Anti-Alpha B Crystallin抗体(ab13497)
Key features and details
- Rabbit polyclonal to Alpha B Crystallin
- Suitable for: IP, WB, ICC, IHC-Fr, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Chicken, Cow, Human, Pig
- Isotype: IgG
概述
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产品名称
Anti-Alpha B Crystallin抗体
参阅全部 Alpha B Crystallin 一抗 -
描述
兔多克隆抗体to Alpha B Crystallin -
宿主
Rabbit -
特异性
Does not cross-react with αA-crystallin.
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经测试应用
适用于: IP, WB, ICC, IHC-Fr, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Chicken, Cow, Human, Pig
预测可用于: Rabbit, Hamster -
免疫原
Synthetic peptide:
REEKPAVTAAPKK
conjugated to KLH, corresponding to amino acids 163-175 of Human alpha B Crystallin. -
阳性对照
- ICC: HeLa. (Heat shocked). WB: A431, HCT116, HeLa, HepG2, HEK293, HUVEC, Jurkat, MCF7, PC3 , and Rat brain cell lysate.
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常规说明
For maximum product recovery, after thawing, centrifuge the product vial before removing capThe Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituent: Whole serum -
Concentration information loading...
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纯度
Affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab13497于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
Use at an assay dependent concentration.
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WB | (3) |
1/1000 - 1/2000. Detects a band of approximately 22 kDa (predicted molecular weight: 20 kDa).
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ICC |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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IHC-P | (1) |
Use at an assay dependent concentration.
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ICC/IF |
1/120.
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说明 |
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IP
Use at an assay dependent concentration. |
WB
1/1000 - 1/2000. Detects a band of approximately 22 kDa (predicted molecular weight: 20 kDa). |
ICC
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
ICC/IF
1/120. |
靶标
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功能
May contribute to the transparency and refractive index of the lens. -
组织特异性
Lens as well as other tissues. -
疾病相关
Defects in CRYAB are the cause of myofibrillar alpha-B crystallin-related (MFM-CRYAB) [MIM:608810]. A neuromuscular disorder that results in weakness of the proximal and distal limb muscles, weakness of the neck, velopharynx and trunk muscles, hypetrophic cardiomyopathy, and cataract in a subset of patients. -
序列相似性
Belongs to the small heat shock protein (HSP20) family. -
细胞定位
Cytoplasm. Nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles. - Information by UniProt
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数据库链接
- Entrez Gene: 396089 Chicken
- Entrez Gene: 281719 Cow
- Entrez Gene: 1410 Human
- Entrez Gene: 12955 Mouse
- Entrez Gene: 100127443 Pig
- Entrez Gene: 100009295 Rabbit
- Entrez Gene: 25420 Rat
- Omim: 123590 Human
see all -
别名
- AACRYA antibody
- Alpha B crystallin antibody
- Alpha crystallin B chain antibody
see all
图片
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Anti-Alpha B Crystallin antibody (ab13497) at 1/1000 dilution + Human spinal cord tissue lysate - total protein (ab29188) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 20 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted? -
Western blot analysis of Human A431, HCT116, HeLa, HepG2, HEK293, HUVEC, Jurkat, MCF7, PC3 and T98G cell lysates showing detection of ~22 kDa Alpha B Crystallin protein using Rabbit Anti-Alpha B Crystallin Polyclonal Antibody (ab13497).
Lane 1: Molecular Weight Ladder (MW).
Lane 2: A431 cell lysates.
Lane 3: HCT116 cell lysates.
Lane 4: HeLa cell lysates.
Lane 5: HepG2 cell lysates.
Lane 6: HEK293 cell lysates.
Lane 7: HUVEC cell lysates.
Lane 8: Jurkat cell lysates.
Lane 9: MCF7 cell lysates.
Lane 10: PC3 cell lysates.
Lane 11: T98G cell lysates.
Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Alpha B Crystallin Polyclonal Antibody (ab13497) at 1/1000 dilution for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1/1000 dilution for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~22 kDa.
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Western blot analysis of Rat Brain cell lysates showing detection of ~22 kDa Alpha B Crystallin protein using Rabbit Anti-Alpha B Crystallin Polyclonal Antibody (ab13497).
Lane 1: Molecular Weight Ladder (MW).
Lane 2: Rat Brain cell lysates.
Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Alpha B Crystallin Polyclonal Antibody (ab13497) at 1/1000 dilution for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1/1000 dilution for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~22 kDa.
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Immunofluorescent analysis of 2% paraformaldehyde fixed Heat Shocked (at 42°C for 1h) Cervical cancer cell line (HeLa) for 20min at RT, labelling Alpha B Crystallin with ab13497 at 1/120 dilution for 12 hours at 4°C, followed by secondary antibody Goat Anti-Rabbit (FITC) (Green) at 1/200 dilution for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1/40000 dilution for 2 hours at RT. Image showing actin filament bundles, nuclear splicing speckles, and Exosome staining. (A) DAPI (blue) nuclear stain. (B) Anti-Alpha B Crystallin Antibody (green). (C) Composite image.
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Immunofluorescent analysis of 2% paraformaldehyde fixed Heat Shocked (at 42°C for 1h) Cervical cancer cell line (HeLa) for 20min at RT, labelling Alpha B Crystallin with ab13497 at 1/120 dilution for 12 hours at 4°C, followed by secondary antibody Goat Anti-Rabbit (APC) (Red) at 1/200 dilution for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1/40000 dilution for 2 hours at RT. Image showing actin filament bundles, nuclear splicing speckles, and Exosome staining. (A) DAPI (blue) nuclear stain. (B) Anti-Alpha B Crystallin Antibody (Red). (C) Composite image.
实验方案
数据表及文件
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Datasheet download
文献 (19)
ab13497 被引用在 19 文献中.
- Dumas SJ et al. Single-Cell RNA Sequencing Reveals Renal Endothelium Heterogeneity and Metabolic Adaptation to Water Deprivation. J Am Soc Nephrol 31:118-138 (2020). PubMed: 31818909
- Besnard V et al. Identification of periplakin as a major regulator of lung injury and repair in mice. JCI Insight 3:N/A (2018). PubMed: 29515024
- Zhang Y et al. BYD Ameliorates Oxidative Stress-Induced Myocardial Apoptosis in Heart Failure Post-Acute Myocardial Infarction via the P38 MAPK-CRYAB Signaling Pathway. Front Physiol 9:505 (2018). PubMed: 29867551
- Schmitt A et al. Effects of 10 weeks of regular running exercise with and without parallel PDTC treatment on expression of genes encoding sarcomere-associated proteins in murine skeletal muscle. Cell Stress Chaperones 23:1041-1054 (2018). PubMed: 29797237
- Guo H et al. Exercise-responsive phosphoproteins in the heart. J Mol Cell Cardiol 111:61-68 (2017). WB . PubMed: 28826663