Anti-alpha A Crystallin/CRYAA抗体(ab5595)

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Key features and details

  • Rabbit polyclonal to alpha A Crystallin/CRYAA
  • Suitable for: IHC-P
  • Reacts with: Rat, Cow
  • Isotype: IgG

概述

  • 产品名称

    Anti-alpha A Crystallin/CRYAA抗体
    参阅全部 alpha A Crystallin/CRYAA 一抗

  • 描述

    兔多克隆抗体to alpha A Crystallin/CRYAA

  • 宿主

    Rabbit

  • 特异性

    This antibody is not expected to detect the non-phosphorylated form of the protein. Please contact our Scientific Support team for more information.

  • 经测试应用

    适用于: IHC-Pmore details

  • 种属反应性

    与反应: Rat, Cow
    预测可用于: Sheep, Rabbit, Horse, Guinea pig, Hamster, Cat, Dog, Human, Pig, Rhesus monkey, Elephant

  • 免疫原

    Full length native protein (purified) corresponding to Cow alpha A Crystallin/CRYAA (phospho ). Purified alpha-A Crystallin from bovine lens.

  • 常规说明

     This product was previously labelled as alpha A Crystallin

     

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: PBS, 3% BSA
  • Concentration information loading...

  • 纯度

    Immunogen affinity purified

  • Primary antibody说明

    Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens. The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha-crystallin is the largest of the crystallins and is composed of 2 primary gene products--alpha-A and alpha-B. There are at least 5 different proteins comprising the beta-crystallins. The gamma-crystallins are monomeric, but there are at least 5 gamma crystallins identified in bovine and rat lens. Alpha-Crystallin comprises 40% of total lens protein composition. In addition to maintaining proper refractive index, it also functions in a chaperone like manner by preventing the formation of aggregates possibly leading to cataract formation. It is believed that the phosphorylated states of the alpha-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. Alpha-B crystallin has been linked to Alexander’s disease where it accumulates in brain cells of those afflicted.

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

Our Abpromise guarantee covers the use of ab5595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 10 µg/ml.

靶标

  • 功能

    May contribute to the transparency and refractive index of the lens.

  • 疾病相关

    Defects in CRYAA are a cause of cataract autosomal dominant (ADC) [MIM:604219]. Cataract is an opacification of the crystalline lens of the eye that frequently results in visual impairment or blindness. Opacities vary in morphology, are often confined to a portion of the lens, and may be static or progressive. In general, the more posteriorly located and dense an opacity, the greater the impact on visual function. Cataract is the most common treatable cause of visual disability in childhood.

  • 序列相似性

    Belongs to the small heat shock protein (HSP20) family.

  • 翻译后修饰

    O-glycosylated; contains N-acetylglucosamine side chains.
    Deamidation of Asn-101 in lens occurs mostly during the first 30 years of age, followed by a small additional amount of deamidation (approximately 5%) during the next approximately 38 years, resulting in a maximum of approximately 50% deamidation during the lifetime of the individual.
    Phosphorylation on Ser-122 seems to be developmentally regulated. Absent in the first months of life, it appears during the first 12 years of human lifetime. The relative amount of phosphorylated form versus unphosphorylated form does not change over the lifetime of the individual.

  • 细胞定位

    Cytoplasm. Nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • Acry 1 antibody
    • Alpha crystallin A chain antibody
    • Alpha-crystallin A chain antibody
    • CRYA 1 antibody
    • CRYA1 antibody
    • CRYAA antibody
    • CRYAA_HUMAN antibody
    • Crystallin alpha 1 antibody
    • Crystallin alpha A antibody
    • Heat shock protein beta 4 antibody
    • Heat shock protein beta-4 antibody
    • HSPB 4 antibody
    • HspB4 antibody
    • short form antibody
    • Zonular Central Nuclear Cataract antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha A Crystallin/CRYAA antibody (ab5595)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha A Crystallin/CRYAA antibody (ab5595)Image from Dr D Burgess et al, BMC Dev Biol. 2010 Jan 27;10:13, Fig 5.

    ab5595 staining alpha A Crystallin/CRYAA in non-transgenic 13.5 mouse embryos by Immunohistochemistry (PFA fixed, paraffin embedded sections).Heads of mice were removed, fixed in 10% formalin, dehydrated, embedded in paraffin, sectioned (5-7 µm).Slides containing ocular sections from non-transgenic embryos were first deparaffinized and rehydrated. Antigens were retrieved by microwave treatment in 10 mM Sodium Citrate buffer (pH 6.0). Following antigen retrieval, the tissue sections were blocked with 10% normal horse serum for 30 minutes, at room temperature. The slides were then incubated with ab5595 at a 1/10,000 dilution overnight at 4°C. Following brief washes in PBS, the slides were incubated with the appropriate biotinylated-secondary antibodies for 30 minutes at 37°C. Antigen-antibody complexes were then detected using streptavidin-linked Alexa 594 (red) at 1/1000 dilution. Sections were mounted using anti-fade media containing DAPI (blue). Images were captured usin

文献 (11)

发表研究结果有使用 ab5595？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab5595 被引用在 11 文献中.

  • Mao Z  et al. MicroRNA-155 inhibition up-regulates LEPR to inhibit osteoclast activation and bone resorption via activation of AMPK in alendronate-treated osteoporotic mice. IUBMB Life N/A:N/A (2019). PubMed: 31317664
  • Schlotterer A  et al. Methylglyoxal induces retinopathy-type lesions in the absence of hyperglycemia: studies in a rat model. FASEB J N/A:fj201801146RR (2018). PubMed: 30485119
  • Zayas-Santiago A  et al. Localization of aA-Crystallin in Rat Retinal Müller Glial Cells and Photoreceptors. Microsc Microanal 24:545-552 (2018). PubMed: 30253817
  • Yuan L  et al. CRISPR/Cas9-Mediated Mutation of aA-Crystallin Gene Induces Congenital Cataracts in Rabbits. Invest Ophthalmol Vis Sci 58:BIO34-BIO41 (2017). PubMed: 28475701
  • Nakazawa Y  et al. Effect of hesperetin on chaperone activity in selenite-induced cataract. Open Med (Wars) 11:183-189 (2016). PubMed: 28352791
View all Publications for this product

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Question

Can this ab5595 detect the phospho and non-phospho proteins?

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Answer

Thanks for your email. I have checked the product file and it clearly states that this antibody does not detect the non-phosphorylated version of this protein, but there is no data in the file to indicate how this was determined. I looked up several early references that describe the production of this antibody and I'm not sure why it only recognizes the phosphorylated form of the alpha A crystalline. The paper that describes the original production of the antibody (Kato et al. Biochimica et Biophysica Acta 1991 v1080 p173-180) describes the alpha-A crystallin and how it is split into alpha-A1 and alpha-A2 forms, the A1 form being phosphorylated and A2 being non-phosphorylated. Apparently the A1 form was used as the immunogen for the production of PA1-009. The A1 form is phosphorylated at serine residues exclusively but as this is a polyclonal antibody I would expect other epitopes to exist that are not based on phosphorylation. I can no longer contact the person that generated this antibody, but I looked through our records and saw the statement that the antibody recognizes only the phosphorylated form several times, so I can only assume this has been well characterized. Sorry I don't have a more definitive answer for you but I hope this helps.

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Question

I received this antibody as a powder, instead of a liquid as before.

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Answer

Thank you for contacting us. I tried to return your call, but there was no answer. This antibody is being sold in lyophilized form. You may resuspend it in PBS to the desired concentration. The tube contains 100ug of affinity-purified antibody lyophilized from PBS with BSA and azide added. When resuspending, please do not vortex, as this could damage the antibody. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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