs to the small heat shock protein (HSP20) family.
    • 细胞定位

      Cytoplasm. Nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
    • Target information above from: UniProt accession P02511 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 281719 Cow
      • Entrez Gene: 102128339 Cynomolgus monkey
      • Entrez Gene: 1410 Human
      • Entrez Gene: 12955 Mouse
      • Entrez Gene: 100127443 Pig
      • Entrez Gene: 100009295 Rabbit
      • Entrez Gene: 25420 Rat
      • Entrez Gene: 497274 Sheep
      • Omim: 123590 Human
      • SwissProt: P02510 Cow
      • SwissProt: Q60HG8 Cynomolgus monkey
      • SwissProt: P02511 Human
      • SwissProt: P23927 Mouse
      • SwissProt: Q7M2W6 Pig
      • SwissProt: P41316 Rabbit
      • SwissProt: P23928 Rat
      • SwissProt: Q5ENY9 Sheep
      • Unigene: 53454 Human
      • Unigene: 178 Mouse
      • Unigene: 98208 Rat
      see all
    • 别名

      • AACRYA antibody
      • Alpha B crystallin antibody
      • Alpha crystallin B chain antibody
      • Alpha(B)-crystallin antibody
      • Alpha-crystallin B chain antibody
      • CRYA2 antibody
      • Cryab antibody
      • CRYAB_HUMAN antibody
      • Crystallin alpha B antibody
      • Crystallin alpha polypeptide 2 antibody
      • CTPP2 antibody
      • Heat shock 20 kD like protein antibody
      • Heat shock protein beta 5 antibody
      • Heat shock protein beta-5 antibody
      • HspB5 antibody
      • Renal carcinoma antigen NY REN 27 antibody
      • Renal carcinoma antigen NY-REN-27 antibody
      • Rosenthal fiber component antibody
      see all

    图片

    • Western blot - Anti-Alpha B Crystallin (phospho S59) antibody (ab5577)
      Western blot - Anti-Alpha B Crystallin (phospho S59) antibody (ab5577)

      ab5577 at 0.5µg/ml concentration staining approximately 20 KDa Crystallin Alpha B in lane 1) phospho-alpha-B crystallin (Ser59) and lane 2) non phosphorylated alpha-B crystallin in U-251 MG (formally U-373 MG) (Human brain glioma cell line) whole cell lysate.

    实验方案

    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet
  • 文献 (9)

    发表研究结果有使用 ab5577?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab5577 被引用在 9 文献中.

    • Dimauro I  et al. The early response of aB-crystallin to a single bout of aerobic exercise in mouse skeletal muscles depends upon fiber oxidative features. Redox Biol 24:101183 (2019). PubMed: 30974319
    • Yu W  et al. Lens-specific conditional knockout of Msx2 in mice leads to ocular anterior segment dysgenesis via activation of a calcium signaling pathway. Lab Invest N/A:N/A (2019). PubMed: 30683901
    • Zhang Y  et al. BYD Ameliorates Oxidative Stress-Induced Myocardial Apoptosis in Heart Failure Post-Acute Myocardial Infarction via the P38 MAPK-CRYAB Signaling Pathway. Front Physiol 9:505 (2018). PubMed: 29867551
    • Guo H  et al. Exercise-responsive phosphoproteins in the heart. J Mol Cell Cardiol 111:61-68 (2017). PubMed: 28826663
    • Kuipers HF  et al. Phosphorylation of aB-crystallin supports reactive astrogliosis in demyelination. Proc Natl Acad Sci U S A 114:E1745-E1754 (2017). PubMed: 28196893
    • Dammer EB  et al. Quantitative phosphoproteomics of Alzheimer's disease reveals cross-talk between kinases and small heat shock proteins. Proteomics 15:508-19 (2015). WB ; Human . PubMed: 25332170
    • Mitra A  et al. Cardioprotective role of P38 MAPK during myocardial infarction via parallel activation of a-crystallin B and Nrf2. J Cell Physiol 229:1272-82 (2014). PubMed: 24464634
    • Rajasekaran NS  et al. Sustained activation of nuclear erythroid 2-related factor 2/antioxidant response element signaling promotes reductive stress in the human mutant protein aggregation cardiomyopathy in mice. Antioxid Redox Signal 14:957-71 (2011). WB, IHC-Fr ; Mouse . PubMed: 21126175
    • Burniston JG  et al. Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity. Proteomics 11:3369-79 (2011). PubMed: 21751351

    客户评价及客户问答

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    1-3 of 3 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-alpha B Crystallin (phospho S59) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (adult kidney)
    Specification
    adult kidney
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid pH6
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
    Read More

    Mr. Carl Hobbs

    Verified customer

    提交于 Oct 21 2008

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-alpha B Crystallin (phospho S59) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (kidney and ovarian carcinoma)
    Specification
    kidney and ovarian carcinoma
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citric acid 10mM pH6
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
    Read More

    Mr. Carl Hobbs

    Verified customer

    提交于 Apr 22 2008

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-alpha B Crystallin (phospho S59) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (kidney)
    Specification
    kidney
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid 10mM pH6
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
    Read More

    Mr. Carl Hobbs

    Verified customer

    提交于 Apr 22 2008

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