Key features and details
- Rabbit polyclonal to alpha A Crystallin/CRYAA
- Reacts with: Rat, Cow
- Isotype: IgG
产品名称Anti-alpha A Crystallin/CRYAA抗体
参阅全部 alpha A Crystallin/CRYAA 一抗
描述兔多克隆抗体to alpha A Crystallin/CRYAA
特异性This antibody is not expected to detect the non-phosphorylated form of the protein. Please contact our Scientific Support team for more information.
种属反应性与反应: Rat, Cow
预测可用于: Sheep, Rabbit, Horse, Guinea pig, Hamster, Cat, Dog, Human, Pig, Rhesus monkey, Elephant
Full length native protein (purified) corresponding to Cow alpha A Crystallin/CRYAA (phospho ). Purified alpha-A Crystallin from bovine lens.
This product was previously labelled as alpha A Crystallin
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.05% Sodium azide
Constituents: PBS, 3% BSA
Concentration information loading...
纯度Immunogen affinity purified
Primary antibody说明Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens. The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha-crystallin is the largest of the crystallins and is composed of 2 primary gene products--alpha-A and alpha-B. There are at least 5 different proteins comprising the beta-crystallins. The gamma-crystallins are monomeric, but there are at least 5 gamma crystallins identified in bovine and rat lens. Alpha-Crystallin comprises 40% of total lens protein composition. In addition to maintaining proper refractive index, it also functions in a chaperone like manner by preventing the formation of aggregates possibly leading to cataract formation. It is believed that the phosphorylated states of the alpha-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. Alpha-B crystallin has been linked to Alexander’s disease where it accumulates in brain cells of those afflicted.
功能May contribute to the transparency and refractive index of the lens.
疾病相关Defects in CRYAA are a cause of cataract autosomal dominant (ADC) [MIM:604219]. Cataract is an opacification of the crystalline lens of the eye that frequently results in visual impairment or blindness. Opacities vary in morphology, are often confined to a portion of the lens, and may be static or progressive. In general, the more posteriorly located and dense an opacity, the greater the impact on visual function. Cataract is the most common treatable cause of visual disability in childhood.
序列相似性Belongs to the small heat shock protein (HSP20) family.
翻译后修饰O-glycosylated; contains N-acetylglucosamine side chains.
Deamidation of Asn-101 in lens occurs mostly during the first 30 years of age, followed by a small additional amount of deamidation (approximately 5%) during the next approximately 38 years, resulting in a maximum of approximately 50% deamidation during the lifetime of the individual.
Phosphorylation on Ser-122 seems to be developmentally regulated. Absent in the first months of life, it appears during the first 12 years of human lifetime. The relative amount of phosphorylated form versus unphosphorylated form does not change over the lifetime of the individual.
细胞定位Cytoplasm. Nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
- Information by UniProt
- Acry 1 antibody
- Alpha crystallin A chain antibody
- Alpha-crystallin A chain antibody
ab5595 被引用在 11 文献中.
- Mao Z et al. MicroRNA-155 inhibition up-regulates LEPR to inhibit osteoclast activation and bone resorption via activation of AMPK in alendronate-treated osteoporotic mice. IUBMB Life N/A:N/A (2019). PubMed: 31317664
- Schlotterer A et al. Methylglyoxal induces retinopathy-type lesions in the absence of hyperglycemia: studies in a rat model. FASEB J N/A:fj201801146RR (2018). PubMed: 30485119
- Zayas-Santiago A et al. Localization of aA-Crystallin in Rat Retinal Müller Glial Cells and Photoreceptors. Microsc Microanal 24:545-552 (2018). PubMed: 30253817
- Yuan L et al. CRISPR/Cas9-Mediated Mutation of aA-Crystallin Gene Induces Congenital Cataracts in Rabbits. Invest Ophthalmol Vis Sci 58:BIO34-BIO41 (2017). PubMed: 28475701
- Nakazawa Y et al. Effect of hesperetin on chaperone activity in selenite-induced cataract. Open Med (Wars) 11:183-189 (2016). PubMed: 28352791