Anti-alpha A Crystallin/CRYAA抗体(ab5595)
Key features and details
- Rabbit polyclonal to alpha A Crystallin/CRYAA
- Reacts with: Rat, Cow
- Isotype: IgG
概述
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产品名称
Anti-alpha A Crystallin/CRYAA抗体
参阅全部 alpha A Crystallin/CRYAA 一抗 -
描述
兔多克隆抗体to alpha A Crystallin/CRYAA -
宿主
Rabbit -
特异性
This antibody is not expected to detect the non-phosphorylated form of the protein. Please contact our Scientific Support team for more information. -
种属反应性
与反应: Rat, Cow
预测可用于: Sheep, Rabbit, Horse, Guinea pig, Hamster, Cat, Dog, Human, Pig, Rhesus monkey, Elephant -
免疫原
Full length native protein (purified) corresponding to Cow alpha A Crystallin/CRYAA (phospho ). Purified alpha-A Crystallin from bovine lens.
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常规说明
This product was previously labelled as alpha A Crystallin
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: PBS, 3% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
Primary antibody说明
Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens. The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha-crystallin is the largest of the crystallins and is composed of 2 primary gene products--alpha-A and alpha-B. There are at least 5 different proteins comprising the beta-crystallins. The gamma-crystallins are monomeric, but there are at least 5 gamma crystallins identified in bovine and rat lens. Alpha-Crystallin comprises 40% of total lens protein composition. In addition to maintaining proper refractive index, it also functions in a chaperone like manner by preventing the formation of aggregates possibly leading to cataract formation. It is believed that the phosphorylated states of the alpha-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. Alpha-B crystallin has been linked to Alexander’s disease where it accumulates in brain cells of those afflicted. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
靶标
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功能
May contribute to the transparency and refractive index of the lens. -
疾病相关
Defects in CRYAA are a cause of cataract autosomal dominant (ADC) [MIM:604219]. Cataract is an opacification of the crystalline lens of the eye that frequently results in visual impairment or blindness. Opacities vary in morphology, are often confined to a portion of the lens, and may be static or progressive. In general, the more posteriorly located and dense an opacity, the greater the impact on visual function. Cataract is the most common treatable cause of visual disability in childhood. -
序列相似性
Belongs to the small heat shock protein (HSP20) family. -
翻译后修饰
O-glycosylated; contains N-acetylglucosamine side chains.
Deamidation of Asn-101 in lens occurs mostly during the first 30 years of age, followed by a small additional amount of deamidation (approximately 5%) during the next approximately 38 years, resulting in a maximum of approximately 50% deamidation during the lifetime of the individual.
Phosphorylation on Ser-122 seems to be developmentally regulated. Absent in the first months of life, it appears during the first 12 years of human lifetime. The relative amount of phosphorylated form versus unphosphorylated form does not change over the lifetime of the individual. -
细胞定位
Cytoplasm. Nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles. - Information by UniProt
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数据库链接
- Entrez Gene: 281718 Cow
- Entrez Gene: 100135506 Guinea pig
- Entrez Gene: 1409 Human
- Entrez Gene: 100009294 Rabbit
- Entrez Gene: 24273 Rat
- Omim: 123580 Human
- SwissProt: P02470 Cow
- SwissProt: P02498 Elephant
see all -
别名
- Acry 1 antibody
- Alpha crystallin A chain antibody
- Alpha-crystallin A chain antibody
see all
数据表及文件
文献 (11)
ab5595 被引用在 11 文献中.
- Mao Z et al. MicroRNA-155 inhibition up-regulates LEPR to inhibit osteoclast activation and bone resorption via activation of AMPK in alendronate-treated osteoporotic mice. IUBMB Life N/A:N/A (2019). PubMed: 31317664
- Schlotterer A et al. Methylglyoxal induces retinopathy-type lesions in the absence of hyperglycemia: studies in a rat model. FASEB J N/A:fj201801146RR (2018). PubMed: 30485119
- Zayas-Santiago A et al. Localization of aA-Crystallin in Rat Retinal Müller Glial Cells and Photoreceptors. Microsc Microanal 24:545-552 (2018). PubMed: 30253817
- Yuan L et al. CRISPR/Cas9-Mediated Mutation of aA-Crystallin Gene Induces Congenital Cataracts in Rabbits. Invest Ophthalmol Vis Sci 58:BIO34-BIO41 (2017). PubMed: 28475701
- Nakazawa Y et al. Effect of hesperetin on chaperone activity in selenite-induced cataract. Open Med (Wars) 11:183-189 (2016). PubMed: 28352791