Anti-alpha 1 Sodium Potassium ATPase抗体[464.6] (ab7671)
![Immunocytochemistry - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)](https://www.abcam.cn/ps/products/7/ab7671/Images/ab7671-18-anti-alpha-1-sodium-potassium-atpase-4646-immunocytochemistry-antibody-hek293-human.jpg "Immunocytochemistry - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)")
Key features and details
- Mouse monoclonal [464.6] to alpha 1 Sodium Potassium ATPase
- Suitable for: IHC-P, WB, ICC
- Reacts with: Mouse, Rat, Rabbit, Human, Pig
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
Anti-alpha 1 Sodium Potassium ATPase抗体[464.6]
参阅全部 alpha 1 Sodium Potassium ATPase 一抗 -
描述
小鼠单克隆抗体[464.6] to alpha 1 Sodium Potassium ATPase -
宿主
Mouse -
经测试应用
适用于: IHC-P, WB, ICCmore details -
种属反应性
与反应: Mouse, Rat, Rabbit, Human, Pig
预测可用于: Sheep, Dog, Xenopus laevis, Monkey
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免疫原
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- This antibody gave a positive signal in the following tissue lysates: human kidney, rabbit heart, human brain and human brain membrane.This antibody also gave a positive signal in Porcine proximal tubule lysate.
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常规说明
This antibody clone [464.6] is manufactured by Abcam.
If you require this antibody in a different buffer formulation or a different conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Some batches contain 6.97% L-Arginine as a stabilizing agent. For lot-specific buffer information, please contact our Scientific Support team. -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
464.6 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
- Alexa Fluor® 647 Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab196695)
- HRP Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab196696)
- Alexa Fluor® 488 Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab197496)
- Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - BSA and Azide free (ab237969)
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Compatible Secondaries
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Isotype control
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Related Products
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (4) |
Use a concentration of 5 µg/ml.
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| WB | (23) |
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 112 kDa. Abcam recommends using 5% BSA as the blocking agent.
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| ICC |
Use a concentration of 1 - 10 µg/ml.
We recommend Methanol fixation and Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) preadsorbed (ab150118) secondary antibody. |
| 说明 |
|---|
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IHC-P
Use a concentration of 5 µg/ml. |
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WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 112 kDa. Abcam recommends using 5% BSA as the blocking agent. |
|
ICC
Use a concentration of 1 - 10 µg/ml. We recommend Methanol fixation and Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) preadsorbed (ab150118) secondary antibody. |
靶标
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功能
This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. -
序列相似性
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily. -
翻译后修饰
Phosphorylation on Tyr-10 modulates pumping activity. -
细胞定位
Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 403992 Dog
- Entrez Gene: 476 Human
- Entrez Gene: 11928 Mouse
- Entrez Gene: 397481 Pig
- Entrez Gene: 24211 Rat
- Entrez Gene: 443381 Sheep
- Entrez Gene: 399285 Xenopus laevis
- Omim: 182310 Human
see all -
别名
- A1A1 antibody
- AT1A1 antibody
- AT1A1_HUMAN antibody
see all
图片
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Immunocytochemistry - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)
ab7671 staining alpha 1 Sodium Potassium ATPase in Hek293 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab7671 at 1µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 100% methanol (5 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a single confocal section is shown.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)](https://www.abcam.cn/ps/products/7/ab7671/Images/ab7671-240726-anti-alpha-1-sodium-potassium-atpase-antibody-4646-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)This image is courtesy of an Abreview submitted by Anne Sailerab7671 staining alpha 1 Sodium Potassium ATPase in human formaldehyde tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% BSA for 12 hours at 4°C; antigen retrieval was by heat mediation in a buffer pH 9. Samples were incubated with primary antibody (5µg/ml in 5% BSA) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated donkey anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.
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![Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)](https://www.abcam.cn/ps/products/7/ab7671/Images/alpha-1-Sodium-Potassium-ATPase-Primary-antibodies-ab7671-65.jpg)
Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)Lanes 1-3 : Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671) at 1 µg/ml
Lanes 4-6 : Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671) at 2 µg/ml
Lanes 7-9 : Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671) at 5 µg/ml
Lanes 1 & 4 & 7 : Human brain tissue lysate - total protein (ab29466)
Lanes 2 & 5 & 8 : Brain (Mouse) Tissue Lysate (ab27253)
Lanes 3 & 6 & 9 : Brain (Rat) Tissue Lysate (ab7942)
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 112 kDa
Exposure time: 3 minutes -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)](https://www.abcam.cn/ps/products/7/ab7671/Images/ab7671-13008-anti-alpha-1-sodium-potassium-atpase-antibody-4646-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)IHC image of Ab7671 staining in Human Kidney Carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with Ab7671, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
![Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)](https://www.abcam.cn/ps/products/7/ab7671/Images/alpha-1-Sodium-Potassium-ATPase-Primary-antibodies-ab7671-9.jpg)
Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)All lanes : alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker at 10 µg/ml
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : Human kidney tissue lysate - total protein (ab30203)
Lane 3 : Heart (Rabbit) Whole Cell Lysate - normal tissue (ab29072)
Lane 4 : Human brain tissue lysate - total protein (ab29466)
Lane 5 : Brain (Human) Membrane Lysate - adult normal tissue
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 112 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes -
![Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)](https://www.abcam.cn/ps/products/7/ab7671/Images/ab7671_3.jpg)
Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671) at 1/5000 dilution + Porcine proximal tubule lysate
Predicted band size: 112 kDa
Western blot analysis detecting Na, K-ATP-ase (alpha) in porcine proximal tubule protein, using Ab7671. Band is at 112 kDa.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (280)
ab7671 被引用在 280 文献中.
- Ando S et al. Rubicon regulates A2E-induced autophagy impairment in the retinal pigment epithelium implicated in the pathology of age-related macular degeneration. Biochem Biophys Res Commun 551:148-154 (2021). PubMed: 33740621
- Wang LL et al. Role of calcitonin gene-related peptide in pain regulation in the parabrachial nucleus of naive rats and rats with neuropathic pain. Toxicol Appl Pharmacol 414:115428 (2021). PubMed: 33524449
- Lee J et al. Muscarinic Receptors and BK Channels Are Affected by Lipid Raft Disruption of Salivary Gland Cells. Int J Mol Sci 22:N/A (2021). PubMed: 33946369
- Shimomura M et al. Decreased muscle-derived musclin by chronic resistance exercise is associated with improved insulin resistance in rats with type 2 diabetes. Physiol Rep 9:e14823 (2021). PubMed: 33955191
- Zhang H et al. A subcellular map of the human kinome. Elife 10:N/A (2021). PubMed: 33988507