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Signal Transduction Adapters Transmembrane

Anti-alpha Adaptin抗体[AP6] (ab2730)

  • Datasheet
  • SDS
Reviews (10)Q&A (4)References (21)

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Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AP6] (ab2730)
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AP6] (ab2730)
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AP6] (ab2730)
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AP6] (ab2730)
  • Flow Cytometry - Anti-alpha Adaptin antibody [AP6] (ab2730)

Key features and details

  • Mouse monoclonal [AP6] to alpha Adaptin
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Mouse, Human
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-alpha Adaptin抗体[AP6]
    参阅全部 alpha Adaptin 一抗
  • 描述

    小鼠单克隆抗体[AP6] to alpha Adaptin
  • 宿主

    Mouse
  • 特异性

    Detects assembly polypeptide 2 (AP2) It recognizes the products of both alpha-adaptin genes, alpha A and alpha C as well as an alternatively spliced isoform of alpha A found in neurons.
  • 经测试应用

    适用于: ICC/IF, Flow Cytmore details
  • 种属反应性

    与反应: Mouse, Human
    预测可用于: Non human primates
  • 免疫原

    Other Immunogen Type corresponding to alpha Adaptin. Purified adaptors.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度

    Affinity purified
  • 克隆

    单克隆
  • 克隆编号

    AP6
  • 同种型

    IgG1
  • 研究领域

    • Signal Transduction
    • Adapters
    • Transmembrane

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2730于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF (4)
Use a concentration of 5 µg/ml.
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

说明
ICC/IF
Use a concentration of 5 µg/ml.
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

靶标

  • 相关性

    Clathrin mediated endocytosis is the pathway by which many receptors for nutrients and hormones are internalized to be recycled or down regulated. During formation of clathrin coated membranes, clathrin co assembles with heterotetrameric molecules known as assembly polypeptides (APs) or adaptors which form a layer of protein coat between the clathrin lattice and the membrane. There are two characterized adaptors AP1 and AP2. AP1 is associated with clathrin coated vesicles at the trans Golgi network and AP2 is associated with the endocytic clathrin coated vesicles at the plasma membrane and has been shown to specifically interact with Shc and EGF receptor. AP2 is composed of four subunits, two separate 100 kDa gene products with similar domain structures (alpha and beta adaptin) and a 50 and 17 kDa subunit. There are two alpha adaptin genes, alpha A and alpha C which have a tissue specific pattern of expression.
  • 细胞定位

    Cytoplasmic
  • 数据库链接

    • Entrez Gene: 160 Human
    • Entrez Gene: 161 Human
    • Entrez Gene: 11771 Mouse
    • Entrez Gene: 11772 Mouse
    • Omim: 601026 Human
    • Omim: 607242 Human
    • SwissProt: O94973 Human
    • SwissProt: O95782 Human
    • SwissProt: P17426 Mouse
    • SwissProt: P17427 Mouse
    • Unigene: 19121 Human
    • Unigene: 6877 Mouse
    see all
  • 别名

    • 100 kDa coated vesicle protein A antibody
    • 100 kDa coated vesicle protein C antibody
    • Adapter related protein complex 2 subunit alpha 1 antibody
    • Adapter related protein complex 2 subunit alpha 2 antibody
    • Adaptin alpha A antibody
    • Adaptin alpha B antibody
    • Adaptin, alpha B antibody
    • Adaptor protein complex AP 2 alpha 1 subunit antibody
    • Adaptor protein complex AP 2 alpha 2 subunit antibody
    • Adaptor protein complex AP 2 subunit alpha 1 antibody
    • Adaptor related protein complex 2 alpha 1 subunit antibody
    • Adaptor related protein complex 2 alpha 2 subunit antibody
    • ADTAA antibody
    • ADTAB antibody
    • Alpha adaptin A antibody
    • Alpha adaptin C antibody
    • Alpha adaptin C; Huntingtin interacting protein J antibody
    • Alpha1 adaptin antibody
    • Alpha2 adaptin antibody
    • AP 2 complex subunit alpha 1 antibody
    • AP 2 complex subunit alpha 2 antibody
    • AP2 alpha antibody
    • AP2A1 antibody
    • AP2A2 antibody
    • CLAPA1 antibody
    • CLAPA2 antibody
    • Clathrin assembly protein complex 2 alpha A large chain antibody
    • Clathrin assembly protein complex 2 alpha C large chain antibody
    • Clathrin associated/assembly/adaptor protein large alpha 1 antibody
    • Clathrin associated/assembly/adaptor protein large alpha 2 antibody
    • HIP 9 antibody
    • HIP9 antibody
    • Huntingtin interacting protein antibody
    • Huntingtin interacting protein HYPJ antibody
    • Huntingtin interacting protein J antibody
    • Huntingtin yeast partner J antibody
    • HYPJ antibody
    • Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit antibody
    • Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit antibody
    see all

图片

  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AP6] (ab2730)
    Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AP6] (ab2730)
    Immunofluorescent analysis of alpha Adaptin using alpha Adaptin Monoclonal Antibody (AP6) (ab2730) shows staining in U251 Cells. alpha Adaptin (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing alpha Adaptin (ab2730) at a dilution of 1:20 over night at 4C and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AP6] (ab2730)
    Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AP6] (ab2730)
    Immunofluorescent analysis of alpha Adaptin using alpha Adaptin Monoclonal Antibody (AP6) (ab2720) shows staining in MCF-7 Cells. alpha Adaptin (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing alpha Adaptin (ab2730) at a dilution of 1:20 over night at 4 C and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AP6] (ab2730)
    Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AP6] (ab2730)
    Immunofluorescent analysis of alpha Adaptin using alpha Adaptin Monoclonal Antibody (AP6) (ab2730) shows staining in Hela Cells. alpha Adaptin (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing alpha Adaptin (ab2720) at a dilution of 1:20 over night at 4 C and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AP6] (ab2730)
    Immunocytochemistry/ Immunofluorescence - Anti-alpha Adaptin antibody [AP6] (ab2730)
    ICC/IF image of ab2730 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2730, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Flow Cytometry - Anti-alpha Adaptin antibody [AP6] (ab2730)
    Flow Cytometry - Anti-alpha Adaptin antibody [AP6] (ab2730)

    Overlay histogram showing MCF7 cells stained with ab2730 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2730, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

实验方案

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (21)

发表研究结果有使用 ab2730?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab2730 被引用在 21 文献中.

  • Wagner W  et al. Myosin VI Drives Clathrin-Mediated AMPA Receptor Endocytosis to Facilitate Cerebellar Long-Term Depression. Cell Rep 28:11-20.e9 (2019). PubMed: 31269433
  • Charnley M  et al. A new role for Notch in the control of polarity and asymmetric cell division of developing T cells. J Cell Sci 133:N/A (2019). PubMed: 31578237
  • Evergren E  et al. Eps15R and clathrin regulate EphB2-mediated cell repulsion. Traffic 19:44-57 (2018). PubMed: 28972287
  • Hugo SE & Schlegel A A Genetic Model to Study Increased Hexosamine Biosynthetic Flux. Endocrinology 158:2420-2426 (2017). PubMed: 28582574
  • Humphries AC  et al. Cdc42 and the Rho GEF intersectin-1 collaborate with Nck to promote N-WASP-dependent actin polymerisation. J Cell Sci 127:673-85 (2014). PubMed: 24284073
View all Publications for this product

客户评价及客户问答

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1-4 of 4 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha Adaptin antibody [AP6]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (undifferentiated Human ES cells on MEFs)
Permeabilization
Yes - in blocking buffer
Specification
undifferentiated Human ES cells on MEFs
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

提交于 Feb 01 2016

Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha Adaptin antibody [AP6]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEPG2)
Specification
HEPG2
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

提交于 Nov 18 2015

Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha Adaptin [AP6] antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
0.1% BSA and 1% serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 16°C
Sample
Mouse Cell (mouse embryonic stem cell)
Specification
mouse embryonic stem cell
Permeabilization
Yes - 0.1% triton x
Fixative
methanol/acetone
Read More

Abcam user community

Verified customer

提交于 May 19 2014

Immunocytochemistry/ Immunofluorescence

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 22°C
Sample
Human Cell (HeLa)
Specification
HeLa
Permeabilization
Yes - 0.2% Triton in PBS
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

提交于 Mar 05 2013

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