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Tags & Cell Markers Subcellular Markers Plasma Membrane

Anti-alpha 1 Sodium Potassium ATPase抗体[464.6] (ab7671)

  • Datasheet
  • SDS
Reviews (41)Q&A (39)References (287)

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Immunocytochemistry - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)
  • Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)
  • Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)
  • Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)

Key features and details

  • Mouse monoclonal [464.6] to alpha 1 Sodium Potassium ATPase
  • Suitable for: IHC-P, WB, ICC
  • Reacts with: Mouse, Rat, Rabbit, Human, Pig
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

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概述

  • 产品名称

    Anti-alpha 1 Sodium Potassium ATPase抗体[464.6]
    参阅全部 alpha 1 Sodium Potassium ATPase 一抗
  • 描述

    小鼠单克隆抗体[464.6] to alpha 1 Sodium Potassium ATPase
  • 宿主

    Mouse
  • 经测试应用

    适用于: IHC-P, WB, ICCmore details
  • 种属反应性

    与反应: Mouse, Rat, Rabbit, Human, Pig
    预测可用于: Sheep, Dog, Xenopus laevis, Monkey
  • 免疫原

    Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • This antibody gave a positive signal in the following tissue lysates: human kidney, rabbit heart, human brain and human brain membrane.This antibody also gave a positive signal in Porcine proximal tubule lysate.
  • 常规说明

    This antibody clone [464.6] is manufactured by Abcam.

    If you require this antibody in a different buffer formulation or a different conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine

    Some batches contain 6.97% L-Arginine as a stabilizing agent. For lot-specific buffer information, please contact our Scientific Support team.
  • Concentration information loading...
  • 纯度

    Protein G purified
  • 克隆

    单克隆
  • 克隆编号

    464.6
  • 同种型

    IgG1
  • 轻链类型

    kappa
  • 研究领域

    • Tags & Cell Markers
    • Subcellular Markers
    • Plasma Membrane
    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • ATPases
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • Alternative Versions

    • Alexa Fluor® 647 Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab196695)
    • HRP Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab196696)
    • Alexa Fluor® 488 Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab197496)
    • Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - BSA and Azide free (ab237969)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7671于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P (4)
Use a concentration of 5 µg/ml.
WB (23)
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 112 kDa. Abcam recommends using 5% BSA as the blocking agent.
ICC
Use a concentration of 1 - 10 µg/ml.

We recommend Methanol fixation and Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) preadsorbed (ab150118) secondary antibody.

说明
IHC-P
Use a concentration of 5 µg/ml.
WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 112 kDa. Abcam recommends using 5% BSA as the blocking agent.
ICC
Use a concentration of 1 - 10 µg/ml.

We recommend Methanol fixation and Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) preadsorbed (ab150118) secondary antibody.

靶标

  • 功能

    This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
  • 序列相似性

    Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.
  • 翻译后修饰

    Phosphorylation on Tyr-10 modulates pumping activity.
  • 细胞定位

    Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession P05023 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 403992 Dog
    • Entrez Gene: 476 Human
    • Entrez Gene: 11928 Mouse
    • Entrez Gene: 397481 Pig
    • Entrez Gene: 24211 Rat
    • Entrez Gene: 443381 Sheep
    • Entrez Gene: 399285 Xenopus laevis
    • Omim: 182310 Human
    • SwissProt: P05023 Human
    • SwissProt: Q8VDN2 Mouse
    • SwissProt: P06685 Rat
    • SwissProt: Q92123 Xenopus laevis
    • Unigene: 371889 Human
    • Unigene: 280103 Mouse
    • Unigene: 217534 Rat
    • Unigene: 2992 Rat
    • Unigene: 3792 Xenopus laevis
    see all
  • 别名

    • A1A1 antibody
    • AT1A1 antibody
    • AT1A1_HUMAN antibody
    • ATP1A1 antibody
    • Atpa-1 antibody
    • ATPase Na+/K+ transporting alpha 1 polypeptide antibody
    • ATPase Na+/K+ transporting subunit alpha 1 antibody
    • BC010319 antibody
    • EC 3.6.3.9 antibody
    • MGC3285 antibody
    • MGC38419 antibody
    • MGC51750 antibody
    • Na K ATPase alpha A catalytic polypeptide antibody
    • Na K ATPase catalytic subunit alpha A protein antibody
    • Na(+)/K(+) ATPase 1 antibody
    • Na(+)/K(+) ATPase alpha-1 subunit antibody
    • Na+, K+ ATPase alpha subunit antibody
    • Na+/K+ ATPase alpha 1 subunit antibody
    • Na+/K+ ATPase 1 antibody
    • Na,K ATPase alpha 1 subunit antibody
    • Nkaa1b antibody
    • Sodium potassium ATPase alpha 1 polypeptide antibody
    • Sodium pump 1 antibody
    • Sodium pump subunit alpha-1 antibody
    • sodium-potassium ATPase catalytic subunit alpha-1 antibody
    • Sodium/potassium-transporting ATPase subunit alpha-1 antibody
    see all

图片

  • Immunocytochemistry - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)
    Immunocytochemistry - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)

    ab7671 staining alpha 1 Sodium Potassium ATPase in Hek293 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab7671 at 1µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Also suitable in cells fixed with 100% methanol (5 min).

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a single confocal section is shown.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)This image is courtesy of an Abreview submitted by Anne Sailer

    ab7671 staining alpha 1 Sodium Potassium ATPase in human formaldehyde tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% BSA for 12 hours at 4°C; antigen retrieval was by heat mediation in a buffer pH 9. Samples were incubated with primary antibody (5µg/ml in 5% BSA) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated donkey anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)
    Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)
    Lanes 1-3 : Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671) at 1 µg/ml
    Lanes 4-6 : Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671) at 2 µg/ml
    Lanes 7-9 : Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671) at 5 µg/ml

    Lanes 1 & 4 & 7 : Human brain tissue lysate - total protein (ab29466)
    Lanes 2 & 5 & 8 : Brain (Mouse) Tissue Lysate (ab27253)
    Lanes 3 & 6 & 9 : Brain (Rat) Tissue Lysate (ab7942)

    Lysates/proteins at 20 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 112 kDa


    Exposure time: 3 minutes
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)
    IHC image of Ab7671 staining in Human Kidney Carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with Ab7671, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)
    Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)
    All lanes : alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker at 10 µg/ml

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : Human kidney tissue lysate - total protein (ab30203)
    Lane 3 : Heart (Rabbit) Whole Cell Lysate - normal tissue (ab29072)
    Lane 4 : Human brain tissue lysate - total protein (ab29466)
    Lane 5 : Brain (Human) Membrane Lysate - adult normal tissue

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 112 kDa
    Observed band size: 100 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes
  • Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)
    Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)
    Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671) at 1/5000 dilution + Porcine proximal tubule lysate

    Predicted band size: 112 kDa



    Western blot analysis detecting Na, K-ATP-ase (alpha) in porcine proximal tubule protein, using Ab7671. Band is at 112 kDa.

实验方案

  • Recommended protocol for WB with ab7671
  • Mouse on Mouse staining protocol

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (287)

发表研究结果有使用 ab7671?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab7671 被引用在 287 文献中.

  • Ando S  et al. Rubicon regulates A2E-induced autophagy impairment in the retinal pigment epithelium implicated in the pathology of age-related macular degeneration. Biochem Biophys Res Commun 551:148-154 (2021). PubMed: 33740621
  • Wang LL  et al. Role of calcitonin gene-related peptide in pain regulation in the parabrachial nucleus of naive rats and rats with neuropathic pain. Toxicol Appl Pharmacol 414:115428 (2021). PubMed: 33524449
  • Lee J  et al. Muscarinic Receptors and BK Channels Are Affected by Lipid Raft Disruption of Salivary Gland Cells. Int J Mol Sci 22:N/A (2021). PubMed: 33946369
  • Shimomura M  et al. Decreased muscle-derived musclin by chronic resistance exercise is associated with improved insulin resistance in rats with type 2 diabetes. Physiol Rep 9:e14823 (2021). PubMed: 33955191
  • Zhang H  et al. A subcellular map of the human kinome. Elife 10:N/A (2021). PubMed: 33988507
View all Publications for this product

客户评价及客户问答

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1-10 of 80 Abreviews or Q&A

Western blot abreview for Anti-alpha 1 Sodium Potassium ATPase antibody [464.6]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - other (Plasma membrane sample from PC-3 cells)
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Loading amount
40 µg
Specification
Plasma membrane sample from PC-3 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Dr. Armen Petrosyan

Verified customer

提交于 Dec 07 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha 1 Sodium Potassium ATPase antibody [464.6]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Rat hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Read More

Dr. Armen Petrosyan

Verified customer

提交于 Nov 25 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha 1 Sodium Potassium ATPase antibody [464.6]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mice hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Mice hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Read More

Dr. Armen Petrosyan

Verified customer

提交于 Oct 07 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-alpha 1 Sodium Potassium ATPase antibody [464.6]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization
No
Specification
Liver
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Fixative
Formalin
Read More

Abcam user community

Verified customer

提交于 Mar 11 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-alpha 1 Sodium Potassium ATPase antibody [464.6]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Balb/c Pancreas section)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization
No
Specification
Balb/c Pancreas section
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2.5%
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

提交于 Jan 24 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha 1 Sodium Potassium ATPase antibody [464.6]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (B16 mice tumor cells)
Permeabilization
Yes - 0.25% Triton-X100 10 minutes
Specification
B16 mice tumor cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

提交于 Jan 19 2017

Western blot abreview for Anti-alpha 1 Sodium Potassium ATPase antibody [464.6]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (OV90 cells)
Gel Running Conditions
Reduced Denaturing (4-20% Tris-Glycin gel)
Loading amount
1e+006 cells
Specification
OV90 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

提交于 Dec 06 2016

Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (N87 cell line)
Permeabilization
Yes - 0.25% Triton X-100
Specification
N87 cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

提交于 Sep 14 2016

Western blot abreview for Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker

Good
Abreviews
Abreviews
Application
Western blot
Sample
Rat Cell lysate - whole cell (cortex)
Gel Running Conditions
Reduced Denaturing (7%)
Loading amount
10 µg
Specification
cortex
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 23°C
Read More

Abcam user community

Verified customer

提交于 Mar 21 2016

Question

Thank you very much for the reply.
I'm working with membrane samples, would they also be heated to 95-100C for 10 minutes?
How come the datasheet for this product warns "Do not boil the protein samples, as boiling causes aggregation of the Na,K-ATPase
(60 degrees Celcius appears to work fine). The aggregate band will appear at ˜150 kDa on Western Blots." if samples can be boiled?
Kind regards,

Read More

Abcam community

Verified customer

Asked on Jul 18 2012

Answer

Thank you for your response.

Our Lab regularly test this product on whole cell lysates and heat the samples 95-100C for 10 minutes. The signal is very clear and distinctive. My personal experience is that plasmamebrane protein can be heating for 5 min. As I have mentioned in my previous e-mail, this comment was published long time ago part of a customer's feedback.

If you need any further assistance, please do not hesitate to contact me.

Read More

Abcam Scientific Support

回复于 Jul 18 2012

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